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46 条记录 (耗时 0.017 秒)

    Process and reagent for the determination of creatinine
    31.
    发明授权
    Process and reagent for the determination of creatinine 失效
    用于测定肌酐的方法和试剂

    公开(公告)号：US4543325A

    公开(公告)日：1985-09-24

    申请号：US449925

    申请日：1982-12-15

    申请人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    发明人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

    IPC分类号： G01N33/53 ,  G01N33/70 ,  C12N9/96 ,  C12Q1/34

    CPC分类号： G01N33/70 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S436/822

    摘要： The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured.The present invention also provides a reagent for the immunological determination of creatinine, wherein it contains creatinine iminohydrolase, antibodies against a conjugate of a hydantoin of the general formula: ##STR2## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance, a conjugate of a hydantoin of general formula (I) with a second hapten carrier substance which does not cross-react substantially with the first hapten carrier substance and a buffer substance.

    摘要翻译： 本发明提供了一种免疫学测定肌酐的方法，其中将肌酐转化为1-甲基乙内酰脲，所形成的1-甲基乙内酰脲在水性介质中与针对通式 ：其中可以相同或不同的R 1，R 2，R 3和R 4为氢原子，含有至多3个碳原子的烷基或苯基，其中第一半抗原载体物质适用于抗体 与通式（I）的第二乙内酰脲衍生物与第二半抗原载体物质的缀合物反应，抗体和缀合物之一是固相或溶解形式存在，另一种成分以溶解形式存在 并且测量了与第二半抗原载体物质的抗体和乙内酰脲缀合物之间的结合反应的抑制。 本发明还提供了一种用于免疫测定肌酐的试剂，其中含有肌酐亚氨基水解酶，针对通式如下的乙内酰脲的缀合物的抗体：其中R1，R2，R3和R4可以相同 或不同的是氢原子，含有至多3个碳原子的烷基或苯基，具有第一半抗原载体物质，通式（I）的乙内酰脲与不会基本上交叉反应的第二半抗原载体物质的缀合物 具有第一半抗原载体物质和缓冲物质。

    1-(Substituted phenyl) aminoantipyrin compounds
    33.
    发明授权
    1-(Substituted phenyl) aminoantipyrin compounds 失效
    1-（取代苯基）氨基鸟嘌呤化合物

    公开(公告)号：US4394512A

    公开(公告)日：1983-07-19

    申请号：US228011

    申请日：1981-01-23

    申请人： Hans-Georg Batz

    发明人： Hans-Georg Batz

    IPC分类号： G01N33/48 ,  C07D231/26 ,  C07D231/46 ,  C12Q1/28

    CPC分类号： C07D231/26 ,  C07D231/46 ,  C12Q1/28 ,  C12Q2326/00 ,  C12Q2326/50 ,  C12Q2326/96 ,  Y10S436/904 ,  Y10T436/206664

    摘要： Compounds of the general formula ##STR1## wherein R represents an alkyl group of 1 to 3 carbon atoms or the group N(R.sub.2).sub.2, in which R.sub.2 represents a hydrogen atom, an alkyl group of 1 to 3 carbon atoms, or an acyl group of 1 to 3 carbon atoms, and R.sub.1 has the same meaning as R or is a hydrogen atom, are new and are suitable as chromogens for measuring H.sub.2 O.sub.2 formation in enzymatic reactions.

    摘要翻译： 其中R表示1至3个碳原子的烷基或基团N（R 2）2的化合物，其中R 2表示氢原子，1至3个碳原子的烷基或酰基 1至3个碳原子的基团，并且R 1具有与R相同的含义或氢原子，是新的并且适合作为用于测量酶反应中的H 2 O 2形成的色原体。

    Resorufin derivatives
    37.
    发明授权
    Resorufin derivatives 失效
    Resorufin衍生物

    公开(公告)号：US5304645A

    公开(公告)日：1994-04-19

    申请号：US758288

    申请日：1991-03-28

    申请人： Christian Klein ,  Hans-Georg Batz ,  Rupert Herrmann

    发明人： Christian Klein ,  Hans-Georg Batz ,  Rupert Herrmann

    IPC分类号： C07C237/22 ,  C07C279/10 ,  C07D265/38 ,  C07D413/12 ,  C07J71/00 ,  C07K1/113 ,  C07K14/00 ,  C07K14/42 ,  C07K14/575 ,  C07K14/705 ,  C07K16/00 ,  C07K19/00 ,  C09B19/00 ,  G01N33/533 ,  A61K31/435 ,  A61K31/495

    CPC分类号： G01N33/533 ,  C07D265/38 ,  Y10S436/80 ,  Y10S530/802

    摘要： The present invention provides resorufin derivatives of the general formulae: ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.5, which can be the same or different, are hydrogen, halogen, carboxyl, carboxamido, lower alkoxycarbonyl, cyano or nitro groups or lower alkyl or lower alkoxy radicals, which can be substituted by carboxyl, carboxamido, lower alkoxycarbonyl, cyano or nitro groups, and wherein R.sup.4 and R.sup.5 can together also represent an anellated aromatic residue, Z is a bridge member, A is the residue of a ligand and n is a whole number of from 1 to 200.The present invention also provides processes for the preparation of these resorufin derivatives, as well as intermediates for the preparation thereof.

    摘要翻译： 本发明提供通式为：其中R 1，R 2，R 3，R 4和R 5可以相同或不同的氢，卤素，羧基，甲酰胺基，低级烷氧基羰基，氰基或硝基的通式 低级烷基或低级烷氧基，其可以被羧基，甲酰氨基，低级烷氧基羰基，氰基或硝基取代，并且其中R 4和R 5一起也可以代表苯并芳族残基，Z是桥连，A是 配体，n是1至200的整数。本发明还提供制备这些稀释剂衍生物的方法，以及制备它们的中间体。

    Use of aniline derivates as coupling components in oxidative color formation reactions
    39.
    发明授权
    Use of aniline derivates as coupling components in oxidative color formation reactions 失效
    在氧化色彩形成反应中苯胺衍生物作为偶联组分的使用

    公开(公告)号：US4845030A

    公开(公告)日：1989-07-04

    申请号：US122600

    申请日：1987-11-20

    申请人： Hans-Georg Batz ,  Rupert Herrmann ,  Fritz Topfmeier ,  Helmut Schlumberger

    发明人： Hans-Georg Batz ,  Rupert Herrmann ,  Fritz Topfmeier ,  Helmut Schlumberger

    IPC分类号： G01N33/52 ,  C09B57/00 ,  C12Q1/28 ,  G01N21/77 ,  G01N21/78 ,  G01N33/50

    CPC分类号： C12Q1/28 ,  C12Q2326/00 ,  Y10T436/144444 ,  Y10T436/206664

    摘要： The present invention is concerned with the use of aniline derivatives of the general formula: ##STR1## wherein R.sup.1 is a hydrogen atom or a --(CH.sub.2).sub.n --X radical, in which n is a whole number of from 1 to 3, X is H, OH, NH.sub.2, CH.sub.3 CONH, CH.sub.3 SO.sub.2 NH, COOH, SO.sub.3 H or --ArSO.sub.3 H, Ar being an optionally substituted arylene radical, m is a whole number of from 2 to 4, R.sup.3 is a hydrogen atom, an alkyl radical containing up to 3 carbon atoms, OCH.sub.3, CH.sub.3 CONH, COOH or SO.sub.3 H and R.sup.4 is a hydrogen, chlorine or bromine atom or a carboxylic acid or sulphonic acid group, as coupling components in oxidative color formation reactions.The present invention also provides an agent for the analytical determination of oxidizing substances by oxidative color coupling which, as coupling component, contains at least one compound of general formula (I).

    摘要翻译： 本发明涉及以下通式的苯胺衍生物的用途：其中R 1为氢原子或 - （CH 2）n X基团，其中n为1至3的整数， X是H，OH，NH 2，CH 3 CONH，CH 3 SO 2 NH，COOH，SO 3 H或-ArSO 3 H，Ar是任选取代的亚芳基，m是整数2至4，R 3是氢原子， 3个碳原子，OCH 3，CH 3 CONH，COOH或SO 3 H，R 4是氢，氯或溴原子或羧酸或磺酸基团，作为氧化颜色形成反应中的偶联组分。 本发明还提供了一种通过氧化颜色偶联分析测定氧化物质的试剂，其作为偶联组分含有至少一种通式（I）的化合物。

    Process and analytical agent for the determination of the activity of glutamate-oxalacetate-transaminase
    40.
    发明授权
    Process and analytical agent for the determination of the activity of glutamate-oxalacetate-transaminase 失效
    用于测定谷氨酸 - 乙酸肝素 - 转氨酶活性的方法和分析试剂

    公开(公告)号：US4591553A

    公开(公告)日：1986-05-27

    申请号：US500111

    申请日：1983-06-01

    申请人： Ulfert Deneke ,  Wolfgang Gruber ,  Hans-Georg Batz

    发明人： Ulfert Deneke ,  Wolfgang Gruber ,  Hans-Georg Batz

    IPC分类号： G01N33/52 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/48 ,  C12Q1/52

    CPC分类号： C12Q1/52 ,  Y10S435/805

    摘要： The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which(a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalyzed by GOT, to give pyruvate, sulphur dioxide and glutamate,(b) the resultant pyruvate is reacted with phosphate and oxygen, catalyzed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen peroxide,(c) the hydrogen peroxide and a chromogen is reacted, catalyzed by a peroxidase, to give a dyestuff and water and(d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole) or a related substance.The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic acid, a chromogen, peroxidase, pyruvate oxidase, soluble phosphate, buffer and water, wherein the chromogen is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA) or 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole).

    摘要翻译： 本发明提供了一种测定谷氨酸酰乙酰转氨酶（GOT）在水溶液中的活性的方法，其中（a）α-酮戊二酸与由GOT催化的丙氨酸 - 亚磺酸反应得到丙酮酸，二氧化硫和谷氨酸 ，（b）所得丙酮酸盐与磷酸盐和氧气反应，由丙酮酸氧化酶催化，得到乙酰磷酸盐，二氧化碳和过氧化氢，（c）过氧化氢和色原反应，由过氧化物酶催化，得到 染料和水，（d）确定形成的染料的量，其中使用的色原是N-（3'-磺酰基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA） 5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] - 咪唑（B-DDH-咪唑）或相关物质。 本发明还提供了用于测定GOT的活性的分析剂，其包括α-酮戊二酸，丙氨酸 - 亚磺酸，色原，过氧化物酶，丙酮酸氧化酶，可溶性磷酸盐，缓冲液和水，其中色原为N-（3 4-磺基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA）或4,5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] 咪唑（B-DDH-咪唑）。