公开(公告)号：US20150104867A1

公开(公告)日：2015-04-16

申请号：US14268859

申请日：2014-05-02

申请人： Baxter International Inc. ,  Baxter Healthcare SA

发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

IPC分类号： C12N5/071

CPC分类号： C12N5/0043 ,  C12N5/0682 ,  C12N2500/46 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/92

摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。

公开(公告)号：US08846400B2

公开(公告)日：2014-09-30

申请号：US13684680

申请日：2012-11-26

申请人： Technion Research & Development Foundation Ltd.

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/02 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/00

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0603 ,  C12N5/0611 ,  C12N5/0665 ,  C12N5/0695 ,  C12N5/0696 ,  C12N2500/00 ,  C12N2500/99 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/90

摘要： A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith.

摘要翻译： 包含人包皮细胞的细胞培养物，当与其共同培养时，人包皮细胞能够将干细胞保持在未分化状态。

公开(公告)号：US20140273205A1

公开(公告)日：2014-09-18

申请号：US14213094

申请日：2014-03-14

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Paul PRICE ,  Stephen GORFIEN ,  Douglas DANNER ,  Mark PLAVSIC

IPC分类号： C12N5/00

CPC分类号： C12N5/0043 ,  C12N5/06 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/40 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2501/11

摘要： The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.

摘要翻译： 本发明提供能够支持体外培养动物细胞的无血清细胞培养基制剂。 培养基包含至少一种非动物衍生营养素，例如至少一种植物肽和/或至少一种非动物或植物脂质和/或脂肪酸。 培养基还可以任选地包含酵母细胞的酶促消化或提取物。 本发明还提供了使用这些细胞培养基制剂在体外培养动物细胞的方法。 此外，本发明的培养基可以用于病毒生产的动物细胞的生长。

公开(公告)号：US08748174B2

公开(公告)日：2014-06-10

申请号：US13355252

申请日：2012-01-20

申请人： Richard Schwartz ,  John Michael Berry ,  Ajit Subramanian ,  Xiao Shi

发明人： Richard Schwartz ,  John Michael Berry ,  Ajit Subramanian ,  Xiao Shi

IPC分类号： C12N5/00 ,  C12N7/08 ,  C12N5/02

CPC分类号： C12N7/00 ,  C12N5/0037 ,  C12N5/0043 ,  C12N2500/36 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/395 ,  C12N2510/02 ,  C12N2760/16151 ,  C12N2760/16161 ,  C12N2760/16251 ,  C12N2760/16261

摘要： The present invention provides novel MDCK-derived adherent non-tumorigenic cell lines that can be grown in the presence or absence of serum. The cell lines of the present invention are useful for the production of vaccine material (e.g., viruses). More specifically, the cell lines of the present invention are useful for the production of influenza viruses in general and ca/ts influenza viruses in particular. The invention further provides methods and media formulations for the adaptation and cultivation of MDCK cells such that they remain non-tumorigenic. Additionally, the present invention provides methods for the production of vaccine material (e.g., influenza virus) in the novel cell lines of the invention.

摘要翻译： 本发明提供可在血清存在或不存在的情况下生长的新型MDCK衍生的粘附性非致瘤细胞系。 本发明的细胞系可用于生产疫苗材料（例如病毒）。 更具体地说，本发明的细胞系通常用于生产流感病毒，尤其是流感病毒。 本发明还提供了用于适应和培养MDCK细胞的方法和培养基制剂，使得它们保持非致瘤性。 此外，本发明提供了在本发明的新型细胞系中生产疫苗材料（例如流感病毒）的方法。

公开(公告)号：US08722406B2

公开(公告)日：2014-05-13

申请号：US13198476

申请日：2011-08-04

申请人： Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner ,  Leopold Grillberger ,  Artur Mitterer

发明人： Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner ,  Leopold Grillberger ,  Artur Mitterer

IPC分类号： C12N5/07

CPC分类号： C12P21/00 ,  C07K14/755 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0682 ,  C12N2500/05 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/60 ,  C12N2500/76 ,  C12N2500/92

摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

摘要翻译： 描述了用于无蛋白质和无血清培养细胞，特别是哺乳动物细胞的培养基，由此培养基含有一定比例的大豆水解产物。

公开(公告)号：US20140024078A1

公开(公告)日：2014-01-23

申请号：US13945531

申请日：2013-07-18

申请人： GENENTECH, INC.

发明人： Martin Gawlitzek ,  Shun Luo ,  Christina Teresa Petraglia

IPC分类号： C07K16/22 ,  C07K16/18

CPC分类号： C07K16/2878 ,  C07K14/70575 ,  C07K16/18 ,  C07K16/22 ,  C07K2317/14 ,  C07K2319/30 ,  C12N5/0018 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/90

摘要： The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

摘要翻译： 本发明一般涉及补充天冬酰胺的不含谷氨酰胺的细胞培养基。 本发明还涉及在天冬酰胺补充的不含谷氨酰胺的哺乳动物细胞培养物中重组蛋白质如抗体的产生。

公开(公告)号：US20130295667A1

公开(公告)日：2013-11-07

申请号：US13847961

申请日：2013-03-20

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Stephen GORFIEN ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David Epstein ,  Dale Gruber ,  Paul Price

IPC分类号： C12N5/071 ,  C12N5/073

CPC分类号： C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

公开(公告)号：US20130252329A1

公开(公告)日：2013-09-26

申请号：US13909128

申请日：2013-06-04

申请人： Technion Research & Development Foundation Limited

发明人： Michal AMIT ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/0735

CPC分类号： C12N5/0606 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0602 ,  C12N5/0603 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2501/2306 ,  C12N2506/02 ,  C12N2533/52

摘要： Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of mainataining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.

摘要翻译： 包含TGF-β同种型或在IL6和可溶性IL6受体（IL6RIL6）之间形成的嵌合体的定义良好的，不含异种的培养基，其能够将干细胞，特别是人类胚胎干细胞在未分化的 状态。 还提供了包含培养基和干细胞的细胞培养物以及在这种定义良好的不含异种植物的培养基中扩增和衍生胚胎干细胞的方法。 此外，本发明提供了区分由其形成的ESC或用于产生谱系特异性细胞的EBs的方法。

公开(公告)号：US08329465B2

公开(公告)日：2012-12-11

申请号：US12986111

申请日：2011-01-06

申请人： Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

发明人： Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

IPC分类号： C12N15/85 ,  C12N15/09 ,  C12P21/00

CPC分类号： C12N5/0075 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/50 ,  C12N2500/76 ,  C12N2531/00

摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.

摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质，以及制备重组蛋白质的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。

公开(公告)号：US08318489B2

公开(公告)日：2012-11-27

申请号：US12158521

申请日：2006-12-22

申请人： Bruce Paul Davidson ,  Ralph Eberhard Graichen ,  Robert Zweigerdt ,  Xiuqin Xu ,  Christine Lindsay Mummery ,  William Sun

发明人： Bruce Paul Davidson ,  Ralph Eberhard Graichen ,  Robert Zweigerdt ,  Xiuqin Xu ,  Christine Lindsay Mummery ,  William Sun

IPC分类号： C12N5/02

CPC分类号： C12N5/0657 ,  A61K35/12 ,  C12N5/0043 ,  C12N2500/25 ,  C12N2500/99 ,  C12N2501/02 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2502/30 ,  C12N2506/02 ,  C12N2510/00 ,  G01N33/502 ,  G01N33/5061

摘要： The present invention relates to the induction of differentiation in stem cells to cardiomyocytes and factors such as prostaglandin alone or in combination with other factors including essential minerals selected from the group including transferrin and selenium, small molecules selected from the group including a p38 MAPK inhibitor such as SB203580 and protein growth factors of the FGF, IGF and BMP families such as but not limited to IGF1, FGF2, BMP2, BMP4 and BMP6. and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from stem cells is also provided wherein the media contains these factors. The use of cardiomyocytes and cardiac progenitors produced by the directed differentiation in transplantation and screening for cardiac compounds is also provided.

摘要翻译： 本发明涉及诱导干细胞分化为心肌细胞和诸如前列腺素的因素，或与其它因素组合，包括选自包括转铁蛋白和硒的必需矿物质，选自包括p38MAPK抑制剂在内的小分子 作为SB203580和FGF，IGF和BMP家族的蛋白生长因子，例如但不限于IGF1，FGF2，BMP2，BMP4和BMP6。 和影响心肌细胞分化过程的胰岛素。 还提供了适合于诱导心肌细胞从干细胞分化的培养基，其中培养基含有这些因子。 还提供了通过移植中的定向分化和心脏化合物筛选产生的心肌细胞和心脏祖细胞的使用。