公开(公告)号：US07816148B2

公开(公告)日：2010-10-19

申请号：US11738437

申请日：2007-04-20

申请人： Andreas Manz ,  Luc J. Bousse

发明人： Andreas Manz ,  Luc J. Bousse

IPC分类号： G01N1/10 ,  B01L3/02

CPC分类号： B01L3/5027 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0403 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N35/08 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

摘要： Microfluidic devices and systems for affecting the serial to parallel conversion of materials introduced into the device or system. Material or materials to be converted from a serial orientation, e.g., a single channel, into a parallel orientation, e.g., multiple channels, are introduced into an open chamber or field in which containing flows of materials maintain the cohesiveness of the sample material plugs serially introduced into the open chamber. The sample material or materials are then redirected in the chamber toward and into a plurality of parallel channels that also communicate with the chamber.

摘要翻译： 用于影响引入设备或系统的材料的串行到并行转换的微流控器件和系统。 要从串行方向（例如单个通道）转换为平行取向（例如多个通道）的材料或材料被引入到开放的室或场中，其中包含材料流保持样品材料塞的连续性串联 引入开放室。 样品材料然后在室中被重新定向并且进入也与室连通的多个平行通道。

公开(公告)号：US07329111B2

公开(公告)日：2008-02-12

申请号：US10537238

申请日：2003-11-13

申请人： Emmanuel Delamarche ,  David Juncker ,  Bruno Michel ,  Heinz Schmid

发明人： Emmanuel Delamarche ,  David Juncker ,  Bruno Michel ,  Heinz Schmid

IPC分类号： B21C3/00

CPC分类号： B01L3/0262 ,  B01J2219/00369 ,  B01J2219/0038 ,  B01J2219/0043 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2300/0809 ,  B01L2300/089 ,  B01L2300/10 ,  B01L2400/022 ,  B01L2400/0403 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B05C5/02 ,  C40B60/14 ,  Y10T137/9682 ,  Y10T436/2575

摘要： A device for flowing a liquid on a surface comprises: a flow path. A first port supplies the liquid to one end of the flow path and applies a first port pressure for retaining the liquid when the flow path is remote from the surface. A second port receives the liquid from the other end of the flow path and applies a second port pressure such that the difference between the first and second negative port pressures is oriented to promote flow of the liquid from the first port to the second port via the flow path in response to the flow path being located proximal to the surface and the liquid in the device contacting the surface. The first and second port pressures are such that the liquid is drawn towards at least the second port in response to withdrawal of the flow path from the surface. Such devices may employ microfluidic technology and find application in surface patterning.

摘要翻译： 用于在表面上流动液体的装置包括：流动路径。 第一端口将液体供应到流路的一端，并且当流动路径远离表面时施加用于保持液体的第一端口压力。 第二端口从流动路径的另一端接收液体并施加第二端口压力，使得第一和第二负压力压力之间的差定向为促进液体从第一端口流经第二端口经由 响应于流动路径位于表面附近的流动路径以及与表面接触的装置中的液体。 第一和第二端口压力使得响应于流动路径从表面的撤回而将液体吸向至少第二端口。 这样的装置可以采用微流控技术并在表面图案化中找到应用。

公开(公告)号：US07282370B2

公开(公告)日：2007-10-16

申请号：US11207443

申请日：2005-08-18

申请人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael C. Pallas ,  Sydney Brenner

发明人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael C. Pallas ,  Sydney Brenner

IPC分类号： G01N21/76 ,  G01N21/00 ,  G01N37/00

CPC分类号： G01N21/253 ,  B01J2219/00274 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2035/00158 ,  Y10S435/81 ,  Y10T436/13 ,  Y10T436/143333

摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

摘要翻译： 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。 通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

公开(公告)号：US20060093697A1

公开(公告)日：2006-05-04

申请号：US10537238

申请日：2003-11-13

申请人： Emmanuel Delamarche ,  David Juncker ,  Bruno Michel ,  Heinz Schmid

发明人： Emmanuel Delamarche ,  David Juncker ,  Bruno Michel ,  Heinz Schmid

IPC分类号： B29C67/00

CPC分类号： B01L3/0262 ,  B01J2219/00369 ,  B01J2219/0038 ,  B01J2219/0043 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2300/0809 ,  B01L2300/089 ,  B01L2300/10 ,  B01L2400/022 ,  B01L2400/0403 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B05C5/02 ,  C40B60/14 ,  Y10T137/9682 ,  Y10T436/2575

摘要： A device for flowing a liquid on a surface comprises: a flow path. A first port supplies the liquid to one end of the flow path and applies a first port pressure for retaining the liquid when the flow path is remote from the surface. A second port receives the liquid from the other end of the flow path and applies a second port pressure such that the difference between the first and second negative port pressures is oriented to promote flow of the liquid from the first port to the second port via the flow path in response to the flow path being located proximal to the surface and the liquid in the device contacting the surface. The first and second port pressures are such that the liquid is drawn towards at least the second port in response to withdrawal of the flow path from the surface. Such devices may employ microfluidic technology and find application in surface patterning.

摘要翻译： 用于在表面上流动液体的装置包括：流动路径。 第一端口将液体供应到流路的一端，并且当流动路径远离表面时施加用于保持液体的第一端口压力。 第二端口从流动路径的另一端接收液体并施加第二端口压力，使得第一和第二负压力压力之间的差定向为促进液体从第一端口流经第二端口经由 响应于流动路径位于表面附近的流动路径以及与表面接触的装置中的液体。 第一和第二端口压力使得响应于流动路径从表面的撤回而将液体吸向至少第二端口。 这样的装置可以采用微流控技术并在表面图案化中找到应用。

公开(公告)号：US20060051876A1

公开(公告)日：2006-03-09

申请号：US11207443

申请日：2005-08-18

申请人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael Pallas ,  Sydney Brenner

发明人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael Pallas ,  Sydney Brenner

IPC分类号： G01N33/50

CPC分类号： G01N21/253 ,  B01J2219/00274 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2035/00158 ,  Y10S435/81 ,  Y10T436/13 ,  Y10T436/143333

摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

公开(公告)号：US20050045479A1

公开(公告)日：2005-03-03

申请号：US10821267

申请日：2004-04-08

申请人： Bernhard Weigl ,  Clinton Williams ,  Jon Hayenga ,  Ronald Bardell ,  Thomas Schulte

发明人： Bernhard Weigl ,  Clinton Williams ,  Jon Hayenga ,  Ronald Bardell ,  Thomas Schulte

IPC分类号： B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01F13/08 ,  B01L3/00 ,  B81B1/00 ,  F15C3/04 ,  F15C3/06 ,  F15C5/00 ,  F16K99/00 ,  G01N27/06 ,  G01N35/00 ,  G01L1/20 ,  B01D57/02 ,  C02F1/469

CPC分类号： G01N27/06 ,  B01F5/0646 ,  B01F5/0647 ,  B01F11/0082 ,  B01F13/0059 ,  B01F13/0093 ,  B01F13/0809 ,  B01L3/5023 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0636 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2400/0403 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/0466 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0688 ,  F15C3/04 ,  F15C3/06 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0028 ,  F16K99/003 ,  F16K99/0061 ,  F16K2099/0078 ,  F16K2099/008 ,  G01N2035/00237 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： A microfluidic device which operates without the need for an external power source. The device includes a body structure, at least one microscale channel within the structure, a port for introducing fluid into the channel, and a power source internal to the structure for propelling the fluid through the channel. Various structures are described which embody the invention.

摘要翻译： 在不需要外部电源的情况下工作的微流体装置。 该装置包括主体结构，结构内的至少一个微型通道，用于将流体引入通道的端口以及用于推动流体通过通道的结构内部的电源。 描述了体现本发明的各种结构。

公开(公告)号：US06743399B1

公开(公告)日：2004-06-01

申请号：US09684094

申请日：2000-10-06

申请人： Bernhard H. Weigl ,  Clinton L. Williams ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Thomas E. Schulte

发明人： Bernhard H. Weigl ,  Clinton L. Williams ,  Jon W. Hayenga ,  Ronald L. Bardell ,  Thomas E. Schulte

IPC分类号： B02L300

CPC分类号： G01N27/06 ,  B01F5/0646 ,  B01F5/0647 ,  B01F11/0082 ,  B01F13/0059 ,  B01F13/0093 ,  B01F13/0809 ,  B01L3/5023 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0636 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2400/0403 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/0466 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0688 ,  F15C3/04 ,  F15C3/06 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0028 ,  F16K99/003 ,  F16K99/0061 ,  F16K2099/0078 ,  F16K2099/008 ,  G01N2035/00237 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： A microfluidic device which operates without the need for an external power source. The device includes a body structure, at least one microscale channel within the structure, a port for introducing fluid into the channel, and a power source internal to the structure for propelling the fluid through the channel. Various structures are described which embody the invention.

摘要翻译： 在不需要外部电源的情况下工作的微流体装置。 该装置包括主体结构，结构内的至少一个微型通道，用于将流体引入通道的端口以及用于推动流体通过通道的结构内部的电源。 描述了体现本发明的各种结构。

公开(公告)号：US20020061529A1

公开(公告)日：2002-05-23

申请号：US09908131

申请日：2001-07-17

申请人： Lynx Therapeutics, Inc.

发明人： John Bridgham ,  Kevin P. Corcoran ,  George S. Golda ,  Sydney Brenner ,  Michael C. Pallas

IPC分类号： C12Q001/68 ,  G06K009/00 ,  C12M001/34

CPC分类号： G01N21/253 ,  B01J2219/00274 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2035/00158 ,  Y10S435/81 ,  Y10T436/13 ,  Y10T436/143333

摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

摘要翻译： 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒来进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。 通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

公开(公告)号：US20010055546A1

公开(公告)日：2001-12-27

申请号：US09888727

申请日：2001-06-25

发明人： Bernhard H. Weigl ,  Thomas Schulte ,  Ronald L. Bardell

IPC分类号： G01N021/05 ,  B01L003/02

CPC分类号： B01F5/0646 ,  B01F5/0647 ,  B01F11/0082 ,  B01F13/0059 ,  B01F13/0093 ,  B01F13/0809 ,  B01L3/502738 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0403 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/0655 ,  B01L2400/0688 ,  F15C3/04 ,  F15C3/06 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0028 ,  G01N27/06

摘要： A method and apparatus are disclosed for controlling a flow rate of a fluid sample having an unknown or variable viscosity. The fluid sample is provided as a first fluid flow to a microfluidic channel. A second fluid is provided to the channel as a sheath around the first fluid. In one embodiment, the second fluid is injected between the first fluid flow and an internal surface of the channel. In another embodiment, the second fluid completely circumscribes the first fluid as a parallel or sheath flow. The second fluid has a known viscosity selected for achieving a flow rate. The first fluid flows at the same rate as the achieved flow rate of the second fluid.

摘要翻译： 公开了用于控制具有未知或可变粘度的流体样品的流速的方法和装置。 流体样品作为第一流体流被提供到微流体通道。 第二流体作为围绕第一流体的护套提供到通道。 在一个实施例中，第二流体被注入在第一流体流和通道的内表面之间。 在另一个实施例中，第二流体完全围绕第一流体作为平行或鞘流。 第二流体具有选择用于实现流速的已知粘度。 第一流体以与获得的第二流体的流量相同的速率流动。

公开(公告)号：US12128407B2

公开(公告)日：2024-10-29

申请号：US16093468

申请日：2017-04-17

申请人： Vortex Biosciences, Inc.

发明人： Elodie Sollier ,  Michael Lee Kochersperger ,  Robert F. Englert ,  James Che ,  Kuo-Wei Huang ,  Michael Boyce-Jacino ,  Anatasia Neddersen ,  Anna Passernig ,  Bruce Richardson ,  Insang Choi

IPC分类号： B01L3/00 ,  G01N1/10 ,  G01N15/10 ,  G01N15/14 ,  G01N35/10

CPC分类号： B01L3/502761 ,  B01L3/502738 ,  G01N1/10 ,  G01N15/1484 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0403 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/084 ,  G01N2015/1006 ,  G01N35/1097

摘要： Microfluidic chips and cartridges and systems that include such chips are disclosed. In some embodiments, the chips include a microfluidic channel disposed in a substrate with the channel comprising at least one expansion region. The channel is configured to generate a vortex within the at least one expansion region in response to fluid through the microfluidic channel to trap cells or particles. The substrate in which the channel is formed may be relatively rigid to resist deformation.