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131 条记录 (耗时 0.019 秒)

    Process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids
    4.
    发明授权
    Process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids 失效
    将由微生物产生的类胡萝卜素转化为氰orr烯的方法

    公开(公告)号：US5026640A

    公开(公告)日：1991-06-25

    申请号：US657748

    申请日：1984-10-04

    申请人： Agnes Kelemen ,  Eva Cserey Pechany ,  Istvan Jaksa

    发明人： Agnes Kelemen ,  Eva Cserey Pechany ,  Istvan Jaksa

    IPC分类号： C07D487/22 ,  C07H23/00 ,  C12P17/18 ,  C12P19/42 ,  C12R1/01 ,  C12R1/15 ,  C12R1/225

    CPC分类号： C07H23/00 ,  C12P17/182 ,  C12P19/42

    摘要： The invention relates to a process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids by reaction with cyanides. According to the invention a fermentation broth obtained by disruption of microorganism cells in a known manner, preferably by heat treatment in the presence or absence of sulfite ions, optionally after purification steps known per se, is contacted with a suitable adsorbent or ion exchange resin, the corrinoids adsorbed on the surface of the adsorbent or ion exchange resin are treated with an aqueous solution containing cyanide ions or a compound capable of supplying cyanide ions, in an amount providing cyanide ions in a 1.1 to 2.0-fold molar excess related to the corrinoids, the adsorbent or ion exchange resin is washed with water, and the cyanocorrinoids obtained is eluted in a known manner, preferably with aqueous ethanol.

    摘要翻译： 本发明涉及通过与氰化物反应将由微生物产生的类胡萝卜素转化成氰orr烯的方法。 根据本发明，通过以已知方式破坏微生物细胞获得的发酵液，优选通过在存在或不存在亚硫酸根离子的情况下，任选地在本身已知的纯化步骤之后进行热处理，与合适的吸附剂或离子交换树脂接触， 吸附在吸附剂或离子交换树脂表面上的类胡萝卜素用含氰化物离子或能够提供氰化物离子的化合物的水溶液进行处理，其量为氰化物离子的量为1.1-2.0倍摩尔过量，与脯氨酸相关 吸附剂或离子交换树脂用水洗涤，所得的氰肟酮以已知的方式，优选用含水乙醇洗脱。

    Process for fermentatively producing vitamin B.sub.12
    5.
    发明授权
    Process for fermentatively producing vitamin B.sub.12 失效
    发酵生产维生素B的方法（HD 12

    公开(公告)号：US4119492A

    公开(公告)日：1978-10-10

    申请号：US763539

    申请日：1977-01-28

    申请人： Ichiro Kojima ,  Hiroshi Sato ,  Yasuo Fujiwara

    发明人： Ichiro Kojima ,  Hiroshi Sato ,  Yasuo Fujiwara

    IPC分类号： C12N1/32 ,  C12P19/42 ,  C12R1/06 ,  C12D5/06

    CPC分类号： C12P19/42 ,  C12N1/32 ,  Y10S435/83

    摘要： A process for producing vitamin B.sub.12, which comprises cultivating a vitamin B.sub.12 -producing micro-organism of the genus Arthrobacter under aerobic conditions in a culture medium containing at least one compound selected from the group consisting of alcohols with 2 or 3 carbon atoms and ketones, and separating vitamin B.sub.12 from the culture broth.

    摘要翻译： 一种生产维生素B12的方法，其包括在需氧条件下，在含有至少一种选自具有2或3个碳原子的醇和酮的化合物的培养基中培养节杆菌属的维生素B12生产微生物， 并从培养液中分离维生素B12。

    Process for the production of fermentation broth with increased vitamin B.sub.12 content by synchronizing the bacterium population
    6.
    发明授权
    Process for the production of fermentation broth with increased vitamin B.sub.12 content by synchronizing the bacterium population 失效
    生产具有增加维生素B的发酵肉汤的方法{HD 12 {通过同步细菌群体的B含量

    公开(公告)号：US3979259A

    公开(公告)日：1976-09-07

    申请号：US517788

    申请日：1974-10-24

    申请人： Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

    发明人： Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

    IPC分类号： C12N1/32 ,  C12P5/02 ,  C12P19/42 ,  C12R1/01 ,  C12D5/06

    CPC分类号： C12P5/023 ,  C12P19/42 ,  Y02E50/343 ,  Y10S435/801 ,  Y10S435/802 ,  Y10S435/813

    摘要： The invention relates to a process for the production of a fermentation broth with increased vitamin B.sub.12 content by a fermentation process carried out with a methane-producing mixed bacterium population under anaerobic septic conditions in the presence of known nutrient components and precursors. According to the invention, methanol, preferably in an amount of 0.5 v/v %, is added for some days, preferably for 3 days to the fermentation broth containing vitamin B.sub.12 used as the starting substance. Thereafter a minor part, preferably 10% of the fermentation broth, is removed and an equal volume of a nutrient broth containing the usual components in tenfold concentrations is added periodically in every 5th to 12th day, then the removal of fermentation broth is interrupted for 0 to 2 days, preferably for 1 day, and only 0.4 to 1.5 v/v% of methanol are added to the fermentation broth, and subsequently a minor part, preferably 10% of the fermentation broth, is removed daily and an equal volume of a nutrient broth containing the usual components in the usual concentrations is added together with 0.4 to 1.5 v/v % of methanol, depending on the biogas production of the fermentation broth. This series of operations is repeated periodically during the complete fermentation procedure taking into consideration the pH of the fermentation broth.

    摘要翻译： 本发明涉及通过在已知营养成分和前体的存在下在厌氧脓毒症条件下用产甲烷混合菌群进行的发酵方法来生产具有增加的维生素B12含量的发酵液的方法。 根据本发明，优选以0.5v / v％的量将甲醇加入含有用作起始物质的维生素B12的发酵液中几天，优选3天。 此后，除去少量的发酵肉汤，并且每5至12天定期添加等体积的含有十倍浓度的通常组分的营养肉汤，然后将发酵肉汤的去除中断0 至2天，优选1天，并且仅将0.4至1.5v / v％的甲醇加入到发酵液中，随后每天除去少部分，优选10％的发酵液，并加入等体积的 根据发酵液的生物气产量，含有通常浓度的通常组分的营养肉汤与0.4至1.5v / v％的甲醇一起加入。 考虑到发酵液的pH值，在整个发酵过程中定期重复这一系列操作。