公开(公告)号：US20240182955A1

公开(公告)日：2024-06-06

申请号：US18546431

申请日：2022-02-17

申请人： ACT GENOMICS (IP) LIMITED

发明人： AN HAU ,  PEI-YI LIN ,  DATSEN GEORGE WEI ,  HUA-CHIEN CHEN ,  SHU-JEN CHEN ,  CHIH-YI YANG

IPC分类号： C12Q1/6827 ,  C12Q1/686

CPC分类号： C12Q1/6827 ,  C12Q1/686 ,  C12Q2600/156

摘要： The present disclosure relates to the fields of a method a kit for molecular diagnostics and genomics. More particularly, this disclosure relates to a method and a kit fir detecting a DNA fragment joining event or distinguishing an alternative splicing event. The present disclosure also relates to a method for administering a subject with proper treatment by steps of determining the risk of a particular cancer type or genotype.

公开(公告)号：US20240182951A1

公开(公告)日：2024-06-06

申请号：US18553167

申请日：2022-03-30

申请人： Jumpcode Genomics, Inc.

发明人： Keith BROWN ,  Jon ARMSTRONG ,  Azeem SIDDIQUE

IPC分类号： C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

CPC分类号： C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

摘要： Disclosed herein are compositions and methods related to the detection and/or elimination of a first nucleic acid and enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20240175093A1

公开(公告)日：2024-05-30

申请号：US18552414

申请日：2022-03-25

申请人： The University of Chicago ,  NorthShore University HealthSystem

发明人： Sean PITRODA ,  Mitchell POSNER ,  Ralph WEICHSELBAUM ,  Mark TALAMONTI

IPC分类号： C12Q1/6886 ,  C12Q1/686 ,  G16B25/10 ,  G16H50/20

CPC分类号： C12Q1/6886 ,  C12Q1/686 ,  G16B25/10 ,  G16H50/20 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/178

摘要： Methods, assays, and compositions for identifying molecular subtypes of metastatic cancer are disclosed. Methods include determining expression levels of genes and/or miRNAs in a sample of metastatic tissue and identifying the molecular subtype of the metastasis based on the determined expression levels using a neural network-based classifier. Methods may further include providing a prognosis and making treatment decision based on the molecular subtype of the metastasis.

公开(公告)号：US20240175075A1

公开(公告)日：2024-05-30

申请号：US18527656

申请日：2023-12-04

申请人： IntegenX Inc.

发明人： David Eberhart ,  William D. Nielsen ,  Helen Franklin ,  Stevan B. Jovanovich ,  Dennis Lehto ,  Kaiwan Chear ,  James Klevenberg ,  Chungsoo Charles Park ,  Corey M. Smith ,  Philip Justus Wunderle ,  David King

IPC分类号： C12Q1/6806 ,  B01L3/00 ,  C12Q1/686 ,  G01N27/447

CPC分类号： C12Q1/6806 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  C12Q1/686 ,  G01N27/44743 ,  G01N27/44791 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0848 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2400/0406 ,  B01L2400/0478

摘要： An instrument includes a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to close off a fluidic channel in the body. A cartridge interface is configured to engage the cartridge.

公开(公告)号：US20240167104A1

公开(公告)日：2024-05-23

申请号：US18505697

申请日：2023-11-09

申请人： NIPPN CORPORATION ,  FASMAC CO., LTD.

发明人： Mika TSUNA ,  Yoshitaka HARADA ,  Yasuhiro SETO ,  Kazuto TAKASAKI

IPC分类号： C12Q1/689 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N33/52

CPC分类号： C12Q1/689 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N33/52 ,  C12Q2600/156 ,  G01N2458/00 ,  G01N2469/00

摘要： Novel means that enables detection of the monocytogenes bacterium alone distinctly from other bacteria belonging to the genus Listeria with sufficiently high accuracy is disclosed. The present inventors intensively analyzed the genome of the monocytogenes bacterium to identify two genes (the lmo0084 gene and the lmo2736 gene) as target regions with which the monocytogenes bacterium can be specifically detected distinctly from other bacteria belonging to the genus Listeria utilizing a nucleic acid amplification method. By a further intensive study of the base sequences of these two genes, primer setting regions for highly accurate, specific detection of the monocytogenes bacterium alone were identified, and preferred particular examples of PCR primer sets, LAMP primer sets, and real-time PCR primer-probe sets were established.

公开(公告)号：US20240158778A1

公开(公告)日：2024-05-16

申请号：US18280653

申请日：2022-04-01

申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  THE UNIVERSITY OF CHICAGO

发明人： Georgi MARINOV ,  William J. GREENLEAF ,  Tong WU ,  Chuan HE

IPC分类号： C12N15/10 ,  C12Q1/686

CPC分类号： C12N15/102 ,  C12Q1/686

摘要： The disclosure pertains to a method for isolating DNA fragments that contain a binding site for a guide-RNA of an RNA-guided endonuclease. The method comprises contacting the RNA-guided endonuclease with a target double-stranded DNA, thereby producing a single stranded DNA (ss-DNA) at the site to which the guide-RNA binds. The ssDNA is then reacted with kethoxal or an analog thereof modified by a chemoselective group, thereby adding the chemoselective group to any unpaired guanine based within the ssDNA. A first binding member of a specific binding pair is linked to the chemoselective group, for example, via a cycloaddition reaction. The DNA is fragmented and the DNA fragments that contain the binding sites for the guide-RNA are enriched using a support that comprises a second binding member of the specific binding pair. The method can be used to identify binding sites for a guide-RNA thereby identifying off-target binding sites.

公开(公告)号：US11981954B2

公开(公告)日：2024-05-14

申请号：US17812380

申请日：2022-07-13

申请人： Roche Molecular Systems, Inc.

发明人： Kyle C. Cady ,  Brett Hanson ,  Paulino Abdon ,  Ryan Chan ,  Patrick Lin ,  Rochak Mehta ,  Troy Rabang

IPC分类号： C12P19/34 ,  C12Q1/18 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q1/6897 ,  C12Q1/6851 ,  C12Q1/686

CPC分类号： C12Q1/18 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q1/6897 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2561/101 ,  C12Q2561/113 ,  C12Q2565/1015 ,  C12Q2600/136

摘要： The present invention relates to compositions and methods for the use of polymerase chain reaction (PCR) as a reporter assay for rapid and simultaneous bacterial identification and phenotype testing for antimicrobial susceptibility (AST). The current invention uses a strategy that has shown the ability for multiplexing and for handling polymicrobial samples for antimicrobial susceptibility testing.

公开(公告)号：US20240150822A1

公开(公告)日：2024-05-09

申请号：US18386976

申请日：2023-11-03

申请人： Gen-Probe Incorporated

发明人： David BUSE ,  David Howard Combs ,  Norbert D. Hagen ,  David Opalsky ,  Bruce Richardson ,  Anita Frasad ,  Keith Moravick ,  Tyler Moore

IPC分类号： C12Q1/686 ,  B01L7/00 ,  G01N21/64

CPC分类号： C12Q1/686 ,  B01L7/52 ,  G01N21/6452 ,  B01L9/06 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2300/042 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/1822

摘要： An apparatus comprises a thermally conductive receptacle holder including a plurality of receptacle wells configured to receive receptacles, a plurality of optical fibers having first and second ends, wherein each first end is in optical communication with one of the receptacle wells and each second end is in optical communication with an excitation signal source and/or an emission signal detector, one or more thermal elements positioned proximal to the receptacle holder for altering the temperature of the receptacle holder, a heat sink in thermal communication with the receptacle holder, and one or more thermal devices disposed within the heat sink for pre-heating the heat sink.

公开(公告)号：US20240141421A1

公开(公告)日：2024-05-02

申请号：US18384971

申请日：2023-10-30

申请人： Stokes Bio Limited

发明人： Mark DAVIES ,  Tara DALTON

IPC分类号： C12Q1/686 ,  B01F25/10 ,  B01F33/30 ,  B01L3/00 ,  B01L7/00 ,  G01N35/08

CPC分类号： C12Q1/686 ,  B01F25/10 ,  B01F33/30 ,  B01L3/50273 ,  B01L3/502776 ,  B01L3/502784 ,  B01L7/525 ,  G01N35/08 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N35/1095

摘要： A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

公开(公告)号：US20240139746A1

公开(公告)日：2024-05-02

申请号：US18327194

申请日：2023-06-01

申请人： Kryptos Biotechnologies, Inc.

发明人： Austin So ,  Jun Ho Son

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/686

CPC分类号： B01L7/52 ,  B01L3/508 ,  C12Q1/686 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/1861

摘要： This disclosure describes various reaction vessel configurations that include a housing component; a reaction chamber defined by the housing component; and a light absorbing layer conforming to a portion of an interior-facing surface of the housing component that defines the reaction chamber, the light absorbing layer comprising multiple discrete regions. An energy source may direct light at one or more of the discrete regions of the light absorbing layer so as to heat the discrete regions and ultimately heat a solution within a reaction chamber.