公开(公告)号：US11708590B2

公开(公告)日：2023-07-25

申请号：US16631232

申请日：2018-07-16

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： David Nielsen ,  Brian Thompson

IPC分类号： C12P7/18 ,  C12P7/46 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12P7/22

CPC分类号： C12P7/46 ,  C12N9/0069 ,  C12N9/0073 ,  C12N9/88 ,  C12N9/90 ,  C12P7/22 ,  C12Y113/11001 ,  C12Y114/13002 ,  C12Y114/13007 ,  C12Y401/01061 ,  C12Y401/01063 ,  C12Y401/01091 ,  C12Y401/0304 ,  C12Y401/99002 ,  C12Y402/0101 ,  C12Y402/99021 ,  C12Y504/04002

摘要： Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.

公开(公告)号：US20230227799A1

公开(公告)日：2023-07-20

申请号：US18047589

申请日：2022-10-18

申请人： Oxford Nanopore Technologies PLC

发明人： Andrew John Heron ,  James Anthony Clarke ,  Ruth Moysey ,  Elizabeth Jayne Wallace ,  Mark John Bruce ,  Lakmal Jayasinghe ,  Domenico Caprotti ,  Szabolcs Soeroes ,  Luke McNeill ,  David Antoni Alves ,  Rebecca Victoria Bowen ,  John Milton

IPC分类号： C12N9/14 ,  C12N9/90 ,  C12Q1/6827

CPC分类号： C12N9/14 ,  C12Y306/04012 ,  C12N9/90 ,  C12Y599/01003 ,  C12Y599/01002 ,  C12Q1/6827 ,  C07K2319/80

摘要： The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

公开(公告)号：US20230212624A1

公开(公告)日：2023-07-06

申请号：US18018360

申请日：2021-07-29

申请人： Givaudan SA

发明人： Jean-Pierre BARRAS ,  Sylvie MOREL ,  Eric EICHHORN

IPC分类号： C12P17/04 ,  C12N9/90

CPC分类号： C12P17/04 ,  C12N9/90 ,  C12Y504/99017

摘要： A method for purifying a crude flavor or perfumery or cosmetic ingredient or intermediate, for example crude (−)-Ambrox, comprising a number of washing steps, the products of said method, and uses of said product.

公开(公告)号：US20230212254A1

公开(公告)日：2023-07-06

申请号：US17904221

申请日：2021-02-15

申请人： BEIJING YONGTAI RUIKE BIOTECHNOLOGY COMPANY LTD ,  PHAROS VACCINE INC.

发明人： Hoeon KIM ,  Choonju JEON ,  Yu WANG ,  Namchul JUNG

IPC分类号： C07K14/725 ,  C12N5/0783 ,  C12N15/86 ,  C12N9/90 ,  A61K35/17 ,  C07K14/705 ,  C07K14/435 ,  C07K14/005 ,  C07K14/47 ,  A61P35/02

CPC分类号： C07K14/7051 ,  A61K35/17 ,  A61P35/02 ,  C07K14/005 ,  C07K14/435 ,  C07K14/4748 ,  C07K14/70503 ,  C07K14/70517 ,  C07K14/70578 ,  C07K14/70596 ,  C12N5/0636 ,  C12N9/90 ,  C12N15/86 ,  C07K2317/53 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/72 ,  C12N2740/15043 ,  C12N2740/15071

摘要： The present invention relates to an immune cell that are engineered to overexpress cell signaling pathway modulator(s) and a use thereof. As a specific example, an immune cell expressing a fusion protein comprising a chimeric antigen receptor and a cell signaling pathway modulator(s) performs an immune response by selecting a target cancer cell by a chimeric antigen receptor expressed on a cell membrane. In this case, the cell signaling pathway modulator is overexpressed in the cytoplasm, thereby being capable of regulating the activity of an immune cell. Therefore, the fusion protein comprising a chimeric antigen receptor and cell signaling pathway modulator(s), and the immune cell engineered to overexpress the cell signaling pathway modulator(s) of the present invention can be usefully used in the treatment of cancer.

公开(公告)号：US20230183671A1

公开(公告)日：2023-06-15

申请号：US17926032

申请日：2021-06-04

申请人： TOHOKU UNIVERSITY ,  Tokushima University

发明人： Masaki OKUMURA ,  Motonori MATSUSAKI ,  Kenji INABA ,  Tomohide SAIO ,  Shingo KANEMURA

IPC分类号： C12N9/90 ,  C07K1/10

CPC分类号： C12N9/90 ,  C07K1/10 ,  C12Y503/04001

摘要： An object to be achieved by the present in is to prepare a droplet using a protein that has not been known heretofore to form a droplet. The present invention provides a droplet including an assembly of a protein, wherein the protein is a PDI family protein and/or an artificial protein, and contains a thioredoxin-like domain.

公开(公告)号：US20230175027A1

公开(公告)日：2023-06-08

申请号：US17995512

申请日：2021-04-14

申请人： Givaudan SA

发明人： Eric EICHHORN ,  Felix FLACHSMANN ,  Andreas GOEKE

IPC分类号： C12P17/18 ,  C12N9/90 ,  C11B9/00

CPC分类号： C12P17/181 ,  C12N9/90 ,  C12Y504/99017 ,  C11B9/008

摘要： An enzyme-mediated method for the production of Amberketal and Amberketal homologues, the products of said method, and uses of said products.

公开(公告)号：US20230174960A1

公开(公告)日：2023-06-08

申请号：US17926208

申请日：2021-05-20

申请人： MEIOGENIX ,  INSTITUT CURIE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  SORBONNE UNIVERSITE

发明人： ALEXANDRE SERERO ,  ALAIN NICOLAS

IPC分类号： C12N9/22 ,  C12N9/90 ,  C12N15/63

CPC分类号： C12N9/22 ,  C12N9/90 ,  C12N15/63 ,  C12N2310/20

摘要： The present invention relates to a fusion protein comprising a domain of a nuclease from the class II CRISPR system, and a Spo11 domain, wherein both domains of the fusion protein have deficient nuclease activity, as well as the use of this protein to induce targeted meiotic recombinations in a eukaryotic cell.

公开(公告)号：US20230159941A1

公开(公告)日：2023-05-25

申请号：US17297727

申请日：2020-01-13

申请人： Clariant Produkte (Deutschland) GmbH

发明人： Heiko DIETZ ,  Magdalena MERTEL ,  Jörg CLAREN ,  Alexander FARWICK

IPC分类号： C12N15/81 ,  C12N9/90

CPC分类号： C12N15/81 ,  C12N9/90

摘要： The present invention comprises a chimeric promoter which initiates the transcription of a gene depending on different conditions such as carbon sources. Further the invention relates to a recombinant DNA fragment comprising the chimeric promoter, an expression plasmid comprising the recombinant DNA fragment and a host cell transformed with the recombinant DNA fragment.

公开(公告)号：US20190211342A1

公开(公告)日：2019-07-11

申请号：US16362897

申请日：2019-03-25

申请人： Yeda Research and Development Co. Ltd.

发明人： Ron MILO ,  Niv ANTONOVSKY ,  Elad NOOR ,  Arren BAR-EVEN ,  Yehudit ZOHAR ,  Lior ZELCBUCH ,  Shmuel GLEIZER ,  Shira AMRAM

IPC分类号： C12N15/70 ,  C07K14/195 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C07K14/245 ,  C12N1/20

CPC分类号： C12N15/70 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/88 ,  C12N9/90 ,  C12Y101/01049 ,  C12Y203/03009 ,  C12Y207/01011 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y402/01011 ,  C12Y504/02

摘要： A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.

公开(公告)号：US20190194699A1

公开(公告)日：2019-06-27

申请号：US16230932

申请日：2018-12-21

申请人： Centrome, Inc. dba Advanced Biotech Inc.

发明人： Eduardo Esteban Agosin Trumper ,  Francisco Javier Saitua Perez ,  Javiera Cecilia Lopez Salinas ,  Maciej E Domaradzki ,  Maximiliano Ibaceta Acevedo ,  Natalia Arenas Frigolett

IPC分类号： C12P7/26 ,  C12N15/81 ,  C12N9/10

CPC分类号： C12P7/26 ,  C12N9/0069 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/81 ,  C12Y113/11071 ,  C12Y205/01029 ,  C12Y505/01018

摘要： This invention provides improved biological synthesis of the apocarotenoid α-ionone in Saccharomyces cerevisiae. The final native step involved in the natural apocarotenoid pathway depends on an endogenous farnesyl pyrophosphate synthase (FPPs). From there, heterologous geranylgeranyl pyrophosphate synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtI), lycopene ε-cyclase (LycE) and a Carotenoid Cleavage Dioxygenase (CCD1) are required to complete the synthesis of α-ionone. Lycopene ε-cyclase from lettuce (Lactuca sativa) or modified cyclase from Arabidopsis thaliana was used to overproduce lycopene which was then cleaved by the carotenoid cleavage dioxygenase from Petunia hybrida (Ph-CCD1).