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公开(公告)号：US11966086B2
公开(公告)日：2024-04-23
申请号：US17830115
申请日：2022-06-01
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
CPC classification number: G02B6/3668 , G01N21/253 , G01N21/6452 , G02B6/06 , G02B6/08 , G02B6/3672 , G02B6/4214 , G02B6/4246 , G02B6/4269 , G01N2021/6484
Abstract: A first time/temperature data set includes temperatures and time stamps recorded at time intervals during a first thermal cycling process and a time stamp for each time interval, and a second time/temperature data set includes temperatures and time stamps recorded at time intervals during a second thermal cycling process. Signal emissions at different signal detecting positions are sequentially measured at different time intervals to generate first and second time/signal emission data sets for receptacles subject to the first and second thermal cycling processes, respectively. Signal emissions of receptacles subject to the first thermal cycling process are synchronized with specific temperatures of the first thermal cycling process by comparing time stamps of the first time/signal emission data set for each receptacle with the time stamps of the first time/temperature data set that correspond with the specific temperature. Signal emissions of receptacles subject to the second thermal cycling process are synchronized with specific temperatures of the second thermal cycling process by comparing time stamps of the second time/signal emission data set for each receptacle with the time stamps of the second time/temperature data set that correspond with the specific temperature.
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公开(公告)号：US11840727B2
公开(公告)日：2023-12-12
申请号：US17028367
申请日：2020-09-22
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore , Byron J. Knight
CPC classification number: C12Q1/686 , B01L7/52 , G01N21/6452 , B01L9/06 , B01L2200/141 , B01L2200/142 , B01L2300/042 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/1822 , G01N2021/6482 , G01N2021/6484
Abstract: Multiple receptacles containing a reaction mixture are transported to corresponding receptacle wells of a thermally-conductive receptacle holder in thermal communication with a support that is in thermal communication with a heat sink. The heat sink is pre-heated to a temperature above ambient temperature, and the temperature of the receptacle holder is cycled. Optical communication is established between each of the receptacle wells and an excitation signal source and an emission signal detector during cycling, and whether an emission signal is emitted from any of the receptacles is determined as an indication of the presence of a target nucleic acid.
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公开(公告)号：US10563251B2
公开(公告)日：2020-02-18
申请号：US15951818
申请日：2018-04-12
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
Abstract: First and second sets of receptacles containing reagents for first and second amplification reactions, respectively, are placed in first and second receptacle holders, each associated with a thermal element. The receptacles are subjected to different first and second incubation processes resulting in the first and second amplification reactions in each of the first and second sets of receptacles that contain a first or a second target nucleic acid, respectively. The presence or absence of the first or second target nucleic acid, if any, is determined for each receptacle of the first and second sets of receptacles.
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公开(公告)号：US20180230517A1
公开(公告)日：2018-08-16
申请号：US15951818
申请日：2018-04-12
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
Abstract: First and second sets of receptacles containing reagents for first and second amplification reactions, respectively, are placed in first and second receptacle holders, each associated with a thermal element. The receptacles are subjected to different first and second incubation processes resulting in the first and second amplification reactions in each of the first and second sets of receptacles that contain a first or a second target nucleic acid, respectively. The presence or absence of the first or second target nucleic acid, if any, is determined for each receptacle of the first and second sets of receptacles.
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公开(公告)号：US20220162677A1
公开(公告)日：2022-05-26
申请号：US17668775
申请日：2022-02-10
Applicant: Gen-Probe Incorporated
Inventor： David A. Buse , David Howard Combs , Byron J. Knight , Norbert D. Hagen , David Opalsky , Jason F. Rhubottom , Keith Moravick , Tyler Moore , Anita Prasad , Bruce Richardson
IPC: C12Q1/6806 , G01N35/04 , B01L3/00 , B01L7/00 , C12Q1/686
Abstract: A method for performing a nucleic acid amplification assay employs a thermally-conductive receptacle holder with one or more receptacle wells, each conforming to an outer surface of a lower portion of a vial. A through-hole extends from an inner surface of each well to an outer surface of the holder. A thermal element is positioned proximal to the holder for altering a temperature of the holder. A signal detection module is configured to generate an excitation signal directed through the through-hole of the well and detect an optical emission from a fluid contained in the vial supported by the well. At least one well supports a capped vial containing a reagent for a nucleic acid amplification assay and including an opaque cap sealing an open end of the vial. The lower portion of the vial is contained within a well, and the cap is situated above a top surface of the holder.
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Patent Agency Ranking
公开(公告)号：US20210002704A1
公开(公告)日：2021-01-07
申请号：US17025735
申请日：2020-09-18
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore , Byron J. Knight
Abstract: Receptacles containing a reaction mixture are transported to receptacle wells of a thermally conductive receptacle holder, and the temperature of the holder is cycled with a thermoelectric device situated between a support and a first side of the holder while a first force is applied onto a second side of the holder. Optical communication between each well and an excitation signal source and an emission signal detector is established to determine whether an emission signal is emitted from any of the receptacles during temperature cycling as an indication of the presence of a target nucleic acid. The thermoelectric device may be situated between an upright portion of the support and the first side of the holder. A second force may be applied onto a top end of each receptacle in the holder by a cover moveable with respect to the receptacles between an open position and a closed position.
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公开(公告)号：US20190284614A1
公开(公告)日：2019-09-19
申请号：US16434910
申请日：2019-06-07
Applicant: Gen-Probe Incorporated
Inventor： David BUSE , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
Abstract: A thermally conductive receptacle holder includes a plurality of wells that each receive a receptacle. A cross-brace is mounted to a support to exert a force onto an outer surface of a compressive housing such that the compressive housing exerts a force onto the receptacle holder. An optical fiber associated with each well provides optical communication between the receptacle well and an excitation signal source and/or an emission signal detector.
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8.发明公开公开(公告)号：US20240150822A1
公开(公告)日：2024-05-09
申请号：US18386976
申请日：2023-11-03
Applicant: Gen-Probe Incorporated
Inventor： David BUSE , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Frasad , Keith Moravick , Tyler Moore
CPC classification number: C12Q1/686 , B01L7/52 , G01N21/6452 , B01L9/06 , B01L2200/141 , B01L2200/142 , B01L2300/042 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/1822
Abstract: An apparatus comprises a thermally conductive receptacle holder including a plurality of receptacle wells configured to receive receptacles, a plurality of optical fibers having first and second ends, wherein each first end is in optical communication with one of the receptacle wells and each second end is in optical communication with an excitation signal source and/or an emission signal detector, one or more thermal elements positioned proximal to the receptacle holder for altering the temperature of the receptacle holder, a heat sink in thermal communication with the receptacle holder, and one or more thermal devices disposed within the heat sink for pre-heating the heat sink.
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公开(公告)号：US11692220B2
公开(公告)日：2023-07-04
申请号：US17027433
申请日：2020-09-21
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore , Byron J. Knight
CPC classification number: C12Q1/686 , B01L7/52 , G01N21/6452 , B01L9/06 , B01L2200/141 , B01L2200/142 , B01L2300/042 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/1822 , G01N2021/6482 , G01N2021/6484
Abstract: An apparatus for performing nucleic acid amplification reactions includes a thermally-conductive receptacle holder with multiple receptacle wells. Each well has a through-hole extending from an inner surface of the well to an outer surface of the holder. A cover is rotatable between an open position and a closed position relative to the holder and is configured to exert a force onto any receptacles in the wells when the cover is in the closed position. The apparatus includes multiple optical fibers, and each of the optical fibers provides optical communication between one of the wells and an excitation signal source and/or an emission signal detector. A thermal element is positioned between a thermally-conductive support and the receptacle holder.
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10.发明授权公开(公告)号：US11230733B2
公开(公告)日：2022-01-25
申请号：US17025735
申请日：2020-09-18
Applicant: Gen-Probe Incorporated
Inventor： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore , Byron J. Knight
Abstract: Receptacles containing a reaction mixture are transported to receptacle wells of a thermally conductive receptacle holder, and the temperature of the holder is cycled with a thermoelectric device situated between a support and a first side of the holder while a first force is applied onto a second side of the holder. Optical communication between each well and an excitation signal source and an emission signal detector is established to determine whether an emission signal is emitted from any of the receptacles during temperature cycling as an indication of the presence of a target nucleic acid. The thermoelectric device may be situated between an upright portion of the support and the first side of the holder. A second force may be applied onto a top end of each receptacle in the holder by a cover moveable with respect to the receptacles between an open position and a closed position.
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