公开(公告)号：US09091698B2

公开(公告)日：2015-07-28

申请号：US14203367

申请日：2014-03-10

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  Richard L. Moore ,  James Garrett

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/74 ,  G01N33/92

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  G01N33/74 ,  G01N33/92 ,  G01N2333/90209 ,  G01N2333/91051 ,  G01N2800/26

Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

Abstract translation: 本发明涉及可用于例如用于对象中败血症的高级检测的方法，监视器和系统。

公开(公告)号：US11193158B2

公开(公告)日：2021-12-07

申请号：US16734826

申请日：2020-01-06

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483 ,  H01J49/16 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US20180305758A1

公开(公告)日：2018-10-25

申请号：US15889035

申请日：2018-02-05

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  Richard L. Moore ,  James Garrett

IPC: C12Q1/6881 ,  G01N33/74 ,  G01N33/92

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  G01N33/74 ,  G01N33/92 ,  G01N2333/90209 ,  G01N2333/91051 ,  G01N2800/26

Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

公开(公告)号：US20170204448A1

公开(公告)日：2017-07-20

申请号：US15472759

申请日：2017-03-29

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo ,  Mary R. Votta ,  Ben Turng ,  Donald R. Callihan ,  Wendy Louise Williams

IPC: C12Q1/04 ,  G01N33/483

CPC classification number: C12Q1/04 ,  G01N33/4833 ,  G01N33/6851 ,  H01J49/164 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US09671364B2

公开(公告)日：2017-06-06

申请号：US14367549

申请日：2012-12-19

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  James G. Nadeau ,  Michael A. Brasch

IPC: C12Q1/00 ,  C12Q1/04 ,  C12Q1/06 ,  C12N13/00 ,  G01N27/414 ,  B01L3/00 ,  G01N33/487

CPC classification number: G01N27/4145 ,  B01L3/5021 ,  B01L2300/0636 ,  B01L2300/0851 ,  B01L2300/0893 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  C12Q1/04 ,  G01N33/48735 ,  G01N2800/26

Abstract: An array of micro-chambers (220) with individual ion sensitive field effect transistors (ISFETs) (300) disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample (390). In addition to the presence or absence of a single cell, certain further embodiments contemplate monitoring cell behavior. Cell behavior includes the entire range of cell activity as well as cell response to changes in environmental conditions of changes in response due to the addition of sample constituents.