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公开(公告)号：US5108913A
公开(公告)日：1992-04-28
申请号：US545092
申请日：1990-06-27
申请人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
发明人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
IPC分类号： G01N33/50 , C07H15/04 , C07H15/203 , C12Q1/40
CPC分类号： C12Q1/40 , C12Q2334/10 , Y10S435/805 , Y10S435/81
摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.
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公开(公告)号：US4229527A
公开(公告)日：1980-10-21
申请号：US954139
申请日：1978-10-23
申请人： Joachim Ziegenhorn , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruaber , Hans U. Bergmeyer
发明人： Joachim Ziegenhorn , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruaber , Hans U. Bergmeyer
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/54 , C12Q1/60 , C12Q1/61 , G01N21/75 , G01N33/48 , G01N33/66 , G01N33/92 , C12Q1/50 , C12Q1/44 , C12Q1/48
CPC分类号： C12Q1/61 , C12Q1/00 , C12Q1/54 , C12Q1/60 , Y10S435/81
摘要： Kinetic determination of enzyme substrates by means of coupled reactions comprising selecting the reaction parameters so that the most specific part reaction of a reaction sequence becomes rate-determining for the whole reaction sequence wherein the rate-determining part reaction follows first or pseudo-first order kinetics.
摘要翻译： 通过偶联反应动力学测定酶底物，包括选择反应参数，使得反应序列的最具体的部分反应成为整个反应顺序的速率确定，其中速率确定部分反应遵循第一或伪一级动力学 。
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公开(公告)号：US4184848A
公开(公告)日：1980-01-22
申请号：US836111
申请日：1977-09-23
IPC分类号： C07C69/28 , C12Q1/00 , G01N21/82 , G01N33/48 , G01N33/50 , G01N33/52 , G01N33/16 , G01N31/14
CPC分类号： C12Q1/00 , G01N33/50 , Y10T436/25125
摘要： An agent for the elimination of turbidity in serum, especially in serum samples wherein photometric measurements are to be made, consisting essentially of one or more mono- or di-esters of polyethylene glycol and fatty acids of 9 to 14 carbon atoms with at most 8 ethylene oxide units per fatty acid moiety in the molecule, said esters having an HLB value of from 8 to 12.8.
摘要翻译： 用于消除血清中浊度的试剂，特别是在其中进行光度测量的血清样品中，基本上由一种或多种聚乙二醇和9至14个碳原子的脂肪酸的单酯或二酯组成，最多8个 分子中每个脂肪酸部分的环氧乙烷单元，所述酯的HLB值为8至12.8。
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4.发明授权Process and monoclonal antibody for the specific determination of pancreas alpha-amylase in the presence of saliva alpha-amylase 失效在唾液α-淀粉酶存在下用于特异性测定胰腺α-淀粉酶的方法和单克隆抗体
公开(公告)号：US4939082A
公开(公告)日：1990-07-03
申请号：US326885
申请日：1989-03-21
申请人： Kurt W. Naujoks , Willie Gerhardt , Christa Hubner-Parajsz , Karl Wulff , Herbert Jungfer , Helmut Lenz , Winfried Albert , August W. Wahlefeld
发明人： Kurt W. Naujoks , Willie Gerhardt , Christa Hubner-Parajsz , Karl Wulff , Herbert Jungfer , Helmut Lenz , Winfried Albert , August W. Wahlefeld
IPC分类号： G01N33/537 , C12N5/10 , C12N15/02 , C12P21/08 , C12Q1/40 , C12R1/91 , G01N33/573 , G01N33/577
CPC分类号： C12Q1/40 , G01N33/573 , Y10S435/962 , Y10S436/824 , Y10S436/828 , Y10S530/808 , Y10S530/809
摘要： In body fluids containing saliva alpha amylase and pancreatic alpha amylase, pancreatic alpha amylase is determined with a monoclonal antibody which specifically binds but does not inhibit saliva alpha amylase and which has a cross-reactivity of 5% or less toward pancreatic alpha amylase. The monoclonal antibody binds salvia alpha amylase to form a complex which is separated to permit determining pancreatic alpha amylase with an amylase detection system. The complex may be separated by precipitating with a precipitating agent such as an anti-antibody or protein A, or by immobilizing the monoclonal antibody on a solid carrier. The monoclonal antibody is preferably produced by immunizing a host animal with an immunogen containing aluminum hydroxide, Bordatella pertussis and native saliva alpha amylase at least 7 times over a period of at least 9 months, fusing B lymphocytes of the immunized host animal with a transforming agent to produce hybridomas, cloning and culturing the hybridomas, and screening antibodies formed.
摘要翻译： 在含有唾液α淀粉酶和胰腺α淀粉酶的体液中，用特异性结合但不抑制唾液α-淀粉酶的单克隆抗体测定胰腺α淀粉酶，并且对胰腺α淀粉酶具有5％以下的交叉反应性。 单克隆抗体结合salviaα淀粉酶以形成复合物，其被分离以允许用淀粉酶检测系统测定胰腺α淀粉酶。 可以通过用诸如抗体或蛋白质A的沉淀剂沉淀或通过将单克隆抗体固定在固体载体上来分离复合物。 单克隆抗体优选通过用含有氢氧化铝，百日咳杆菌和天然唾液α-淀粉酶的免疫原在至少9个月的时间内至少7次免疫宿主动物来产生，将免疫宿主动物的B淋巴细胞与转化剂 产生杂交瘤，克隆和培养杂交瘤，并筛选形成的抗体。
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公开(公告)号：US4727025A
公开(公告)日：1988-02-23
申请号：US823489
申请日：1986-01-10
CPC分类号： C12N9/0006 , C12Q1/58 , C12Y101/01154
摘要： For the enzymatic determination of urea, one reacts the urea with glyoxylate in the presence of ureidoglycolate synthetase to give (S)-ureidoglycolate and oxidizes the latter with NAD(P).sup.+ and ureidoglycolate dehydrogenase to carbamoyloxamate and measures NAD(P)H formed either directly or via a color indicator system. A reagent suitable herefor contains glyoxylate, NAD(P).sup.+, (S)-ureidoglycolate synthetase, ureidoglycolate dehydrogenase and buffer substance.
摘要翻译： PCT No.PCT / EP85 / 00250 Sec。 371日期1986年1月10日 102（e）日期1986年1月10日PCT提交1985年5月23日PCT公布。 出版物WO85 / 05641 1985年12月19日。尿素的酶法测定中，尿素与脲醛酸酯在脲基乙酸合成酶的存在下反应，得到（S） - 脲基乙醇酸酯，并用NAD（P）+和脲基乙酸脱氢酶将其氧化成氨基甲酰氨基甲酸酯 NAD（P）H直接或通过颜色指示器系统形成。 适用于此的试剂含有乙醛酸，NAD（P）+，（S） - 脲基乙酸合成酶，脲基乙酸脱氢酶和缓冲物质。
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6.发明授权
公开(公告)号：US4705749A
公开(公告)日：1987-11-10
申请号：US667279
申请日：1984-11-01
CPC分类号： C12Q1/44 , C12Q1/28 , C12Q1/48 , C12Q2326/96 , C12Q2334/00 , G01N2333/904 , G01N2333/9121 , Y10S435/805 , Y10S435/81
摘要： The present invention provides a process for the determination of glycerol in free or bound form by reaction with ATP in the presence of glycerol kinase (GK) and optionally of a hydrolase with the formation of glycerol-3-phosphate and ADP and determination of one of these reaction products with the help of at least one subsequent enzymatic reaction, wherein the determination is carried out kinetically and, for this purpose, the reaction with ATP is made rate-determining for the whole reaction and this is allowed to proceed according to the pseudo-first order in that there is added a sugar of the general formula: ##STR1## in which carbon atoms 2 and 3 have the D-threo configuration and R is a carbohydrate radical containing up to 3 carbon atoms and in which one hydroxyl group can also be replaced by a hydrogen atom.The present invention also provides a reagent for the kinetic determination of glycerol, comprising glycerol kinase, ATP and a system for the determination of glycerol-3-phosphate or of ADP and optionally a hydrolase, wherein it additionally contains a sugar of the general formula: ##STR2## in which carbon atoms 2 and 3 have the D-threo configuration and R is a carbohydrate radical containing up to 3 carbon atoms and in which one hydroxyl group can also be replaced by a hydrogen atom.
摘要翻译： 本发明提供了一种通过在甘油激酶（GK）存在下与ATP反应来测定游离或结合形式的甘油的方法，并且任选地形成甘油-3-磷酸酯和ADP的水解酶，并测定其中之一 这些反应产物借助于至少一个随后的酶反应，其中所述测定在动力学上进行，为此，与ATP的反应对整个反应进行速率确定，并且允许其根据伪 添加了具有以下通式的糖：其中碳原子2和3具有D-苏糖构型并且R是含有至多3个碳原子的碳水化合物基团，其中一个 羟基也可以被氢原子代替。 本发明还提供用于动力学测定甘油的试剂，其包含甘油激酶，ATP和用于测定甘油-3-磷酸或ADP和任选的水解酶的系统，其中其还含有以下通式的糖： 其中碳原子2和3具有D-苏结构，并且R是含有至多3个碳原子的碳水化合物基团，其中一个羟基也可被氢原子代替。
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公开(公告)号：US4337310A
公开(公告)日：1982-06-29
申请号：US184675
申请日：1980-09-08
CPC分类号： C08B31/003 , C12Q1/40
摘要： Method for determining .alpha.-amylase by incubating with a starch substrate, wherein the substrate is based upon particulate starch derivatized with detectable groups for the determination of .alpha.-amylase, the substrate consisting of superficially cross-linked starch or amylose grains having a regulated, reduced swellability, said grains having a diameter of from 0.01 to 0.20 mm; these grains are prepared by suspending non-swollen grains in a solution with only enough cross-linking agent to achieve superficial cross-linking and then derivatizing the grains with the detectable groups for the determination of .alpha.-amylase.
摘要翻译： 通过与淀粉底物孵育来测定α-淀粉酶的方法，其中所述底物是基于用可检测的基团衍生的用于测定α-淀粉酶的颗粒淀粉，所述底物由表面交联的淀粉或直链淀粉颗粒组成，所述淀粉或直链淀粉颗粒具有调节的， 所述颗粒具有0.01至0.20mm的直径; 这些颗粒通过将非溶胀颗粒悬浮在仅具有足够交联剂的溶液中以实现表面交联，然后用可检测的基团衍生化以测定α-淀粉酶来制备。
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公开(公告)号：US4544631A
公开(公告)日：1985-10-01
申请号：US311856
申请日：1981-10-16
申请人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Gerhard Michal , Gunter Weimann
发明人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Gerhard Michal , Gunter Weimann
CPC分类号： C12Q1/40 , C12Q2334/10 , Y10S435/805 , Y10S435/81
摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.
摘要翻译： α-淀粉酶通过α-淀粉酶底物的酶裂解和裂变产物的测定来确定，其中用作底物的麦芽麦角糖化合物，其中R是葡萄糖苷，苯基葡糖苷，单硝基苯基葡糖苷，二硝基苯基葡糖苷， 山梨糖醇或葡萄糖酸基团。 还提供了包含这种底物的试剂和用于通过α-淀粉酶测定由淀粉酶底物形成的裂变产物的系统。
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公开(公告)号：US4162979A
公开(公告)日：1979-07-31
申请号：US843299
申请日：1977-10-18
IPC分类号： B01D15/00 , B01D24/02 , B01D29/085 , B01J8/06 , B01J19/00 , B01J47/02 , B01L3/00 , G01N33/48 , B01D27/02
摘要： A device for the chemical and/or physical treatment of liquids which comprises a flowthrough vessel having an inlet and an outlet and is receptive of a treatment agent therebetween. The vessel comprises a substantially cylindrical tube having one end at the outlet and a conically widening tube section connected to the other end of the cylindrical tube. A permeable carrier is mounted in the cylindrical tube at the outlet end to support the treatment agent placed in the cylindrical tube.
摘要翻译： 一种用于化学和/或物理处理液体的装置，其包括具有入口和出口的流通容器，并且可接受其间的处理剂。 容器包括在出口处具有一端的基本上圆柱形的管和连接到圆柱形管的另一端的锥形加宽管部分。 可渗透的载体在出口端安装在圆柱形管中以支撑放置在圆柱形管中的处理剂。
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