公开(公告)号：US20160041072A1

公开(公告)日：2016-02-11

申请号：US14781200

申请日：2014-03-27

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Takuya Yotani ,  Takayuki Oka ,  Hideki Muraki

IPC: G01N1/14 ,  G01N33/49 ,  G01N15/14

CPC classification number: G01N1/14 ,  G01N15/1429 ,  G01N30/16 ,  G01N30/18 ,  G01N33/491 ,  G01N33/4915 ,  G01N35/1016 ,  G01N35/1095 ,  G01N2015/1486 ,  G01N2030/8822

Abstract: Provided is a sample injection device for flow-type analysis including a cylindrical needle (27) which penetrates through an upper wall and a lower wall of a sample injection portion (22) of a carrier-liquid channel through ring-like sealing members (25, 26). The needle (27) includes an inner hole (41) which is closed on a side of a lower end of the needle (27) and open on an outer peripheral surface as a horizontal hole (42). The needle moving unit (44) induces the needle (27) to move downward so that the horizontal hole (42) faces an inside of a sample vessel (40) to draw the sample to the inside of the needle (27). Then the moving unit (44) induces the needle (27) to move upward so that the horizontal hole (42) faces an inside of the sample injection portion (22) to inject the sample in the inside of the needle (27). At an intermediate position, washing liquid is discharged from the horizontal hole (42) of the needle (27), and the washing liquid is recovered via a discharge path (15).

Abstract translation: 本发明提供一种用于流动型分析的样品注射装置，其包括圆柱形针（27），其穿过环状密封构件（25）穿过载液通道的样品注入部分（22）的上壁和下壁 ，26）。 针（27）包括在针（27）的下端侧封闭并且在作为水平孔（42）的外周面上开口的内孔（41）。 针移动单元（44）引导针（27）向下移动，使得水平孔（42）面向样品容器（40）的内部，以将样品抽吸到针（27）的内部。 然后，移动单元（44）引导针（27）向上移动，使得水平孔（42）面向样品注射部分（22）的内部，以将样品注射到针（27）的内部。 在中间位置，洗涤液从针（27）的水平孔（42）排出，洗涤液通过排出路径（15）回收。

公开(公告)号：USD734484S1

公开(公告)日：2015-07-14

申请号：US29480095

申请日：2014-01-23

Applicant: SEKISUI MEDICAL CO., LTD.

Designer： Masato Nishikawa ,  Takayuki Oka ,  Takuya Yotani

公开(公告)号：US12203945B2

公开(公告)日：2025-01-21

申请号：US16971393

申请日：2019-04-18

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Hiroaki Taira ,  Ei-Ichiro Sunamura ,  Kohei Matsuda ,  Takuya Yotani

IPC: G01N33/72 ,  G01N30/02 ,  G01N30/34 ,  G01N30/88 ,  G01N30/96

Abstract: Provided is a method for separating and detecting various hemoglobins including abnormal hemoglobins and thalassemia markers. A method for analyzing hemoglobins, comprising separating hemoglobins in a sample by cation exchange chromatography, wherein an eluent having a pH of 8.1 or more and an osmotic pressure of 40 mOsm/kg or less is used for separation of the hemoglobins.

公开(公告)号：US11142801B2

公开(公告)日：2021-10-12

申请号：US15766631

申请日：2016-10-07

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH ,  SEKISUI MEDICAL CO., LTD.

Inventor： Masami Arai ,  Sachio Nomura ,  Yuriko Nemoto ,  Takuya Yotani

IPC: C12P19/34 ,  C12Q1/6886 ,  G01N30/88 ,  G01N30/02 ,  C12Q1/68 ,  C12N15/09 ,  C12N15/00 ,  C12Q1/6806 ,  B01J20/281

Abstract: It is intended to provide a method for determining a tumor. The method for determining a tumor comprises: (1) treating genomic DNA prepared from a subject tissue or cell with bisulfite (the subject tissue or cell is derived from a patient who is affected by a tumor and is determined as (i) having MSI-H of the tumor in MSI examination and/or no or reduced expression of MLH1 in the tumor in immunohistochemical examination, and (ii) having no mutation in MLH1 in genetic examination); 2) amplifying, by PCR, DNA comprising a portion or the whole of MLH1 promoter region from the bisulfite-treated DNA; 3) subjecting the PCR amplification product to ion exchange chromatography to obtain a detection signal; 4) determining whether or not the peak of the detection signal is a peak indicating highly methylated DNA; and 5) determining the tumor as a tumor derived from a patient without Lynch syndrome when the peak is determined as a peak indicating highly methylated DNA.

公开(公告)号：US10386277B2

公开(公告)日：2019-08-20

申请号：US14908034

申请日：2014-07-25

Applicant: SEKISUI MEDICAL CO., LTD. ,  SEKISUI TECHNO MOLDING CO., LTD.

Inventor： Hiroaki Taira ,  Yuriko Nemoto ,  Takuya Yotani ,  Satoru Tominaga

IPC: G01N1/38 ,  B01L3/00 ,  A61B5/15 ,  G01N1/10 ,  B01F11/00 ,  B01F15/00 ,  G01N35/00

Abstract: A mechanism for collecting a trace of a biological sample to be used for a biological sample analysis, and for preparing a diluted solution of the sample. A container for specimen preparation includes: a biological sample collecting device; and a container for sample dilution. The biological sample collecting device includes: a capillary tube having openings formed at both ends thereof; and a support member for supporting the capillary tube. The container for sample dilution includes: an opening; and a swirl flow generating member formed on an inner wall of the container for sample dilution. The capillary tube of the biological sample collecting device is arranged inside the container for sample dilution by the support member.

公开(公告)号：US09970908B2

公开(公告)日：2018-05-15

申请号：US14905472

申请日：2014-07-17

Applicant: Sekisui Medical Co., Ltd.

Inventor： Takuya Yotani ,  Hiroaki Taira ,  Takayuki Oka ,  Hideki Muraki

IPC: G01N30/34 ,  G01N30/88 ,  G01N30/16 ,  G01N33/49 ,  G01N30/02

CPC classification number: G01N30/34 ,  G01N30/16 ,  G01N30/88 ,  G01N33/491 ,  G01N2030/027 ,  G01N2030/347 ,  G01N2030/8822

Abstract: A gradient liquid feed device allows reduction in size of a high performance liquid chromatography type sample analyzer. The gradient liquid feed device includes a plurality of carrier liquid reservoir tanks configured to store carrier liquids of mutually different compositions, a plurality of single plunger pumps capable of drawing and discharging the carrier liquid from the plurality of carrier liquid reservoir tanks, a mixer configured to mix the carrier liquids discharged from the plurality of liquid feed pumps and feed the mixed carrier liquid, and a pulse damper in communication with the mixer and configured to absorb pulsation that may occur during liquid feeding. The single plunger pumps have a function for variably setting a length of stroke for drawing and discharging the carrier liquid and a function for variably setting a ratio between the carrier liquid drawing time and the carrier liquid discharge time.

公开(公告)号：US09714886B2

公开(公告)日：2017-07-25

申请号：US14357274

申请日：2012-11-09

Applicant: Sekisui Medical Co., Ltd.

Inventor： Takayuki Oka ,  Takuya Yotani ,  Hideki Muraki

IPC: G01N1/18 ,  G01N30/24 ,  G01N1/14 ,  G01N30/18 ,  G01N30/88 ,  G01N30/20

CPC classification number: G01N1/14 ,  G01N30/18 ,  G01N30/20 ,  G01N2030/8822

Abstract: When injecting a sample into carrier-liquid channels (3A and 3B), injection shock is prevented. Septa 13 and 14 constitute the upper wall and the lower wall of a sample injection part (11) of the carrier-liquid channels (3A and 3B). A needle (27) can vertically penetrate the septum (13) on the upper wall side and also penetrate the septum (14) on the lower wall side. A needle moving unit (28) induces the needle (27) to penetrate the septum (14) on the lower wall side and induces the tip of the needle to face the inside of a sample vessel (26). A measurement pump (29) is operated for drawing and as a result a sample is drawn into the needle (27). Next, the needle (27) is extracted from the septum (14) on the lower wall side, the tip of the needle is induced to face the inside of the sample injection part (11), the measurement pump (29) is caused to discharge and as a result the sample within the needle (27) is injected.

公开(公告)号：USD719670S1

公开(公告)日：2014-12-16

申请号：US29480092

申请日：2014-01-23

Applicant: Sekisui Medical Co., Ltd.

Designer： Masato Nishikawa ,  Takayuki Oka ,  Takuya Yotani

公开(公告)号：US10190172B2

公开(公告)日：2019-01-29

申请号：US15121656

申请日：2015-03-02

Applicant: NATIONAL CANCER CENTER ,  SEKISUI MEDICAL CO., LTD.

Inventor： Yae Kanai ,  Eri Arai ,  Yuriko Nemoto ,  Takuya Yotani

IPC: C12Q1/6827 ,  C12Q1/6886 ,  G01N30/96 ,  G01N30/88 ,  B01D15/36

Abstract: It is intended to provide a rapid, convenient, and highly accurate method for determining the prognosis of cancer. The present invention provides a method for determining the prognosis of a renal cell carcinoma patient, comprising: (1) treating genomic DNA prepared from a renal tissue of a subject with bisulfite; (2) amplifying the bisulfite-treated DNA by PCR; (3) subjecting the obtained PCR amplification product to ion exchange chromatography; (4) obtaining the retention time of a detection signal obtained by the chromatography; and (5) determining the renal cell carcinoma of the subject as having poor prognosis when the result of the step (4) is shorter than a retention time serving as a reference.

公开(公告)号：US20160061788A1

公开(公告)日：2016-03-03

申请号：US14786397

申请日：2014-04-22

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Takuya Yotani ,  Hiroaki Taira ,  Takayuki Oka ,  Hideki Muraki

IPC: G01N30/20 ,  G01N33/49

CPC classification number: G01N30/20 ,  G01N33/491 ,  G01N35/1097 ,  G01N2030/201 ,  G01N2030/202 ,  G01N2030/207

Abstract: A switching valve includes: (A) a rotor including: (1) a center pipe connection port, (2) a first in-valve flow path in communication with the center pipe connection port, and (3) an arc-like second in-valve flow path; (B) a stator including: (4) a first pipe connection port group which is brought into communication independently with the center pipe connection port via the first in-valve flow path when the rotor is turned, and (5) a second pipe connection port group which is brought into mutual communication via the second in-valve flow path when the rotor is turned; and (C) an arrangement of the rotor and the stator satisfying the following relationship: the state of communication or non-communication among the second pipe connection port group via the second in-valve flow path is switched in accordance with the state of communication between the first pipe connection port group and the center pipe connection port.

Abstract translation: 一种切换阀，包括：（A）转子，包括：（1）中心管连接口，（2）与中心管连接口连通的第一阀门流路，（3）弧形第二 阀门流路; （B）定子，包括：（4）第一管连接端口组，当所述转子转动时，所述第一管连接端口组经由所述第一阀内流路与所述中心管连接口独立连通，以及（5）第二管连接 端子组，当转子旋转时，通过第二入口流路相互连通; 和（C）转子和定子的布置满足以下关系：根据第二管连接端口组之间的通过第二管路连通口组的通信状态或非连通状态根据第 第一个管道连接端口组和中心管道连接端口。