公开(公告)号：US20150344854A1

公开(公告)日：2015-12-03

申请号：US14828839

申请日：2015-08-18

申请人： Codexis, Inc.

发明人： Jovana Nazor ,  Derek Smith ,  Michael Crowe ,  Shiwei Song ,  Steven J. Collier

IPC分类号： C12N9/10

CPC分类号： C12N9/1096 ,  C12P17/10 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

摘要： The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

摘要翻译： 本公开提供了用于制备胺的工程化转氨酶多肽，编码工程改造氨基酸的多核苷酸，能够表达工程化转氨酶的宿主细胞以及使用工程改造的转氨酶来制备用于生产活性药剂的化合物的方法。

公开(公告)号：US09139821B2

公开(公告)日：2015-09-22

申请号：US14386082

申请日：2013-03-22

申请人： Codexis, Inc.

发明人： Jovana Nazor ,  Derek Smith ,  Michael Crowe ,  Shiwei Song ,  Steven J. Collier

IPC分类号： C12P17/10 ,  C12N9/10

CPC分类号： C12N9/1096 ,  C12P17/10 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

摘要： The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the making the polypeptides, and methods of using the polypeptides for the biocatalytic conversion of ketone substrates to amine products. The present enzymes have been engineered to have one or more residue differences as compared to the amino acid sequence of the naturally occurring transaminase of Vibrio fluvialis. In particular, the transaminases of the present disclosure have been engineered for efficient formation of chiral tryptamine derivatives from its corresponding prochiral ketone substrates.

摘要翻译： 本公开提供了用于制备胺的工程化转氨酶多肽，编码工程改造氨基酸的多核苷酸，能够表达工程化转氨酶的宿主细胞以及使用工程改造的转氨酶来制备用于生产活性药剂的化合物的方法。 本公开提供了具有转氨酶活性的工程改造的多肽，编码多肽的多核苷酸，制备多肽的方法，以及使用多肽将酮底物生物催化转化成胺产物的方法。 与本发明的弧菌弧菌的天然存在的转氨酶的氨基酸序列相比，本发明的酶已被改造成具有一个或多个残基差异。 特别地，本公开的转氨酶已被设计用于从其相应的前手性酮底物有效形成手性色胺衍生物。

公开(公告)号：US20150210988A1

公开(公告)日：2015-07-30

申请号：US14684916

申请日：2015-04-13

申请人： Codexis Inc.

发明人： Ish K. Dhawan ,  Gregory Miller ,  Xiyun Zhang

IPC分类号： C12N9/10

CPC分类号： C12N9/1096 ,  C12P13/001 ,  C12P41/006 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

摘要： The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

摘要翻译： 本公开提供与天然存在的野生型转氨酶相比具有改进性质的工程化转氨酶。 还提供了编码工程改造的转氨酶的多核苷酸，能够表达工程化转氨酶的宿主细胞以及使用工程改造的转氨酶合成各种手性化合物的方法。

公开(公告)号：US09029106B2

公开(公告)日：2015-05-12

申请号：US13920902

申请日：2013-06-18

申请人： Codexis Inc.

发明人： Ish K. Dhawan ,  Gregory Miller ,  Xiyun Zhang

IPC分类号： C12N9/10 ,  C12P13/00 ,  C12P41/00

CPC分类号： C12N9/1096 ,  C12P13/001 ,  C12P41/006 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

摘要： The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

摘要翻译： 本公开提供与天然存在的野生型转氨酶相比具有改进性质的工程化转氨酶。 还提供了编码工程改造的转氨酶的多核苷酸，能够表达工程化转氨酶的宿主细胞以及使用工程改造的转氨酶合成各种手性化合物的方法。

公开(公告)号：US20150125480A1

公开(公告)日：2015-05-07

申请号：US14537599

申请日：2014-11-10

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： YVONNE PATERSON ,  THORSTEN VERCH

IPC分类号： A61K39/12 ,  A61K39/00 ,  C12N15/74 ,  C12N7/00

CPC分类号： A61K39/0011 ,  A61K35/74 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/585 ,  C12N7/00 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N2710/00034 ,  C12N2710/20033 ,  C12Y206/01 ,  C12Y206/01021 ,  C12Y501/01001

摘要： The present invention provides Listeria vaccine strains that express a heterologous antigen and a metabolic enzyme, and methods of generating same.

摘要翻译： 本发明提供表达异源抗原和代谢酶的利斯特氏菌疫苗株及其产生方法。

公开(公告)号：US20140199737A1

公开(公告)日：2014-07-17

申请号：US14139225

申请日：2013-12-23

申请人： INVISTA North America S.á r.l.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC分类号： C12P13/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/00 ,  C12P7/44

CPC分类号： C12P13/005 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/86 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/02 ,  C12Y206/01 ,  C12Y301/02

摘要： This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.

摘要翻译： 本文件描述了通过在羧基，胺或羟基中形成一个或两个末端官能团来生产己二酸，6-氨基己酸，6-羟基己酸，六亚甲基二胺，己内酰胺或1,6-己二醇的生化途径 C6脂肪族主链基质。 本文描述的这些途径，代谢工程和培养策略依赖于接受甲酯屏蔽二羧酸底物的酶或同系物。

公开(公告)号：US20140199734A1

公开(公告)日：2014-07-17

申请号：US13810004

申请日：2011-07-14

申请人： Martin Schürmann ,  Wijnand Peter Helena Peeters ,  Natascha Hubertina Smeets ,  Helmut Schwab ,  Kerstin Steiner ,  Kateryna Mykoiayivna Lypetska ,  Gernot Strohmeier

发明人： Martin Schürmann ,  Wijnand Peter Helena Peeters ,  Natascha Hubertina Smeets ,  Helmut Schwab ,  Kerstin Steiner ,  Kateryna Mykoiayivna Lypetska ,  Gernot Strohmeier

IPC分类号： C12N9/10 ,  C12P13/00

CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12Y206/01

摘要： The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.

摘要翻译： 本发明涉及通过使用新型转氨酶从通式[1] [a]的相应酮酶促合成通式[1] [c]的对映异构体富集的（R） - 胺的方法。 这些新型转氨酶选自两个不同的组：来自约20个具有本文所述序列的蛋白质的组，或来自具有转氨酶活性的一组蛋白质，并且从选自以下的微生物中分离：微生物，其由Rahnella aquatilis，Ochrobactrum anthropi，苍白杆菌小麦，苜蓿morelense，Curtobacterium属pusilllum，淡紫拟青霉，微ginsengisoli，微trichothecenolyticum，假单胞菌citronellolis，耶尔森kristensenii，无色spanius，无色insolitus，偶发分枝杆菌，结核分枝frederiksbergense，结核分枝骶骨，结核分枝fluoranthenivorans，鼻疽菌，类鼻疽tropica ，天蛾孢子和尖孢镰刀菌（Fusarium oxysporum）。

公开(公告)号：US20140193863A1

公开(公告)日：2014-07-10

申请号：US14138971

申请日：2013-12-23

申请人： INVISTA North America S.á r.I.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Changlin Chen ,  Paul S. Pearlman

IPC分类号： C12P13/00 ,  C12P7/42 ,  C12P7/18 ,  C12P7/44

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01 ,  C12Y101/01085 ,  C12Y101/01087 ,  C12Y101/01258 ,  C12Y101/01286 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y102/0101 ,  C12Y102/99006 ,  C12Y203/01032 ,  C12Y203/03013 ,  C12Y203/03014 ,  C12Y206/01 ,  C12Y207/08 ,  C12Y208/03012 ,  C12Y301/02 ,  C12Y305/01062 ,  C12Y401/01043 ,  C12Y402/01033 ,  C12Y402/01036 ,  C12Y402/01114 ,  C12Y602/01005

摘要： This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.

摘要翻译： 本文件描述了通过在C7脂肪族中形成一个或两个由羧基，胺或羟基组成的末端官能团，生成庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇的生化途径 骨架底物。 本文描述的这些途径，代谢工程和培养策略依赖于C1延长酶或与辅酶B生物合成相关的同源物。

公开(公告)号：US20140193861A1

公开(公告)日：2014-07-10

申请号：US14138827

申请日：2013-12-23

申请人： INVISTA North America S.á.r.l.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie ,  Changlin Chen ,  Paul S. Pearlman

IPC分类号： C12P13/00 ,  C12P7/42 ,  C12P7/18 ,  C12P7/44

CPC分类号： C12P7/44 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/80 ,  C12N9/93 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01258 ,  C12Y102/01063 ,  C12Y206/01 ,  C12Y208/03012 ,  C12Y301/02 ,  C12Y305/01062 ,  C12Y602/01005

摘要： This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-CoA degradation pathway enzymes.

摘要翻译： 本文件描述了通过在C7脂肪族主链基质中形成由羧基，胺或羟基组成的两个末端官能团，生成庚二酸，7-氨基庚酸酯，7-羟基庚酸酯，七亚甲基二胺或1,7-庚二醇的生化途径， 草酸盐或苯甲酸盐。 本文描述的这些途径，代谢工程和培养策略依赖于厌氧苯甲酰辅酶A降解途径酶。

公开(公告)号：US08592189B2

公开(公告)日：2013-11-26

申请号：US13525129

申请日：2012-06-15

申请人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

发明人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC分类号： C12P7/44 ,  C12N1/20

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

摘要： The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

摘要翻译： 本发明提供了具有己二酸，6-氨基己酸或己内酰胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应己二酸，6-氨基己酸或己内酰胺途径中的酶的外源核酸。 本发明另外提供了生产己二酸，6-氨基己酸或己内酰胺的方法。 该方法可以包括培养己二酸，6-氨基己酸或己内酰胺生产微生物，其中微生物生物体表达至少一种编码己二酸，6-氨基己酸或己内酰胺途径酶的外源核酸，其量足以产生相应的产物 在条件下和足够的时间内生产己二酸，6-氨基己酸或己内酰胺。