公开(公告)号：US20140273205A1

公开(公告)日：2014-09-18

申请号：US14213094

申请日：2014-03-14

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Paul PRICE ,  Stephen GORFIEN ,  Douglas DANNER ,  Mark PLAVSIC

IPC分类号： C12N5/00

CPC分类号： C12N5/0043 ,  C12N5/06 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/40 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2501/11

摘要： The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.

摘要翻译： 本发明提供能够支持体外培养动物细胞的无血清细胞培养基制剂。 培养基包含至少一种非动物衍生营养素，例如至少一种植物肽和/或至少一种非动物或植物脂质和/或脂肪酸。 培养基还可以任选地包含酵母细胞的酶促消化或提取物。 本发明还提供了使用这些细胞培养基制剂在体外培养动物细胞的方法。 此外，本发明的培养基可以用于病毒生产的动物细胞的生长。