公开(公告)号：US11921122B2

公开(公告)日：2024-03-05

申请号：US18107947

申请日：2023-02-09

申请人： Quest Diagnostics Investments Incorporated

发明人： Nigel J. Clarke ,  Brett Holmquist ,  Gloria Kwang-Ja Lee ,  Richard E. Reitz

IPC分类号： G01N33/82 ,  G01N30/72 ,  G01N30/88 ,  H01J49/00

CPC分类号： G01N33/82 ,  G01N30/7233 ,  G01N30/72 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/00 ,  H01J49/004 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

摘要： Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

公开(公告)号：US11719690B2

公开(公告)日：2023-08-08

申请号：US17030167

申请日：2020-09-23

申请人： Plasma Technologies, LLC

发明人： Eugene Zurlo ,  Dennis Curtin

IPC分类号： B01D15/12 ,  B01D15/38 ,  C07K1/18 ,  C07K1/22 ,  C07K1/32 ,  C07K14/81 ,  G01N30/02 ,  G01N33/49 ,  G01N30/88

CPC分类号： G01N33/491 ,  B01D15/12 ,  B01D15/3804 ,  C07K1/18 ,  C07K1/22 ,  C07K1/32 ,  C07K14/81 ,  C07K14/8125 ,  G01N30/02 ,  G01N2030/8822

摘要： A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields.

公开(公告)号：US20190234917A1

公开(公告)日：2019-08-01

申请号：US16265571

申请日：2019-02-01

申请人： The Cleveland Clinic Foundation

发明人： Stanley L. Hazen ,  Zeneng Wang ,  Bruce L. Levison

IPC分类号： G01N30/88 ,  A61K31/7036 ,  C12Q1/02 ,  A61K31/43 ,  A61K31/4164 ,  A61K31/496 ,  C12Q1/18 ,  G01N33/68

CPC分类号： G01N30/88 ,  A61K31/4164 ,  A61K31/43 ,  A61K31/496 ,  A61K31/7036 ,  C12Q1/025 ,  C12Q1/18 ,  G01N30/7233 ,  G01N33/6893 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2800/04 ,  G01N2800/042 ,  G01N2800/044 ,  G01N2800/32 ,  G01N2800/323 ,  G01N2800/50 ,  G01N2800/52 ,  G01N2030/884

摘要： The present invention provides markers and methods for determining whether a subject, particularly a human subject, has or is at risk of developing, a disease such as cardiovascular disease, diabetes mellitus, insulin resistance, metabolic syndrome, NAFLD (Nonalcoholic Fatty Liver Disease) or NASH (Nonalcoholic Steatohepatitis) (e.g., within the ensuing year, two years, and/or three years). The present application also relates to the use of such markers and methods for monitoring the status of such diseases in a subject or the effects of therapeutic agents on subjects with such diseases.

公开(公告)号：US20180088132A1

公开(公告)日：2018-03-29

申请号：US15599419

申请日：2017-05-18

申请人： Lianwen QI

发明人： Lianwen QI ,  Yong FAN ,  Ping LI ,  Wei ZHU ,  Yan CHEN

IPC分类号： G01N33/68 ,  G01N30/96 ,  G06F19/00

CPC分类号： G01N33/6893 ,  G01N30/7206 ,  G01N30/7233 ,  G01N33/492 ,  G01N33/6815 ,  G01N33/88 ,  G01N33/92 ,  G01N2030/8822 ,  G01N2560/00 ,  G01N2570/00 ,  G01N2800/324 ,  G16H50/20

摘要： This application discloses a method of differentiating stable angina pectoris from acute coronary syndrome, including: obtaining a blood plasma sample from a patient; measuring a relative concentration of at least one metabolic biomarker in the blood plasma sample, wherein the at least one metabolic biomarker is selected from the group consisting of malic acid, taurine, arachidonic acid, citramalic acid, methionine, and pentadecanoic acid; calculating a value according to the relative concentration of at least one metabolic biomarker; comparing the value with a predefined critical value, if the value is more than the predefined critical value, the patient has the acute coronary syndrome, otherwise, the patient has the stable angina pectoris. The method and a diagnostic kit thereof is capable of differential diagnosis of SA and ACS and it can improve the diagnostic convenience and promote the diagnostic standardization.

公开(公告)号：US09889447B2

公开(公告)日：2018-02-13

申请号：US14908073

申请日：2014-07-25

申请人： SEKISUI MEDICAL CO., LTD. ,  SEKISUI TECHNO MOLDING CO., LTD.

发明人： Yuriko Nemoto ,  Hiroaki Taira ,  Takuya Yotani ,  Satoru Tominaga

IPC分类号： A61J1/06 ,  B01L3/00 ,  G01N21/00 ,  G01N1/10 ,  G01N35/10 ,  G01N30/02 ,  G01N30/88

CPC分类号： B01L3/52 ,  B01L3/523 ,  B01L3/527 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0672 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/123 ,  G01N30/02 ,  G01N35/1002 ,  G01N35/1079 ,  G01N2030/027 ,  G01N2030/8822

摘要： Provided are a reagent supply method and device capable of using up a reagent in a container without a remnant to the extent possible, and supplying the reagent to an analysis device without mixing air bubbles even when the remaining amount of the reagent is extremely small. The reagent supply device includes: at least one reagent container including: a container body for storing a liquid reagent; and a reagent supply port arranged on the container body; and a support member for supporting the at least one reagent container so that the reagent supply port is positioned on a lower side of the container body. The reagent supply port is sealed by a plug including at least one nozzle penetration portion. The at least one nozzle penetration portion is formed of a material having a Shore hardness of from A5° to A90°.

公开(公告)号：US09810604B2

公开(公告)日：2017-11-07

申请号：US14781200

申请日：2014-03-27

申请人： Sekisui Medical Co., Ltd.

发明人： Takuya Yotani ,  Takayuki Oka ,  Hideki Muraki

IPC分类号： G01N35/10 ,  G01N1/14 ,  G01N30/16 ,  G01N15/14 ,  G01N33/49 ,  G01N30/18 ,  G01N30/88

CPC分类号： G01N1/14 ,  G01N15/1429 ,  G01N30/16 ,  G01N30/18 ,  G01N33/491 ,  G01N33/4915 ,  G01N35/1016 ,  G01N35/1095 ,  G01N2015/1486 ,  G01N2030/8822

摘要： Provided is a sample injection device for flow-type analysis including a cylindrical needle (27) which penetrates through an upper wall and a lower wall of a sample injection portion (22) of a carrier-liquid channel through ring-like sealing members (25, 26). The needle (27) includes an inner hole (41) which is closed on a side of a lower end of the needle (27) and open on an outer peripheral surface as a horizontal hole (42). The needle moving unit (44) induces the needle (27) to move downward so that the horizontal hole (42) faces an inside of a sample vessel (40) to draw the sample to the inside of the needle (27). Then the moving unit (44) induces the needle (27) to move upward so that the horizontal hole (42) faces an inside of the sample injection portion (22) to inject the sample in the inside of the needle (27). At an intermediate position, washing liquid is discharged from the horizontal hole (42) of the needle (27), and the washing liquid is recovered via a discharge path (15).

公开(公告)号：US09643104B2

公开(公告)日：2017-05-09

申请号：US14453400

申请日：2014-08-06

申请人： ARKRAY, Inc.

发明人： Takeshi Matsubara

IPC分类号： B01D15/42 ,  B01D15/26 ,  G01N30/24 ,  G01N30/88 ,  B01D15/16 ,  G01N30/34 ,  G01N30/02 ,  B01D19/00 ,  G01N30/20

CPC分类号： B01D15/424 ,  B01D15/166 ,  B01D15/265 ,  B01D19/00 ,  G01N30/24 ,  G01N30/34 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/201 ,  G01N2030/202 ,  G01N2030/207 ,  G01N2030/8804 ,  G01N2030/8822

摘要： A liquid chromatography device includes an adsorption portion that adsorbs one or more analysis components in a specimen, an analysis device that analyzes an analysis component eluted by an eluent, a main feeding device that feeds a first eluent to the adsorption portion, the first eluent eluting an analysis component, a feeding channel in fluid communication with the main feeding device and the adsorption portion, a first retention channel that retains a second eluent, the second eluent differing from the first eluent, an auxiliary feeding device that feeds the second eluent to the first retention channel, and a first switching device that switches the feeding channel to either a first channel or a second channel, the first channel allowing the first eluent to flow from the main feeding device to the adsorption portion, and the second channel allowing the second eluent to flow from the first retention channel to the adsorption portion.

公开(公告)号：US09429579B2

公开(公告)日：2016-08-30

申请号：US12311175

申请日：2007-09-20

申请人： Koji Sugiyama ,  Tatsuo Kamata ,  Takeshi Takagi

发明人： Koji Sugiyama ,  Tatsuo Kamata ,  Takeshi Takagi

IPC分类号： G01N33/00 ,  G01N35/00 ,  G01N33/66 ,  G01N33/72 ,  G01N35/02 ,  A61B5/145 ,  G01N30/88

CPC分类号： G01N33/66 ,  A61B5/14532 ,  G01N33/723 ,  G01N35/026 ,  G01N2030/8822 ,  G01N2800/042

摘要： The present invention relates to a blood analysis apparatus X for measuring concentrations of glucose and glycohemoglobin in blood. The blood analysis apparatus X is configured to perform the concentration measurement of the glucose and the glycohemoglobin by one sampling of blood 13. The blood analysis apparatus X is preferably configured to simultaneously carry out sample preparations for concentration measurement of the glucose and the glycohemoglobin by one sample preparation. The blood analysis apparatus X may be configured to perform dilution of a blood sample for measuring the glycohemoglobin and dilution of a blood sample for measuring the glucose using the same diluent.

摘要翻译： 血液分析装置技术领域本发明涉及用于测定血液中葡萄糖和糖血红蛋白浓度的血液分析装置X. 血液分析装置X构成为通过血液13的一次取样来进行葡萄糖和糖血红蛋白的浓度测定。血液分析装置X优选同时进行葡萄糖和糖血红蛋白的浓度测定用样品制剂1 样品制备。 血液分析装置X可以被配置为执行用于测量糖血红蛋白的血液样品的稀释和用于使用相同的稀释剂测量葡萄糖的血液样品的稀释。

公开(公告)号：US20160153942A1

公开(公告)日：2016-06-02

申请号：US14905472

申请日：2014-07-17

申请人： SEKISUI MEDICAL CO., LTD.

发明人： Takuya Yotani ,  Hiroaki Taira ,  Takayuki Oka ,  Hideki Muraki

IPC分类号： G01N30/34 ,  G01N33/49 ,  G01N30/16

CPC分类号： G01N30/34 ,  G01N30/16 ,  G01N30/88 ,  G01N33/491 ,  G01N2030/027 ,  G01N2030/347 ,  G01N2030/8822

摘要： A gradient liquid feed device allows reduction in size of a high performance liquid chromatography type sample analyzer. The gradient liquid feed device includes a plurality of carrier liquid reservoir tanks configured to store carrier liquids of mutually different compositions, a plurality of single plunger pumps capable of drawing and discharging the carrier liquid from the plurality of carrier liquid reservoir tanks, a mixer configured to mix the carrier liquids discharged from the plurality of liquid feed pumps and feed the mixed carrier liquid, and a pulse damper in communication with the mixer and configured to absorb pulsation that may occur during liquid feeding. The single plunger pumps have a function for variably setting a length of stroke for drawing and discharging the carrier liquid and a function for variably setting a ratio between the carrier liquid drawing time and the carrier liquid discharge time.

摘要翻译： 梯度液体供给装置允许降低高效液相色谱型样品分析仪的尺寸。 梯度液体供给装置包括：多个载液储存箱，其配置成储存相互不同组成的载液;多个单柱塞泵，能够从多个载液储罐中抽吸和排出载液;混合器， 混合从多个液体供给泵排出的载液，并输送混合载液，以及与混合器连通的脉冲阻尼器，并配置成吸收在液体供给期间可能发生的脉动。 单柱塞泵具有用于可变地设定用于抽吸和排出载液的行程长度的功能，以及用于可变地设定载液时间与载液排出时间之间的比率的功能。

公开(公告)号：US20160103103A1

公开(公告)日：2016-04-14

申请号：US14775402

申请日：2014-03-11

申请人： SEKISUI MEDICAL CO., LTD.

发明人： Takuya Yotani ,  Takayuki Oka ,  Hideki Muraki

IPC分类号： G01N30/60 ,  G01N30/16

CPC分类号： G01N30/6026 ,  G01N30/16 ,  G01N30/6004 ,  G01N30/603 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/606 ,  G01N2030/8822

摘要： A cylindrical column body (101) holds a filler. A pair of end caps (105, 106) covers both ends of the column body (101) and has a flow hole for a carrier liquid (111, 112) arranged in the center thereof. An end surface on the side of a large diameter portion (113a, 114a) of a pair of columnar joint members (113, 114) contacts an end surface of the pair of end caps (105, 106) and also has a communication hole (115, 116) arranged in the center thereof. A sealing member (117, 118) is arranged on a contact surface between the end cap (105, 106) and the joint member (113, 114). A bottomed cylindrical case (121) accommodates the pair of end caps (105, 106) and a large diameter portion of the pair of joint members (113, 114) in an engaged state. A cover member (124) is detachably installed on a side of an opening of the case (121).

摘要翻译： 圆柱形柱体（101）保持填料。 一对端盖（105,106）覆盖柱体（101）的两端，并具有用于布置在其中心的载液（111,112）的流动孔。 一对柱状接头构件（113,114）的大径部（113a，114a）侧的端面与一对端盖（105,106）的端面接触，并且还具有连通孔 115,116）。 密封构件（117,118）布置在端盖（105,106）和接头构件（113,114）之间的接触表面上。 有底的圆筒形壳体（121）在一对接合状态下容纳一对端盖（105,106）和一对接头构件（113,114）的大直径部分。 盖构件（124）可拆卸地安装在壳体（121）的开口的一侧。