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    Method for measuring cholesterol in low-density lipoprotein, measurement reagent, and measurement kit
    1.
    发明授权
    Method for measuring cholesterol in low-density lipoprotein, measurement reagent, and measurement kit 有权

    公开(公告)号：US12168795B2

    公开(公告)日：2024-12-17

    申请号：US16640231

    申请日：2018-08-30

    Applicant: Hitachi Chemical Diagnostics Systems Co., Ltd.

    Inventor： Mizuki Miura ,  Tomoko Aratake ,  Kenta Kinjo

    IPC: C12Q1/60 ,  C12Q1/32 ,  C12Q1/44

    Abstract: To provide a method for simply and precisely measuring cholesterol (LDL-C) in low-density lipoprotein in a sample without use of any surfactant having an alkylphenol structure, in terms of environmental friendliness. A method for measuring LDL-C in a sample, the method comprising: reacting the sample with (i) a combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) a combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase, in an aqueous solvent which comprises: [a] one or more surfactants selected from the group consisting of a polyoxyethylene alkyl ether and a polyoxyethylene polyoxypropylene alkyl ether; and [b] a polyoxyethylene polyoxypropylene copolymer; and which does not comprise any surfactant having an alkylphenol structure; and measuring a substance formed or consumed in the reaction.

    Method for quantifying subfraction of cholesterol (−C) in high-density lipoprotein (HDL)
    7.
    发明授权
    Method for quantifying subfraction of cholesterol (−C) in high-density lipoprotein (HDL) 有权

    公开(公告)号：US09890412B2

    公开(公告)日：2018-02-13

    申请号：US14391897

    申请日：2013-04-10

    Applicant: Denka Seiken Co., Ltd. ,  National University Corporation Hokkaido University

    Inventor： Yasuki Itoh ,  Hitoshi Chiba

    IPC: C12Q1/60 ,  G01N33/92

    CPC classification number: C12Q1/60 ,  G01N33/92 ,  G01N2333/775

    Abstract: Provided is a method for separately or simultaneously quantifying whole HDL-C and cholesterol in HDL subfractions: ApoE-Containing HDL-C and ApoE-deficient HDL-C. A method for enzymatically and separately quantifying cholesterol in the ApoE-deficient HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative to a test sample in a final concentration of 0.05 to 0.10%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated. A method for enzymatically and separately quantifying cholesterol in ApoE-Containing HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative so as to obtain a final concentration of 0.15 to 0.75%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated.

    BLOOD SAMPLE ASSAY METHOD
    8.
    发明申请
    BLOOD SAMPLE ASSAY METHOD 审中-公开

    公开(公告)号：US20170349933A1

    公开(公告)日：2017-12-07

    申请号：US15629672

    申请日：2017-06-21

    Applicant: AXIS-SHIELD AS

    Inventor： Arne Ludvig FAAREN ,  Frank FRANTZEN ,  Arne Kristian NORDHEI ,  Erling SUNDREHAGEN ,  Lars ØRNING

    IPC: C12Q1/60 ,  G01N33/92 ,  C12Q1/61

    CPC classification number: C12Q1/60 ,  C12Q1/61 ,  G01N33/92

    Abstract: The invention provides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The reagent of step iii) may be applied to the sample separately or may be included in a reagent mixture during steps i) or iv). A corresponding kit is also provided.

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