公开(公告)号：US20240141400A1

公开(公告)日：2024-05-02

申请号：US18494425

申请日：2023-10-25

申请人： Fenfei LENG

发明人： Fenfei LENG

IPC分类号： C12P19/34 ,  C12Q1/6844

CPC分类号： C12P19/34 ,  C12Q1/6846

摘要： A method, which synthesizes closed circular single-stranded and double-stranded DNA molecules using in vitro enzymatic systems, is described. Circular single-stranded DNA molecules and double-stranded DNA molecules (e.g., relaxed, or supercoiled) with various sizes can be synthesized. Unwanted DNA molecules, e.g., unligated oligomers, can be removed by exonucleases, such as T5 exonuclease, T7 exonuclease, lambda exonuclease, E. coli exonuclease I and/or III. A method of converting the single-stranded circular DNA molecules into double-stranded circular DNA molecules is also described. The single-stranded and double-stranded circular DNA molecules can be used in a variety of applications.

公开(公告)号：US11970787B2

公开(公告)日：2024-04-30

申请号：US17248722

申请日：2021-02-04

申请人： Life Technologies Corporation

发明人： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC分类号： C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/725

CPC分类号： C40B50/06 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C07K14/7051 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166

摘要： The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20240132948A1

公开(公告)日：2024-04-25

申请号：US18183418

申请日：2023-03-14

申请人： Pacific Biosciences of California, Inc.

发明人： Stephen YUE ,  Gene SHEN ,  Wei-Chuan (David) SUN

IPC分类号： C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/09 ,  C07K5/11 ,  C09B23/01 ,  C09B23/06 ,  C09B23/08 ,  C09B23/10 ,  C09B23/16 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/58

CPC分类号： C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/0815 ,  C07K5/1019 ,  C09B23/0025 ,  C09B23/0033 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/164 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/582 ,  G01N2333/9126

摘要： The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

公开(公告)号：US20240124924A1

公开(公告)日：2024-04-18

申请号：US18547258

申请日：2022-02-22

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE GENERAL HOSPITAL CORPORATION

发明人： Aziz Al'Khafaji ,  Frances Keer ,  Paul Blainey ,  Nir Hacohen

IPC分类号： C12Q1/6844 ,  C12Q1/6869

CPC分类号： C12Q1/6844 ,  C12Q1/6869

摘要： The present disclosure relates to methods and compositions for enhanced assessment of exogenous polynucleotide and/or polypeptide-mediated transcriptional perturbations at high throughput and single cell/droplet levels of resolution. In embodiments, nucleic acid fusions of exogenous polynucleotide(s) and associated target transcript(s) are produced within individually sequestered or discretely identifiable cells/lysates and analyzed for exogenous polynucleotide mediated perturbations across a vast population of droplets/cells within individual reactions. Kits for performance of the methods are also provided.

公开(公告)号：US20240124914A1

公开(公告)日：2024-04-18

申请号：US18476015

申请日：2023-09-27

申请人： Illumina, Inc.

发明人： Justin Robbins

IPC分类号： C12Q1/48 ,  C12Q1/6844 ,  C12Q1/6869

CPC分类号： C12Q1/485 ,  C12Q1/6844 ,  C12Q1/6869

摘要： This disclosure relates to novel thermophilic amplification compositions and methods, in particular for use in nucleic acid amplification and sequencing.

公开(公告)号：US20240117452A1

公开(公告)日：2024-04-11

申请号：US18270440

申请日：2022-01-05

申请人： Salus Discovery, LLC ,  Flambeau Diagnostics, LLC

发明人： Jay Warrick ,  Brianna Mullins ,  Patrick McMinn ,  Dave Beebe ,  Ryan Shogren ,  Mitch Geiger ,  Franklin Zhong ,  Duane S. Juang ,  Terry D. Juang

IPC分类号： C12Q1/70 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/6844

CPC分类号： C12Q1/701 ,  B01L3/0237 ,  B01L3/5085 ,  C12Q1/6844 ,  C12Q1/6806

摘要： Provided herein are systems and methods for isolating a target from a biological sample. In some aspects, provided herein are automated systems and methods for isolating a target from a plurality of biological samples. For example, provided herein are automated systems and methods for isolating viral nucleic acid from a biological sample using a magnetic force to draw the nucleic acid out of a liquid biological sample.

公开(公告)号：US11952630B2

公开(公告)日：2024-04-09

申请号：US17746400

申请日：2022-05-17

申请人： Gen-Probe Incorporated

发明人： Patrick Peterson ,  Tony Luu ,  Matthias Jost

IPC分类号： C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12Q1/6844 ,  C12Q2527/125 ,  C12Y207/07007

摘要： There is disclosed a composition of an aqueous solution comprising, consisting or consisting essentially of a flap endonuclease, a bulking agent and an organic buffer, wherein the aqueous solution has an inorganic salt concentration of 5 mM or less and wherein the composition is substantially free of glycerol.

公开(公告)号：US20240093274A1

公开(公告)日：2024-03-21

申请号：US18462936

申请日：2023-09-07

申请人： 10x Genomics Sweden AB

发明人： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg

IPC分类号： C12Q1/6837 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30 ,  G01N1/42 ,  G16B30/00 ,  G16B50/20 ,  G16B50/30

CPC分类号： C12Q1/6837 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30 ,  G01N1/42 ,  G16B30/00 ,  G16B50/20 ,  G16B50/30 ,  C12Y600/00

摘要： Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20240084404A1

公开(公告)日：2024-03-14

申请号：US18269171

申请日：2021-12-23

申请人： Roche Molecular Systems, Inc.

发明人： Rajiv Dua ,  Slav Dugenny ,  Xiaoyun Zhang

IPC分类号： C12Q1/689 ,  C12Q1/6844

CPC分类号： C12Q1/689 ,  C12Q1/6844 ,  C12Q1/6806

摘要： The present invention relates to methods for the specific lysis of eukaryotic cells in a sample containing microorganisms such as bacterium and fungus by incubating the sample in a buffer of around pH 9 that contains a non-ionic detergent.

公开(公告)号：US20240076725A1

公开(公告)日：2024-03-07

申请号：US18262397

申请日：2022-05-10

申请人： BEIJING BOE TECHNOLOGY DEVELOPMENT CO., LTD. ,  BOE TECHNOLOGY GROUP CO., LTD.

发明人： Zhukai Liu ,  Ruijun Deng ,  Kang Peng ,  Ding Ding

IPC分类号： C12Q1/6844 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/6844 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/1827

摘要： The present disclosure provides a microfluidic substrate, a microfluidic chip, a method for preparing the microfluidic chip, and a method for using the microfluidic chip. The microfluidic substrate includes a substrate including a plurality of microcavity regions arranged m an array, each of the plurality of microcavity regions includes a first portion and a second portion that are stacked, and the depth of the first portion is x, and the first portion includes a top opening that is circular in shape and has a diameter D, the relationship between the diameter D of the top opening and the depth x is approximately D=2x+y, where the range of x is from 20 microns to 400 microns, and the range of y is from 5 microns to 30 microns.