公开(公告)号：US20150315636A1

公开(公告)日：2015-11-05

申请号：US14439634

申请日：2013-10-30

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： James G. Nadeau ,  Tobin J. Hellyer

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q2535/131 ,  C12Q2537/163

Abstract: Provided herein are methods and kits for the improved detection of rare mutations within a high background. Exemplary embodiments relate to kits and methods that include amplification primers, a blocking oligonucleotide, and one or more allele-specific detector probes, useful in the specific detection of rare allelic variants or mutations.

Abstract translation: 本文提供了用于在高背景下改进检测罕见突变的方法和试剂盒。 示例性实施方案涉及包括扩增引物，阻断寡核苷酸和一种或多种等位基因特异性检测器探针的试剂盒和方法，其用于特异性检测罕见的等位基因变体或突变。