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公开(公告)号：US09458222B2
公开(公告)日：2016-10-04
申请号：US13179399
申请日：2011-07-08
IPC分类号： C07K14/755 , C12N9/64 , A61K38/00
CPC分类号： C07K14/755 , A61K9/0019 , A61K38/00 , A61K38/36 , A61K38/37 , C12N9/6489 , C12N2500/24 , C12N2511/00 , C12P21/00 , C12Y304/24087
摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
摘要翻译： 在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS13的细胞培养条件。 本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养物上清液。 本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。
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2.发明授权Method of isolation and purification of trypsin from pronase protease and use thereof 有权从链霉蛋白酶分离和纯化胰蛋白酶的方法及其用途公开(公告)号：US08389261B2
公开(公告)日：2013-03-05
申请号：US12648170
申请日：2009-12-28
申请人： Artur Mitterer , Christa Tauer , Manfred Reiter , Wolfgang Mundt
发明人： Artur Mitterer , Christa Tauer , Manfred Reiter , Wolfgang Mundt
CPC分类号： C12N9/6427 , C07K14/36 , C12N7/00 , C12N2760/16051 , C12N2760/16151 , C12N2770/32451
摘要： The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
摘要翻译： 本发明提供了在单亲和色谱步骤中从PRONASE蛋白酶混合物分离和纯化灰色链霉菌胰蛋白酶（SGT）的方法和纯化的SGT的用途。
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3.发明申请METHOD OF ISOLATION AND PURIFICATION OF TRYPSIN FROM PRONASE PROTEASE AND USE THEREOF 有权从抗原蛋白分离和纯化TRYPSIN的方法及其用途公开(公告)号：US20120003691A1
公开(公告)日：2012-01-05
申请号：US12648170
申请日：2009-12-28
申请人： Artur Mitterer , Christa Tauer , Manfred Reiter , Wolfgang Mundt
发明人： Artur Mitterer , Christa Tauer , Manfred Reiter , Wolfgang Mundt
IPC分类号： C12P21/06 , C12N5/071 , C12N5/10 , C07K1/14 , C12P21/00 , C07K14/00 , C07K14/10 , C07K14/11 , C12N7/00 , C12N7/02
CPC分类号： C12N9/6427 , C07K14/36 , C12N7/00 , C12N2760/16051 , C12N2760/16151 , C12N2770/32451
摘要： The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
摘要翻译： 本发明提供了在单亲和色谱步骤中从PRONASE蛋白酶混合物分离和纯化灰色链霉菌胰蛋白酶（SGT）的方法和纯化的SGT的用途。
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4.发明授权公开(公告)号：US08080414B2
公开(公告)日：2011-12-20
申请号：US12488465
申请日：2009-06-19
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
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公开(公告)号：US20110287482A1
公开(公告)日：2011-11-24
申请号：US13198476
申请日：2011-08-04
CPC分类号： C12P21/00 , C07K14/755 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0682 , C12N2500/05 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/60 , C12N2500/76 , C12N2500/92
摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
摘要翻译： 描述了用于无蛋白质和无血清培养细胞，特别是哺乳动物细胞的培养基，由此培养基含有一定比例的大豆水解产物。
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公开(公告)号：US08021881B2
公开(公告)日：2011-09-20
申请号：US11841915
申请日：2007-08-20
IPC分类号： C12N5/07
CPC分类号： C12P21/00 , C07K14/755 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0682 , C12N2500/05 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/60 , C12N2500/76 , C12N2500/92
摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
摘要翻译： 描述了用于无蛋白质和无血清培养细胞，特别是哺乳动物细胞的培养基，由此培养基含有一定比例的大豆水解产物。
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公开(公告)号：US20110151512A1
公开(公告)日：2011-06-23
申请号：US13035696
申请日：2011-02-25
CPC分类号： C07K14/755 , C12N5/0018 , C12N5/005 , C12N7/00 , C12N2500/05 , C12N2500/10 , C12N2500/20 , C12N2500/24 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2710/24151
摘要： The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.
摘要翻译： 本发明涉及包含至少0.5mg / L多胺的不含寡肽的细胞培养基和用于在所述不含寡肽的细胞培养基中培养包含至少0.5mg / L多胺的细胞的方法。 本发明还涉及用于在包含至少0.5mg / L多胺的培养基中表达至少一种蛋白质的方法和用于在包含至少0.5mg / L多胺的培养基中产生至少一种病毒的方法。
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公开(公告)号：US20110081722A1
公开(公告)日：2011-04-07
申请号：US12781661
申请日：2010-05-17
IPC分类号： C12N5/071
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。
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公开(公告)号：US20100120093A1
公开(公告)日：2010-05-13
申请号：US12612752
申请日：2009-11-05
申请人： Simone von Fircks , Ruth Elmer , Manfred Reiter
发明人： Simone von Fircks , Ruth Elmer , Manfred Reiter
CPC分类号： C12N9/647 , C12N9/6437 , C12N2500/95 , C12Y304/21021
摘要： The invention provides methods and compositions for the serum-free, insulin-free production of recombinant Factor VII.
摘要翻译： 本发明提供了重组因子VII的无血清，无胰岛素生产的方法和组合物。
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10.发明申请RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING THE SAME 有权具有增加的稳定性的重组细胞克隆及其制备和使用方法公开(公告)号：US20090253178A1
公开(公告)日：2009-10-08
申请号：US12488465
申请日：2009-06-19
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质，以及制备重组蛋白质的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
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