公开(公告)号：US20240175010A1

公开(公告)日：2024-05-30

申请号：US18476486

申请日：2023-09-28

Applicant: Illumina, Inc.

Inventor： Allison Yunghans ,  Angelica Marie Barr Schalembier ,  Kayla Busby ,  Stephen M. Gross

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/22 ,  C12N9/24 ,  C12Q1/6874

CPC classification number: C12N15/1082 ,  C12N9/22 ,  C12N9/2497 ,  C12N9/93 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Y302/0202 ,  C12Y302/02023 ,  C12Y302/02027 ,  C12Y302/02029 ,  C12Y605/01001

Abstract: Disclosed herein is a modified transposon end sequence comprising a mosaic end sequence, wherein the mosaic end sequence comprises one or more mutation as compared to a wild-type mosaic end sequence, wherein the mutation comprises a substitution with a uracil, an inosine, a ribose, an 8-oxoguanine, a thymine glycol, a modified purine, or a modified pyrimidine. Also disclosed are transposome complexes comprising these modified transposon end sequences and methods of library preparation using these modified transposon end sequences.

公开(公告)号：US20240132958A1

公开(公告)日：2024-04-25

申请号：US18403902

申请日：2024-01-04

Applicant: Fluent Biosciences Inc.

Inventor： Sepehr Kiani

IPC: C12Q1/6876 ,  C12N15/10 ,  C12Q1/686

CPC classification number: C12Q1/6876 ,  C12N15/1096 ,  C12Q1/686

Abstract: This disclosure provides methods and systems for single-cell, multi-omic analysis of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling.

公开(公告)号：US11932849B2

公开(公告)日：2024-03-19

申请号：US16677012

申请日：2019-11-07

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12N15/10 ,  C40B40/06 ,  C40B50/04 ,  C40B50/06 ,  C40B70/00

CPC classification number: C12N15/1096 ,  C12N15/1006 ,  C40B40/06 ,  C40B50/04 ,  C40B50/06 ,  C40B70/00

Abstract: Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.

公开(公告)号：US20240067953A1

公开(公告)日：2024-02-29

申请号：US18301565

申请日：2023-04-17

Applicant: 10x Genomics, Inc.

Inventor： Tarjei Sigurd MIKKELSEN ,  Phillip BELGRADER ,  Xinying ZHENG

IPC: C12N15/10 ,  C12N5/071 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12N15/1003 ,  C12N5/0602 ,  C12N15/1062 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/1096 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6855

Abstract: The present disclosure provides methods, compositions and systems for analyzing individual cells or cell populations through a partitioned analysis of contents of individual cells or cell populations, such as cancer cells and cells of the immune system. Individual cells or cell populations may be co-partitioned with processing reagents for accessing cellular contents, and for uniquely identifying the content of a given cell or cell population, and subsequently analyzing the content of the cell and characterizing it as having derived from an individual cell or cell population, including analysis and characterization of nucleic acid(s) from the cell through sequencing.

公开(公告)号：US20230392140A1

公开(公告)日：2023-12-07

申请号：US18304993

申请日：2023-04-21

Applicant: Synthorx, Inc. ,  The Scripps Research Institute

Inventor： Floyd E. ROMESBERG ,  Xiyu Dong ,  Anne Xiaozhou Zhou

IPC: C12N15/10 ,  C12N9/12

CPC classification number: C12N15/1048 ,  C12N9/1276 ,  C12Y207/07049 ,  C12N15/1096

Abstract: Disclosed herein are methods of reverse transcribing a polynucleotide comprising an unnatural ribonucleotide comprising reverse transcribing the polynucleotide with a reverse transcriptase in the presence of an unnatural dNTP comprising an unnatural nucleobase, wherein the reverse transcriptase polymerizes cDNA into which the unnatural NTP is incorporated. In some embodiments, the polynucleotide is present at a concentration less than or equal to about 500 nM and/or the polynucleotide is a tRNA, mRNA, RNA aptamer, or a member of a plurality of RNA aptamer candidates.

公开(公告)号：US11795515B2

公开(公告)日：2023-10-24

申请号：US17210637

申请日：2021-03-24

Applicant: INSTITUT PASTER

Inventor： Flora Donati ,  Mélanie Albert ,  Sylvie Behillil ,  Vincent Enouf ,  Sylvie van der Werf

IPC: C12Q1/68 ,  C12Q1/70 ,  C12N15/10

CPC classification number: C12Q1/701 ,  C12N15/1096

Abstract: Methods, primers, sets of primers, probes, compositions, and kits for detecting presence or absence of SARS-CoV-2 in a sample are provided.

公开(公告)号：US11781178B2

公开(公告)日：2023-10-10

申请号：US16772682

申请日：2018-12-17

Applicant: BAYLOR COLLEGE OF MEDICINE

Inventor： William K. Decker ,  Vanaja Konduri

IPC: C12Q1/6853 ,  C12N15/10

CPC classification number: C12Q1/6853 ,  C12N15/1096 ,  C12Q2521/107

Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.

公开(公告)号：US20230313289A1

公开(公告)日：2023-10-05

申请号：US18076214

申请日：2022-12-06

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

IPC: C12Q1/6869 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6869 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/178 ,  C12Y207/07049

Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

公开(公告)号：US11767557B2

公开(公告)日：2023-09-26

申请号：US16213551

申请日：2018-12-07

Applicant: Massachusetts Institute of Technology

Inventor： J. Christopher Love ,  Todd Michael Gierahn ,  Alexander K. Shalek ,  Marc Havens Wadsworth ,  Travis K. Hughes

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12N15/10 ,  G03F7/20 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  C12Q1/6806 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12N15/1093 ,  C12Q2533/101 ,  C12Q2563/179 ,  C12N15/1096 ,  C12Q2525/173 ,  C12Q2533/101 ,  C12Q2563/179

Abstract: Modifications to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays are disclosed.

公开(公告)号：US20230250482A1

公开(公告)日：2023-08-10

申请号：US17825669

申请日：2022-05-26

Applicant: LUCENCE LIFE SCIENCES PTE. LTD.

Inventor： Yukti CHOUDHURY ,  Chae Yin CHER ,  Jia Min HO ,  Min-Han TAN ,  Kao Chin NGEOW ,  Pannapa PINWEHA

IPC: C12Q1/6886 ,  C12N15/10

CPC classification number: C12Q1/6886 ,  C12N15/1065 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q2600/16 ,  C12Q2600/158

Abstract: Disclosed is a method of detecting and quantifying genomic and gene expression alterations using RNA in a biological sample. The disclosed method may include determining presence or absence of the genomic alteration and/or determining presence or absence of the gene expression and/or quantifying the level of the gene expression, by performing variant calling of the sequence alignment obtained from the disclosed method. Variant calling may comprise the steps of identifying differences between a consensus read and a reference genome based on the sequence alignment from the disclosed method; and determining the read count of sequence alignments comprising genomic alteration. The genomic alteration may be an insertion (such as a duplication), a deletion, a single nucleotide variant, or combinations thereof. Also disclosed is a kit for detecting and quantifying genomic and gene expression alterations using RNA in a biological sample.