公开(公告)号：US20100009342A1

公开(公告)日：2010-01-14

申请号：US12501253

申请日：2009-07-10

Applicant: John Matthew MAURO ,  Thomas Harry STEINBERG ,  Lawrence I. GREENFIELD ,  Louis LEONG

Inventor： John Matthew MAURO ,  Thomas Harry STEINBERG ,  Lawrence I. GREENFIELD ,  Louis LEONG

IPC: C12Q1/70 ,  G01N33/68 ,  G01N33/00 ,  G01N33/92 ,  C12Q1/02 ,  G01N33/566 ,  G01N33/53

CPC classification number: C07K1/13 ,  G01N33/532 ,  Y10T436/143333

Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.

Abstract translation: 提供了通过向标记反应添加反应性标记竞争体来控制载体分子或固体支持物的标记度（DOL）的方法。 当将反应性标签竞争剂加入到标记溶液中时，竞争者与载体分子竞争，或与标签的固体支持物竞争，减少可用于缀合物与载体分子或固体支持物的标签数目。 这提供了可预测地改变载体分子或固体支持物的DOL的简便方法。

公开(公告)号：US20080176213A1

公开(公告)日：2008-07-24

申请号：US12057258

申请日：2008-03-27

Applicant: John Matthew Mauro ,  Thomas Harry Steinberg ,  Lawrence I. Greenfield ,  Louis Leong

Inventor： John Matthew Mauro ,  Thomas Harry Steinberg ,  Lawrence I. Greenfield ,  Louis Leong

IPC: C12Q1/00 ,  C12N5/00 ,  C12N5/02 ,  C12N1/38

CPC classification number: C07K1/13 ,  G01N33/532 ,  Y10T436/143333

Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.

Abstract translation: 提供了通过向标记反应添加反应性标记竞争体来控制载体分子或固体支持物的标记度（DOL）的方法。 当将反应性标签竞争剂加入到标记溶液中时，竞争者与载体分子竞争，或与标签的固体支持物竞争，减少可用于缀合物与载体分子或固体支持物的标签数目。 这提供了可预测地改变载体分子或固体支持物的DOL的简便方法。

公开(公告)号：US07432372B2

公开(公告)日：2008-10-07

申请号：US10980139

申请日：2004-11-01

Applicant: Robert Hardy Batchelor ,  Yue Ge ,  Iain D. Johnson ,  Wai-Yee Leung ,  Jixiang Liu ,  Brian Patch ,  Peter Aaron Emrys Smalley ,  Thomas Harry Steinberg

Inventor： Robert Hardy Batchelor ,  Yue Ge ,  Iain D. Johnson ,  Wai-Yee Leung ,  Jixiang Liu ,  Brian Patch ,  Peter Aaron Emrys Smalley ,  Thomas Harry Steinberg

IPC: C07D265/38

CPC classification number: C09B19/00 ,  C07D265/38 ,  C07D413/12 ,  C07D495/04 ,  C12Q1/28

Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.

Abstract translation: 本发明提供可用于各种测定形式的新型氟化聚砜化合物。 还提供了使用包括本发明化合物的化合物和试剂盒的方法以及详细说明化合物在一种或多种测定形式中的用途的说明书。