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    METHODS AND COMPOSITIONS FOR MODULATION OF AMPLIFICATION EFFICIENCY
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR MODULATION OF AMPLIFICATION EFFICIENCY 审中-公开
    用于调节放大效率的方法和组合物

    公开(公告)号：US20140193819A1

    公开(公告)日：2014-07-10

    申请号：US14205229

    申请日：2014-03-11

    Applicant: Becton, Dickinson and Company

    Inventor： Tobin Hellyer ,  James G. Nadeau

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2537/143 ,  C12Q2537/161

    Abstract: Provided herein are methods and kits for modulating the amplification efficiency of nucleic acids, which are useful in multiplex reactions where the amplification efficiency of one or more nucleic acids in the mixture are desired to be modulated relative to one or more other nucleic acids. Embodiments relate to molecular diagnostics, including detecting sequence variants, such as SNPs, insertions deletions, and altered methylation patterns, as well as the modulation of the amplification efficiency of internal control sequences to provide more accurate control sequences for amplification reactions.

    Abstract translation: 本文提供用于调节核酸扩增效率的方法和试剂盒，其可用于多反应中，其中期望混合物中一种或多种核酸的扩增效率相对于一种或多种其它核酸进行调节。 实施方案涉及分子诊断，包括检测序列变体，例如SNP，插入缺失和改变的甲基化模式，以及内部控制序列的扩增效率的调节，以提供用于扩增反应的更精确的控制序列。

    SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS
    6.
    发明申请
    SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS 审中-公开
    选择性扩增和实时PCR检测稀有突变体

    公开(公告)号：US20150315636A1

    公开(公告)日：2015-11-05

    申请号：US14439634

    申请日：2013-10-30

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： James G. Nadeau ,  Tobin J. Hellyer

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2535/131 ,  C12Q2537/163

    Abstract: Provided herein are methods and kits for the improved detection of rare mutations within a high background. Exemplary embodiments relate to kits and methods that include amplification primers, a blocking oligonucleotide, and one or more allele-specific detector probes, useful in the specific detection of rare allelic variants or mutations.

    Abstract translation: 本文提供了用于在高背景下改进检测罕见突变的方法和试剂盒。 示例性实施方案涉及包括扩增引物，阻断寡核苷酸和一种或多种等位基因特异性检测器探针的试剂盒和方法，其用于特异性检测罕见的等位基因变体或突变。

    IMMUNO-AMPLIFICATION
    7.
    发明申请
    IMMUNO-AMPLIFICATION 有权
    免疫放大

    公开(公告)号：US20150152473A1

    公开(公告)日：2015-06-04

    申请号：US13739378

    申请日：2013-01-11

    Applicant: Becton, Dickinson and Company

    Inventor： James G. Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha-Sha Wang ,  Keith Edward Thornton

    IPC: C12Q1/68

    CPC classification number: C12Q1/6804 ,  C12Q1/6851

    Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

    Abstract translation: 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

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