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公开(公告)号：US20130295667A1
公开(公告)日：2013-11-07
申请号：US13847961
申请日：2013-03-20
发明人： Stephen GORFIEN , Richard Fike , Glenn Godwin , Joyce Dzimian , David Epstein , Dale Gruber , Paul Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
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公开(公告)号：US09879243B2
公开(公告)日：2018-01-30
申请号：US13968157
申请日：2013-08-15
CPC分类号： C12N9/2471 , C12N5/0018 , C12N5/0043 , C12N5/005 , C12N15/88 , C12N2500/22 , C12N2500/24 , C12N2501/999 , C12N2510/02
摘要： The present invention is directed generally to cell culture media useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells) in the presence of said media. Cells containing introduced materials can be further cultured in the media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention thus provides efficient and high throughput methods to transform/transfect culture and cells avoiding the need for multiple manipulations and transfers of cells during transfection and expression studies.
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公开(公告)号：US08815573B2
公开(公告)日：2014-08-26
申请号：US13847961
申请日：2013-03-20
发明人： Stephen F. Gorfien , Richard Fike , Glenn P. Godwin , Joyce L. Wolanske , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells. The invention also provides kits for use in the cultivation of a mammalian epithelial cell that comprise one or more containers, wherein a first container contains the culture medium of the invention. These kits may further comprise one or more additional containers containing one or more supplements.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。 本发明还提供用于培养包含一个或多个容器的哺乳动物上皮细胞的试剂盒，其中第一容器含有本发明的培养基。 这些试剂盒还可以包含一种或多种含有一种或多种补充剂的另外的容器。
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公开(公告)号：US20140057335A1
公开(公告)日：2014-02-27
申请号：US13968157
申请日：2013-08-15
CPC分类号： C12N9/2471 , C12N5/0018 , C12N5/0043 , C12N5/005 , C12N15/88 , C12N2500/22 , C12N2500/24 , C12N2501/999 , C12N2510/02
摘要： The present invention is directed generally to cell culture media useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells in the presence of said media. Cells containing introduced materials can be further cultured in the media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention thus provides efficient and high throughput methods to transform/transfect culture and cells avoiding the need for multiple manipulations and transfers of cells during transfection and expression studies.
摘要翻译： 本发明一般涉及用于将大分子和化合物（例如，核酸分子）引入细胞的细胞培养基（例如，在所述培养基存在下的真核细胞），含有引入的物质的细胞可以在培养基中进一步培养 特别地，本发明允许将核酸分子（例如，载体）引入细胞（特别是真核细胞）中，以及由细胞中的核酸分子编码的蛋白质的表达。本发明无需每次更换细胞培养基 因此，本发明提供了有效和高通量的方法来转化/转染培养物和细胞，避免了在转染和表达研究期间对细胞进行多重操作和转移的需要。
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