知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

74 条记录 (耗时 0.066 秒)

    METHODS FOR INCREASING PRODUCT YIELDS
    5.
    发明申请
    METHODS FOR INCREASING PRODUCT YIELDS 有权
    增加产品线的方法

    公开(公告)号：US20110201089A1

    公开(公告)日：2011-08-18

    申请号：US13011796

    申请日：2011-01-21

    申请人： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    发明人： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC分类号： C12N1/00

    CPC分类号： C12P7/00 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0095 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/42 ,  C12P19/42

    摘要： A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    摘要翻译： 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间段内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为结构单元的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE
    6.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE 有权
    生产环己酮的有机体

    公开(公告)号：US20110014668A1

    公开(公告)日：2011-01-20

    申请号：US12780802

    申请日：2010-05-14

    申请人： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    发明人： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    IPC分类号： C12P7/26 ,  C12N1/00

    CPC分类号： C12P7/26 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70

    摘要： A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

    摘要翻译： 非天然存在的微生物有机体具有包含至少一种编码环己酮途径酶的外源核酸的环己酮途径。 途径包括2-酮环己烷-1-羧基-CoA水解酶（作用于C-C键），2-酮环己烷-1-羧酸脱羧酶和选自2-酮环己烷-1-羧基-CoA水解酶的酶（作用 在硫酯上），2-酮环己烷-1-羧基-CoA转移酶和2-酮环己烷-1-羧基-CoA合成酶。 途径包括选自6-酮环己-1-烯-1-羧基-CoA水解酶（作用于C-C键）的酶，6-酮环己-1-烯-1-羧基-CoA合成酶，6- 酮酮环己-1-烯-1-羧基-CoA水解酶（作用于硫酯），6-酮环己-1-烯-1-羧基-CoA转移酶，6-酮环己-1-烯-1-羧基-CoA还原酶， 6-酮环己-1-烯-1-羧酸脱羧酶，6-酮环己-1-烯-1-羧酸还原酶，2-酮环己烷-1-羧基-CoA合成酶，2-酮环己烷-1-羧基-CoA 转移酶，2-酮环己烷-1-羧基-CoA水解酶（作用于硫酯），2-酮环己烷-1-羧酸脱羧酶和环己酮脱氢酶。 途径包括己二酸半醛脱水酶，环己烷-1,2-二醇脱氢酶和环己烷-1,2-二醇脱水酶。 途径包括3-氧代邻苯二甲酸脱羧酶，4-乙酰基丁酸脱水酶，3-羟基环己酮脱氢酶，2-环己烯酮水合酶，环己酮脱氢酶和选自3-氧代木糖酰辅酶A合成酶，3-氧代木糖酰-CoA水解酶 （作用于硫酯）和3-氧代木糖酰辅酶A转移酶。 每个这些途径可以包括PEP羧基激酶。 生产环己酮的方法包括培养这些非天然存在的微生物。

    Microorganisms for the production of adipic acid and other compounds
    7.
    发明授权
    Microorganisms for the production of adipic acid and other compounds 有权
    用于生产己二酸等化合物的微生物

    公开(公告)号：US07799545B2

    公开(公告)日：2010-09-21

    申请号：US12413355

    申请日：2009-03-27

    申请人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    发明人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    IPC分类号： C12P7/40 ,  C12N1/20

    CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

    摘要： The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

    摘要翻译： 本发明提供了具有己二酸，6-氨基己酸或己内酰胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应己二酸，6-氨基己酸或己内酰胺途径中的酶的外源核酸。 本发明另外提供了生产己二酸，6-氨基己酸或己内酰胺的方法。 该方法可以包括培养己二酸，6-氨基己酸或己内酰胺生产微生物，其中微生物生物体表达至少一种编码己二酸，6-氨基己酸或己内酰胺途径酶的外源核酸，其量足以产生相应的产物 在条件下和足够的时间内生产己二酸，6-氨基己酸或己内酰胺。

    Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
    8.
    发明授权
    Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene 有权
    用于生物合成芳烃，2,4-戊二烯酸和1,3-丁二烯的微生物和方法

    公开(公告)号：US08715957B2

    公开(公告)日：2014-05-06

    申请号：US13191375

    申请日：2011-07-26

    申请人： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

    发明人： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

    IPC分类号： C12P1/00 ,  A61K38/00

    CPC分类号： C12P7/62 ,  C12N15/52 ,  C12P5/005 ,  C12P5/026 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  Y02E50/10

    摘要： The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

    摘要翻译： 本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯途径。 本发明另外提供了使用这些生物体来生产甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯。

    MICROORGANISMS FOR PRODUCING 1,4-BUTANEDIOL AND METHODS RELATED THERETO
    10.
    发明申请
    MICROORGANISMS FOR PRODUCING 1,4-BUTANEDIOL AND METHODS RELATED THERETO 审中-公开
    用于生产1,4-丁二醇的微生物及其相关方法

    公开(公告)号：US20130034884A1

    公开(公告)日：2013-02-07

    申请号：US13530053

    申请日：2012-06-21

    申请人： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    发明人： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC分类号： C12N1/21 ,  C12N9/02 ,  C12P17/04 ,  C12N1/19 ,  C12P7/18 ,  C12P7/42

    CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12P7/16 ,  C12P7/18 ,  C12P7/42 ,  C12Y102/99006 ,  Y02E50/10

    摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

    摘要翻译： 本发明提供了包含1,4-丁二醇（BDO），4-羟基丁酰辅酶A，4-羟基丁醛或腐胺途径的非天然存在的微生物，其包含至少一种编码BDO，4-羟基丁酰辅酶A， 表达量足以产生BDO，4-羟基丁酰辅酶A，4-羟基丁醛或腐胺的β-羟基丁酸或腐胺途径酶，并进一步优化BDO的表达。 本发明另外提供了使用这些微生物生产BDO，4-羟基丁酰辅酶A，4-羟基丁醛或腐胺的方法。