公开(公告)号：US4591553A

公开(公告)日：1986-05-27

申请号：US500111

申请日：1983-06-01

申请人： Ulfert Deneke ,  Wolfgang Gruber ,  Hans-Georg Batz

发明人： Ulfert Deneke ,  Wolfgang Gruber ,  Hans-Georg Batz

IPC分类号： G01N33/52 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/48 ,  C12Q1/52

CPC分类号： C12Q1/52 ,  Y10S435/805

摘要： The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which(a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalyzed by GOT, to give pyruvate, sulphur dioxide and glutamate,(b) the resultant pyruvate is reacted with phosphate and oxygen, catalyzed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen peroxide,(c) the hydrogen peroxide and a chromogen is reacted, catalyzed by a peroxidase, to give a dyestuff and water and(d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole) or a related substance.The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic acid, a chromogen, peroxidase, pyruvate oxidase, soluble phosphate, buffer and water, wherein the chromogen is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA) or 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole).

摘要翻译： 本发明提供了一种测定谷氨酸酰乙酰转氨酶（GOT）在水溶液中的活性的方法，其中（a）α-酮戊二酸与由GOT催化的丙氨酸 - 亚磺酸反应得到丙酮酸，二氧化硫和谷氨酸 ，（b）所得丙酮酸盐与磷酸盐和氧气反应，由丙酮酸氧化酶催化，得到乙酰磷酸盐，二氧化碳和过氧化氢，（c）过氧化氢和色原反应，由过氧化物酶催化，得到 染料和水，（d）确定形成的染料的量，其中使用的色原是N-（3'-磺酰基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA） 5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] - 咪唑（B-DDH-咪唑）或相关物质。 本发明还提供了用于测定GOT的活性的分析剂，其包括α-酮戊二酸，丙氨酸 - 亚磺酸，色原，过氧化物酶，丙酮酸氧化酶，可溶性磷酸盐，缓冲液和水，其中色原为N-（3 4-磺基-4'-羟基-5'-氯苯基）-4-氨基安替比林（SHYC-AA）或4,5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] 咪唑（B-DDH-咪唑）。

公开(公告)号：US4544631A

公开(公告)日：1985-10-01

申请号：US311856

申请日：1981-10-16

申请人： Elli Rauscher ,  Ulrich Neumann ,  August W. Wahlefeld ,  Alexander Hagen ,  Wolfgang Gruber ,  Joachim Ziegenhorn ,  Eugen Schaich ,  Ulfert Deneke ,  Gerhard Michal ,  Gunter Weimann

发明人： Elli Rauscher ,  Ulrich Neumann ,  August W. Wahlefeld ,  Alexander Hagen ,  Wolfgang Gruber ,  Joachim Ziegenhorn ,  Eugen Schaich ,  Ulfert Deneke ,  Gerhard Michal ,  Gunter Weimann

IPC分类号： G01N33/50 ,  C07H15/04 ,  C07H15/203 ,  C12Q1/40 ,  C12Q1/54 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/48

CPC分类号： C12Q1/40 ,  C12Q2334/10 ,  Y10S435/805 ,  Y10S435/81

摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.

摘要翻译： α-淀粉酶通过α-淀粉酶底物的酶裂解和裂变产物的测定来确定，其中用作底物的麦芽麦角糖化合物，其中R是葡萄糖苷，苯基葡糖苷，单硝基苯基葡糖苷，二硝基苯基葡糖苷， 山梨糖醇或葡萄糖酸基团。 还提供了包含这种底物的试剂和用于通过α-淀粉酶测定由淀粉酶底物形成的裂变产物的系统。

公开(公告)号：US5108913A

公开(公告)日：1992-04-28

申请号：US545092

申请日：1990-06-27

申请人： Elli Rauscher ,  Ulrich Neumann ,  August W. Wahlefeld ,  Alexander Hagen ,  Wolfgang Gruber ,  Joachim Ziegenhorn ,  Eugen Schaich ,  Ulfert Deneke ,  Michal Gerhard ,  Gunter Weimann

发明人： Elli Rauscher ,  Ulrich Neumann ,  August W. Wahlefeld ,  Alexander Hagen ,  Wolfgang Gruber ,  Joachim Ziegenhorn ,  Eugen Schaich ,  Ulfert Deneke ,  Michal Gerhard ,  Gunter Weimann

IPC分类号： G01N33/50 ,  C07H15/04 ,  C07H15/203 ,  C12Q1/40

CPC分类号： C12Q1/40 ,  C12Q2334/10 ,  Y10S435/805 ,  Y10S435/81

摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.

公开(公告)号：US4337310A

公开(公告)日：1982-06-29

申请号：US184675

申请日：1980-09-08

申请人： Hans-Georg Batz ,  Elli Rauscher ,  Gunter Weimann ,  August W. Wahlefeld ,  Wolfgang Gruber

发明人： Hans-Georg Batz ,  Elli Rauscher ,  Gunter Weimann ,  August W. Wahlefeld ,  Wolfgang Gruber

IPC分类号： G01N33/50 ,  C08B31/00 ,  C08B33/00 ,  C12Q1/40

CPC分类号： C08B31/003 ,  C12Q1/40

摘要： Method for determining .alpha.-amylase by incubating with a starch substrate, wherein the substrate is based upon particulate starch derivatized with detectable groups for the determination of .alpha.-amylase, the substrate consisting of superficially cross-linked starch or amylose grains having a regulated, reduced swellability, said grains having a diameter of from 0.01 to 0.20 mm; these grains are prepared by suspending non-swollen grains in a solution with only enough cross-linking agent to achieve superficial cross-linking and then derivatizing the grains with the detectable groups for the determination of .alpha.-amylase.

摘要翻译： 通过与淀粉底物孵育来测定α-淀粉酶的方法，其中所述底物是基于用可检测的基团衍生的用于测定α-淀粉酶的颗粒淀粉，所述底物由表面交联的淀粉或直链淀粉颗粒组成，所述淀粉或直链淀粉颗粒具有调节的， 所述颗粒具有0.01至0.20mm的直径; 这些颗粒通过将非溶胀颗粒悬浮在仅具有足够交联剂的溶液中以实现表面交联，然后用可检测的基团衍生化以测定α-淀粉酶来制备。

公开(公告)号：US4184848A

公开(公告)日：1980-01-22

申请号：US836111

申请日：1977-09-23

申请人： Hans-Georg Batz ,  Brigitte Draeger ,  August W. Wahlefeld ,  Gunter Weimann ,  Wolfgang Gruber

发明人： Hans-Georg Batz ,  Brigitte Draeger ,  August W. Wahlefeld ,  Gunter Weimann ,  Wolfgang Gruber

IPC分类号： C07C69/28 ,  C12Q1/00 ,  G01N21/82 ,  G01N33/48 ,  G01N33/50 ,  G01N33/52 ,  G01N33/16 ,  G01N31/14

CPC分类号： C12Q1/00 ,  G01N33/50 ,  Y10T436/25125

摘要： An agent for the elimination of turbidity in serum, especially in serum samples wherein photometric measurements are to be made, consisting essentially of one or more mono- or di-esters of polyethylene glycol and fatty acids of 9 to 14 carbon atoms with at most 8 ethylene oxide units per fatty acid moiety in the molecule, said esters having an HLB value of from 8 to 12.8.

摘要翻译： 用于消除血清中浊度的试剂，特别是在其中进行光度测量的血清样品中，基本上由一种或多种聚乙二醇和9至14个碳原子的脂肪酸的单酯或二酯组成，最多8个 分子中每个脂肪酸部分的环氧乙烷单元，所述酯的HLB值为8至12.8。

公开(公告)号：US08105874B2

公开(公告)日：2012-01-31

申请号：US12258742

申请日：2008-10-27

申请人： Wolfgang Gruber ,  Ronald Kakoschke ,  Thomas Schweizer ,  Dominik Wegertseder

发明人： Wolfgang Gruber ,  Ronald Kakoschke ,  Thomas Schweizer ,  Dominik Wegertseder

IPC分类号： H01L21/00

CPC分类号： G11C5/025 ,  H01L25/18 ,  H01L2924/0002 ,  H01L2924/00

摘要： A memory circuit arrangement and fabrication method thereof are presented in which the parts of the memory circuit arrangement are situated on two different substrates. An integrated memory cell array is situated on one substrate. An integrated control circuit that controls access to the memory cells is situated on the other (logic circuit) substrate. The control circuit controls sequences when reading, writing or erasing content of a memory cell. The logic circuit substrate also contains a CPU and encryption coprocessor. The memory circuit contains a sense amplifier, with the aid of which the memory state of a memory cell can be determined, and a decoding circuit that selects a word or bit line.

摘要翻译： 提出了一种存储器电路装置及其制造方法，其中存储器电路装置的部件位于两个不同的衬底上。 集成存储单元阵列位于一个衬底上。 控制对存储单元的访问的集成控制电路位于另一（逻辑电路）基板上。 当读取，写入或擦除存储单元的内容时，控制电路控制序列。 逻辑电路基板还包含CPU和加密协处理器。 存储器电路包括读出放大器，借助于此可以确定存储器单元的存储状态，以及选择字或位线的解码电路。

公开(公告)号：US08004869B2

公开(公告)日：2011-08-23

申请号：US12772590

申请日：2010-05-03

申请人： Wolfgang Gruber ,  Ronald Kakoschke ,  Thomas Schweizer ,  Dominik Wegertseder

发明人： Wolfgang Gruber ,  Ronald Kakoschke ,  Thomas Schweizer ,  Dominik Wegertseder

IPC分类号： G11C5/06 ,  G11C5/02 ,  H01L21/00

CPC分类号： G11C5/025 ,  H01L25/18 ,  H01L2924/0002 ,  H01L2924/00

摘要： A memory circuit arrangement and fabrication method thereof are presented in which the parts of the memory circuit arrangement are situated on two different substrates. An integrated memory cell array is situated on one substrate. An integrated control circuit that controls access to the memory cells is situated on the other (logic circuit) substrate. The control circuit controls sequences when reading, writing or erasing content of a memory cell. The logic circuit substrate also contains a CPU and encryption coprocessor. The memory circuit contains a sense amplifier, with the aid of which the memory state of a memory cell can be determined, and a decoding circuit that selects a word or bit line.

摘要翻译： 提出了一种存储器电路装置及其制造方法，其中存储器电路装置的部件位于两个不同的衬底上。 集成存储单元阵列位于一个衬底上。 控制对存储单元的访问的集成控制电路位于另一（逻辑电路）基板上。 当读取，写入或擦除存储单元的内容时，控制电路控制序列。 逻辑电路基板还包含CPU和加密协处理器。 存储器电路包括读出放大器，借助于此可以确定存储器单元的存储状态，以及选择字或位线的解码电路。

公开(公告)号：US20100052507A1

公开(公告)日：2010-03-04

申请号：US12312007

申请日：2007-10-22

申请人： Claudia Castiglioni ,  Wolfgang Gruber ,  Armin Konrad

发明人： Claudia Castiglioni ,  Wolfgang Gruber ,  Armin Konrad

IPC分类号： H01J63/04 ,  C09K11/78

CPC分类号： H01J61/44 ,  C09K11/778 ,  C09K11/7787

摘要： The invention relates to a fluorescent coating for Hg low-pressure discharge lamps, comprising a fluorescent composition from at least one green fluorescent material emitting in the green spectral range, especially a Tb- and/or Eu-doped green fluorescent material, and a red fluorescent material emitting in the red spectral range, especially a Eu and/or Mn red fluorescent material. The invention is characterized in that a further fluorescent material is present which is adapted to absorb UV Hg and Hg-Vis radiation.

摘要翻译： 本发明涉及一种用于Hg低压放电灯的荧光涂层，其包括由绿色光谱范围内发射的至少一种绿色荧光材料的荧光组合物，特别是Tb掺杂和/或Eu掺杂的绿色荧光材料，以及红 在红色光谱范围内发射的荧光材料，特别是Eu和/或Mn红色荧光材料。 本发明的特征在于存在适于吸收UV Hg和Hg-Vis辐射的另外的荧光材料。

公开(公告)号：US20060038263A1

公开(公告)日：2006-02-23

申请号：US11213341

申请日：2005-08-25

申请人： Markus Eigner ,  Wolfgang Gruber ,  Manfred Roth ,  Stefan Ruping

发明人： Markus Eigner ,  Wolfgang Gruber ,  Manfred Roth ,  Stefan Ruping

IPC分类号： H01L23/495

CPC分类号： G11C17/10 ,  H01L23/5286 ,  H01L25/0657 ,  H01L2225/06527 ,  H01L2225/06555 ,  H01L2924/0002 ,  H01L2924/00

摘要： A patterned connection plane between two semiconductor chips which are connected using face-to-face technology is patterned into first pads, second pads, and conductor strips which are alternatively connected to one of these pads. The conductor strips are connected to a read-out circuit in one of the semiconductor chips via connections.

摘要翻译： 使用面对面技术连接的两个半导体芯片之间的图案化连接平面被图案化成可替代地连接到这些焊盘之一的第一焊盘，第二焊盘和导体条。 导体条通过连接连接到其中一个半导体芯片中的读出电路。

公开(公告)号：US4049702A

公开(公告)日：1977-09-20

申请号：US589653

申请日：1975-06-23

申请人： Erich Bernt ,  Wolfgang Gruber ,  Erich Haid ,  Fritz Stahler ,  August Wilhelm Wahlefeld ,  Gunter Weimann

发明人： Erich Bernt ,  Wolfgang Gruber ,  Erich Haid ,  Fritz Stahler ,  August Wilhelm Wahlefeld ,  Gunter Weimann

IPC分类号： C12Q1/48 ,  C07C143/67

CPC分类号： C12Q1/48

摘要： .gamma.-Glutamyl-4-nitroanilide compounds of the formula ##STR1## wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyl-transpeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycylglycine and determining the liberation of the resulting p-nitro-aniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

摘要翻译： 其中R是γ-谷氨酰基，X是酸基的γ-谷氨酰基-4-硝基苯胺化合物和这些化合物的盐被用于通过使怀疑有γ-谷氨酰转肽酶的样品接触来测定γ-谷氨酰转肽酶 含有γ-谷氨酰基转肽酶与所述化合物和甘氨酰甘氨酸，并确定所得对硝基苯胺化合物的释放作为样品中最初存在的γ-谷氨酰转肽酶活性的量度。