公开(公告)号：US20170067063A1

公开(公告)日：2017-03-09

申请号：US15123198

申请日：2015-03-06

申请人： EVOLVA SA

发明人： A.S. Sathish Kumar

IPC分类号： C12N15/52 ,  C12N15/81 ,  C12N9/10 ,  C12P7/24 ,  C12N9/02

CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0069 ,  C12N9/1085 ,  C12N15/81 ,  C12P7/24 ,  C12P7/44 ,  C12Y102/01003 ,  C12Y103/99 ,  C12Y113/11071 ,  C12Y205/01 ,  C12Y205/01029

摘要： Recombinant microorganisms and methods for producing saffron compounds including crocetin, crocetin dialdehyde, crocin or picrocrocin are disclosed herein.

摘要翻译： 本文公开了重组微生物和用于生产藏红花素化合物的方法，所述方法包括番茄红素，番红花素二醛，西红花素或微生物素。

公开(公告)号：US20190194699A1

公开(公告)日：2019-06-27

申请号：US16230932

申请日：2018-12-21

申请人： Centrome, Inc. dba Advanced Biotech Inc.

发明人： Eduardo Esteban Agosin Trumper ,  Francisco Javier Saitua Perez ,  Javiera Cecilia Lopez Salinas ,  Maciej E Domaradzki ,  Maximiliano Ibaceta Acevedo ,  Natalia Arenas Frigolett

IPC分类号： C12P7/26 ,  C12N15/81 ,  C12N9/10

CPC分类号： C12P7/26 ,  C12N9/0069 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/81 ,  C12Y113/11071 ,  C12Y205/01029 ,  C12Y505/01018

摘要： This invention provides improved biological synthesis of the apocarotenoid α-ionone in Saccharomyces cerevisiae. The final native step involved in the natural apocarotenoid pathway depends on an endogenous farnesyl pyrophosphate synthase (FPPs). From there, heterologous geranylgeranyl pyrophosphate synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtI), lycopene ε-cyclase (LycE) and a Carotenoid Cleavage Dioxygenase (CCD1) are required to complete the synthesis of α-ionone. Lycopene ε-cyclase from lettuce (Lactuca sativa) or modified cyclase from Arabidopsis thaliana was used to overproduce lycopene which was then cleaved by the carotenoid cleavage dioxygenase from Petunia hybrida (Ph-CCD1).