公开(公告)号：US11708590B2

公开(公告)日：2023-07-25

申请号：US16631232

申请日：2018-07-16

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： David Nielsen ,  Brian Thompson

IPC分类号： C12P7/18 ,  C12P7/46 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12P7/22

CPC分类号： C12P7/46 ,  C12N9/0069 ,  C12N9/0073 ,  C12N9/88 ,  C12N9/90 ,  C12P7/22 ,  C12Y113/11001 ,  C12Y114/13002 ,  C12Y114/13007 ,  C12Y401/01061 ,  C12Y401/01063 ,  C12Y401/01091 ,  C12Y401/0304 ,  C12Y401/99002 ,  C12Y402/0101 ,  C12Y402/99021 ,  C12Y504/04002

摘要： Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.