公开(公告)号：US11719691B2

公开(公告)日：2023-08-08

申请号：US16528440

申请日：2019-07-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Guillermo C. Bazan ,  Brent S. Gaylord ,  Shu Wang ,  Bin Liu

IPC: G01N33/542 ,  C12Q1/6818 ,  G01N33/58 ,  C09B11/02 ,  C09B69/10 ,  C09K9/02 ,  C09K11/06 ,  G01N33/52 ,  B82Y15/00

CPC classification number: G01N33/542 ,  C09B11/02 ,  C09B69/103 ,  C09K9/02 ,  C09K11/06 ,  C12Q1/6818 ,  G01N33/52 ,  G01N33/582 ,  B82Y15/00 ,  C09K2211/1416 ,  C09K2211/1425 ,  C12Q2565/107 ,  C12Q1/6818 ,  C12Q2525/107 ,  C12Q2527/125 ,  C12Q1/6818 ,  C12Q2525/107 ,  C12Q2565/107

Abstract: Light harvesting luminescent multichromophores that are configured upon excitation to transfer energy to, and amplify the emission from, an acceptor signaling chromophore in energy-receiving proximity therewith are provided. Also provided are compositions for labelling a target. The labelling composition may include a donor light harvesting multichromophore and an acceptor signaling chromophore in energy-receiving proximity to the donor light harvesting multichromophore. Also provided is an aqueous composition for labelling a target, including: a donor light harvesting multichromophore; an acceptor signaling chromophore in energy-receiving proximity therewith; and a sensor biomolecule. Methods for using the subject compositions are also provided.

公开(公告)号：US20230194507A1

公开(公告)日：2023-06-22

申请号：US18170481

申请日：2023-02-16

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Spencer Lin ,  Christopher Reamer ,  Ryan Spears

IPC: G01N33/531 ,  G01N33/543 ,  G01N33/52 ,  G01N33/536

CPC classification number: G01N33/531 ,  G01N33/54353 ,  G01N33/52 ,  G01N33/536 ,  G01N33/54306

Abstract: Processes for quantifying an amount of functional groups immobilized on a solid support are described herein. The processes allow for determining whether sufficient functional groups are provided on a solid support for the attachment of a first binding pair member for the detection of a target analyte.

公开(公告)号：US11674953B2

公开(公告)日：2023-06-13

申请号：US16875775

申请日：2020-05-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Benjamin J. Renquist

IPC: G01N33/52 ,  G01N33/50 ,  G01N33/18 ,  A01K67/02 ,  C12Q1/00

CPC classification number: G01N33/52 ,  A01K67/02 ,  C12Q1/008 ,  G01N33/1806 ,  G01N33/5005 ,  G01N33/5088 ,  G01N33/5097 ,  G01N2333/43504 ,  G01N2333/4603

Abstract: Methods for identifying animals that are genetically superior, drugs, nutritional strategies, or physiological manipulations that improve feed efficiency or productivity of animals, e.g., selecting animals that are genetically superior for feed efficiency or productivity based on metabolic rates of particular tissues, wherein metabolic rates of certain tissues such as skeletal muscle are inversely proportional to feed efficiency, while metabolic rates of other tissues such as mammary gland are directly proportional to milk production. Thus, animals with low skeletal muscle metabolic rates are generally more feed efficient, e.g., gain more weight per unit of food. The methods herein may be used to improve the genetics, nutrition, and handling or animals more efficiently produced animal products, e.g., meat production, milk, production, egg production, wool production, etc. The methods herein may also be used to determine estimated breeding values of animals for feed efficiency, growth, or production.

公开(公告)号：US11649311B2

公开(公告)日：2023-05-16

申请号：US16219035

申请日：2018-12-13

Applicant: KIRIN HOLDINGS KABUSHIKI KAISHA ,  THE UNIVERSITY OF TOKYO

Inventor： Toshikazu Tsuji ,  Kumiko Ikado ,  Seiichi Uchiyama ,  Kyoko Kawamoto

IPC: C08F220/60 ,  C08F220/56 ,  C08F228/06 ,  G01N33/52 ,  C08F220/38 ,  C08F28/06

CPC classification number: C08F220/60 ,  C08F220/56 ,  C08F228/06 ,  G01N33/52 ,  C08F28/06 ,  C08F220/38 ,  C08F220/382 ,  C08F220/606

Abstract: There is provided a method for introducing a temperature-sensitive probe comprising a copolymer, which comprises a thermoresponsive unit and a fluorescent unit, into a cell, and the method using the copolymer further comprising a cationic unit as the temperature-sensitive probe, and the method comprising the step of mixing the copolymer with the cell in a solvent. The copolymer can be preferably used as a fluorescence temperature sensor which measures intracellular temperature since the copolymer has a cationic group and thus enters into a cell without using a special method.

公开(公告)号：US20190242879A1

公开(公告)日：2019-08-08

申请号：US16265849

申请日：2019-02-01

Applicant: Simula Innovation

Inventor： Aslak Tveito ,  Samuel Wall ,  Karoline Horgmo Jaeger

IPC: G01N33/50 ,  C12N5/077 ,  G01N33/52 ,  G01N33/68

CPC classification number: G01N33/5061 ,  C12N5/0657 ,  G01N33/5026 ,  G01N33/52 ,  G01N33/6872 ,  G01N2458/30 ,  G01N2500/10 ,  G16B5/20 ,  G16H50/50

Abstract: The present invention provides a method for determining the effect of a drug on a mature cardiomyocyte. In some embodiments, the method comprises using transmembrane voltage and/or intracellular calcium data obtained from control immature cardiomyocytes and those that have been contacted with the drug to parameterize models of immature cardiomyocytes, then applying a maturation matrix to generate a mature cardiomyocyte model. The method is useful for, among other things, predicting whether a drug may have proarrhythmic properties and for determining whether a particular drug should be administered to a patient.

公开(公告)号：US20190241607A1

公开(公告)日：2019-08-08

申请号：US16320570

申请日：2017-07-31

Applicant: NORTH CAROLINA STATE UNIVERSITY

Inventor： David C. Muddiman ,  Philip L. Loziuk ,  Michael Bereman

IPC: C07K1/13 ,  C07B59/00 ,  G01N33/52 ,  C12N9/02 ,  G01N33/573 ,  G01N33/68

CPC classification number: C07K1/13 ,  C07B59/008 ,  C07C229/26 ,  C07K14/00 ,  C12N9/0089 ,  C12Y115/01001 ,  G01N33/52 ,  G01N33/573 ,  G01N33/6848 ,  G01N2800/2835

Abstract: Provided herein are isotopically labeled reagents, including isotopically labeled small molecules and peptides, that can be used to detect and/or quantify β-N-methylamino-L-alanine (BMAA) in the Cu/Zn Superoxide Dismutase 1 (SOD1) protein. Further provided are methods for detecting, preventing, or treating amyotrophic lateral sclerosis in a subject using isotopically labeled reagents to detect and/or quantify β-N-methylamino-L-alanine (BMAA) in the Cu/Zn Superoxide Dismutase 1 (50D1) protein. Further provided are isotopically labeled reagents and methods for detecting BMAA in additional proteins from patient samples.

公开(公告)号：US20180356328A1

公开(公告)日：2018-12-13

申请号：US16003262

申请日：2018-06-08

Applicant: SYSMEX CORPORATION

Inventor： Konobu KIMURA

IPC: G01N15/10 ,  G16H50/30 ,  G01N21/17

CPC classification number: G01N33/569 ,  G01N15/1459 ,  G01N33/52 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1486 ,  G01N2015/1488

Abstract: Disclosed is a particle analysis method for identifying infections, comprising: step A of irradiating light on a measurement sample prepared by mixing a test blood specimen containing particles, collected from a subject, a fluorescent dye that stains a nucleic acid, and a hemolytic agent, thereby acquiring scattered light and fluorescence from each particle contained in the measurement sample, step B of specifying particles that are substantially not contained in blood of control subjects not suffering from infection and are determined to be neutrophils contained in blood of patients with infection, based on the scattered light and the fluorescence, and acquiring particle number information on the specified particles, and step C of determining whether the test blood specimen is a specimen collected from patients with bacterial infection or a specimen collected from patients with viral infection, based on the particle number information.

公开(公告)号：US20180327424A1

公开(公告)日：2018-11-15

申请号：US15595920

申请日：2017-05-15

Applicant: INDICATOR SYSTEMS INTERNATIONAL, INC.

Inventor： Gerald F. SWISS ,  Richard J. PARIZA ,  Robert M. MORIARTY

IPC: C07D519/00 ,  C07H5/06 ,  G01N33/52 ,  G01N33/574

CPC classification number: C07D519/00 ,  C07H5/06 ,  C07H15/26 ,  G01N33/52 ,  G01N33/57496

Abstract: Disclosed are compounds, compositions and methods for detecting remnant cancer cells in a tissue sample.

公开(公告)号：US20180321238A1

公开(公告)日：2018-11-08

申请号：US15770249

申请日：2016-10-24

Applicant: Thomas BRUDERER ,  Madhavi MURANJAN

Inventor： Thomas BRUDERER ,  Madhavi MURANJAN

IPC: G01N33/569 ,  C07K16/12 ,  B01L3/00 ,  G01N33/52

CPC classification number: G01N33/56938 ,  B01L3/5023 ,  C07K16/00 ,  C07K16/1271 ,  G01N33/52

Abstract: A subtractive immunoassay for detecting the presence or absence of first and second analytes in a sample includes in the following order the steps of: a) conjugating a first detector antibody to the first analyte to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte to capture the first analyte to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte to capture the second analyte from the sample; e) detecting presence or absence of the first and second detector tags at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first and second analytes in the sample.

公开(公告)号：US20180321226A1

公开(公告)日：2018-11-08

申请号：US16021941

申请日：2018-06-28

Applicant: Roger GIESE ,  Poguang WANG

Inventor： Roger GIESE ,  Poguang WANG

IPC: G01N33/52 ,  C07C217/48 ,  C07C217/58

CPC classification number: G01N33/52 ,  C07C217/48 ,  C07C217/58 ,  G01N2458/15 ,  G01N2560/00

Abstract: The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.