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    Dimers of unsymmetrical cyanine dyes
    3.
    发明授权
    Dimers of unsymmetrical cyanine dyes 失效
    不对称花青染料的二聚体

    公开(公告)号：US5582977A

    公开(公告)日：1996-12-10

    申请号：US180763

    申请日：1994-01-06

    申请人： Stephen T. Yue ,  Iain D. Johnson ,  Richard P. Haugland

    发明人： Stephen T. Yue ,  Iain D. Johnson ,  Richard P. Haugland

    IPC分类号： C09B23/02 ,  G01N33/52 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C09B23/02 ,  G01N33/52

    摘要： The invention relates to dimers of unsymmetrical cyanine dyes, typically dimers of benzthiazole or benzoxazole derivatives, that exhibit enhanced fluorescence on binding with DNA or RNA, The dimers generally have the formula: ##STR1## where R.sup.1 and R.sup.2, which may be the same or different, are alkyl groups having 1-6 carbons;X is O, S, or N--R.sup.3, where R.sup.3 is H or an alkyl group having 1-6 carbons;Z is O, S, or N--R.sup.4, where R.sup.4 is H or an alkyl group having 1-6 carbons;n and s, which may be the same or different, =0, 1, or 2;Y is HC.dbd.CH; andp, m, q, and r=0 or 1, such that p+m=1 and q+r=1; andwhere -BRIDGE- has the general formula:--(CH.sub.2).sub..alpha. --[A.sup.1 --(CH.sub.2).sub..beta. --].sub.I [A.sup.2 --(CH.sub.2).sub..gamma. --].sub.II A.sup.3 --(CH.sub.2).sub..delta. --where .alpha., .beta., .gamma., and .delta., which may be the same or different, are integers greater than 1 and less than 5;I and II, which may be the same or different, =0 or 1; andA.sup.1, A.sup.2, and A.sup.3, which may be the same or different, are independently O; S; (CH.sub.2).sub..mu. where .mu.=0 or 1; --(NR.sup.5)-- where R.sup.5 is H or an alkyl group having 1-6 carbons; or--(N.sup.+ R.sup.6 R.sup.7)-- where R.sup.6 and R.sup.7, which may be the same or different, are independently hydrogen or an alkyl group having 1-6 carbons.

    摘要翻译： 本发明涉及不对称花青染料的二聚体，通常是苯并噻唑或苯并恶唑衍生物的二聚体，其在与DNA或RNA结合时表现出增强的荧光。二聚体通常具有下式：具有下式的烷基：具有 1-6个碳; X是O，S或N-R 3，其中R 3是H或具有1-6个碳的烷基; Z是O，S或N-R 4，其中R 4是H或具有1-6个碳的烷基; n和s可以相同或不同，= 0,1或2; Y为HC = CH; 和p，m，q和r = 0或1，使得p + m = 1和q + r = 1; 并且其中-BRIDGE具有以下通式： - （CH 2）a - [A 1-（CH 2）β - ] I [A2-（CH 2）γ - ] IIA 3 - （CH 2）δ-其中α，β，γ和 δ可以相同或不同，是大于1且小于5的整数; I和II可以相同或不同，= 0或1; 和可以相同或不同的A1，A2和A3独立地为O; S; （CH 2）m，其中mu = 0或1; - （NR5） - 其中R5是H或具有1-6个碳原子的烷基; 或 - （N + R6R7） - 其中R6和R7可以相同或不同，独立地为氢或具有1-6个碳原子的烷基。

    Unsymmetrical cyanine dyes with a cationic side chain
    4.
    发明授权
    Unsymmetrical cyanine dyes with a cationic side chain 失效
    具有阳离子侧链的不对称花青染料

    公开(公告)号：US5321130A

    公开(公告)日：1994-06-14

    申请号：US833006

    申请日：1992-02-10

    申请人： Stephen T. Yue ,  Iain D. Johnson ,  Zhijian Huang ,  Richard P. Haugland

    发明人： Stephen T. Yue ,  Iain D. Johnson ,  Zhijian Huang ,  Richard P. Haugland

    IPC分类号： C09B23/02 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C09B23/02 ,  Y10S435/968 ,  Y10S436/80

    摘要： The invention relates to unsymmetrical cyanine dyes with a cationic side chain, typically benzothiazole or benzoxazole derivatives, that exhibit enhanced fluorescence on binding with DNA or RNA, where such fluorescence can be used for evaluating the presence of nucleic acid polymers. The dyes generally have the formula: ##STR1## where R.sup.1 is an alkyl group having 1-6 carbons; X is 0, S, or NR.sup.2, where R.sup.2 is an alkyl group having 1-6 carbons, or CR.sup.3 R.sup.4, where R.sup.3 and R.sup.4, which may be the same or different, are alkyl groups having 1-6 carbons;n=0, 1, or 2;Z.sup.1 and Z.sup.2, which may be the same or different, are independently hydrogen, an alkyl group having 1-6 carbons, or aryl, or Z.sup.1 and Z.sup.2 taken in combination complete a 6-membered aromatic ring;Y is HC.dbd.CH; andeach of P and M=0 or 2, such that P+M=1; andTAIL is an aminoalkyl chain containing a backbone of 3-42 carbons and 1-5 positively charged nitrogens intermittently or equally spaced within the backbone, such that there are at least two carbons between sequential nitrogens.

    摘要翻译： 本发明涉及具有阳离子侧链（通常为苯并噻唑或苯并恶唑衍生物）的不对称花青染料，其在与DNA或RNA结合时表现出增强的荧光，其中该荧光可用于评估核酸聚合物的存在。 染料通常具有下式：其中R1是具有1-6个碳原子的烷基; X是0，S或NR2，其中R2是具有1-6个碳原子的烷基，或CR3R4，其中R3和R4可以相同或不同，是具有1-6个碳原子的烷基; n = 0,1或2; Z 1和Z 2可以相同或不同，独立地为氢，具有1-6个碳的烷基或芳基，或Z 1和Z 2组合形成6元芳环; Y为HC = CH; 并且P和M中的每一个= 0或2，使得P + M = 1; 并且TAIL是含有3-42个碳原子的主链和1-5个带正电荷的氮在主链内间歇或等间隔的氨基烷基链，使得在相继的氮之间存在至少两个碳。

    Competitive immunoassay
    7.
    发明授权
    Competitive immunoassay 有权
    竞争性免疫测定

    公开(公告)号：US07282339B2

    公开(公告)日：2007-10-16

    申请号：US10943463

    申请日：2004-09-17

    申请人： Joseph Beechem ,  Kyle Richard Gee ,  David Carl Hagen ,  Iain D Johnson ,  Hee-Chol Kang ,  Christina Pastula

    发明人： Joseph Beechem ,  Kyle Richard Gee ,  David Carl Hagen ,  Iain D Johnson ,  Hee-Chol Kang ,  Christina Pastula

    IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  C12Q1/42 ,  C07K1/13

    CPC分类号： G01N33/542 ,  C07F9/12 ,  C07F9/65318 ,  C07F9/65522 ,  C07F9/6596 ,  C07K16/46 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S530/807 ,  Y10T436/13

    摘要： The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.

    摘要翻译： 本发明提供配体检测试剂，配体类似物和用于测定样品中配体存在的方法。 配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物，其中配体类似物共价键合到报告分子。 该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质，其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。 取决于报道分子和配体结合抗体，报告分子被配体结合抗体或标记蛋白的标记部分淬灭，其中淬灭量与存在于 样品。 或者，配体类似物是荧光的，其中配体类似物在溶液中基本上是非荧光的，但当被配体结合抗体结合时，可检测信号增加。 在这种情况下，与目标配体的存在相比，测量了与消除淬灭相反的信号降低。