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    MEANS AND METHODS FOR VITAMIN B12 PRODUCTION IN DUCKWEED
    1.
    发明申请
    MEANS AND METHODS FOR VITAMIN B12 PRODUCTION IN DUCKWEED 审中-公开

    公开(公告)号：US20200239922A1

    公开(公告)日：2020-07-30

    申请号：US16841770

    申请日：2020-04-07

    Applicant: Hinoman Ltd.

    Inventor： Vladimir GLUKHMAN ,  Ehud ELITUV

    IPC: C12P19/42 ,  A01H5/00 ,  A01H4/00 ,  A01H3/00 ,  C12N1/20

    Abstract: The present invention is directed to a system and method for producing a vitamin B12 enriched Duckweed Bacterial Culture (DBC) composition. The present invention further discloses a composition comprising a vitamin B12 enriched DBC, wherein said composition comprises at least one Lemnoideae species and at least one vitamin B12 producing bacteria species, further wherein said vitamin B12 content in said composition is in the range of between about 0.01 and about 100 μg per 100 g of said DBC.

    Genes from propionibacterium freudenreichii encoding enzymes involved in vitamin b12 biosynthesis
    7.
    发明申请
    Genes from propionibacterium freudenreichii encoding enzymes involved in vitamin b12 biosynthesis 失效
    编码参与维生素B12生物合成的酶的丙酸杆菌属的基因

    公开(公告)号：US20060183188A1

    公开(公告)日：2006-08-17

    申请号：US10522389

    申请日：2003-07-25

    Applicant: Herman Pel ,  Sylvia Hopper

    Inventor： Herman Pel ,  Sylvia Hopper

    IPC: C12P19/42 ,  C12P17/00 ,  C12P13/00 ,  C12P9/00 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10

    CPC classification number: C12N9/93 ,  C12N9/1085 ,  C12N9/1241 ,  C12N9/1288

    Abstract: Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encoding the four enzymes can be placed in Propionibacteria shuttle vectors and used to transform Propionibacteria hosts, in order to improve the production of vitamin B12 or a precursor thereof on an industrial scale during fermentation.

    Abstract translation: 公开了四种新基因及其编码的酶，它们存在于丙酸杆菌中，并且在维生素B 12的生物合成途径中参与至少五个步骤。 四种酶是：A：豆油酸a，c-二酰胺合酶; B：双功能酶，它是一种辅酶酰胺激酶和一种胞嘧啶酰胺磷酸鸟嘌呤转移酶; C：钴胺素5-磷酸合成酶; 和D：腺苷酰基转移酶。 编码四种酶的基因可以放置在丙酸杆菌穿梭载体中，并用于转化丙酸杆菌宿主，以便在发酵期间以工业规模提高维生素B 12 /或其前体的产生。

    Process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids
    8.
    发明授权
    Process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids 失效
    将由微生物产生的类胡萝卜素转化为氰orr烯的方法

    公开(公告)号：US5026640A

    公开(公告)日：1991-06-25

    申请号：US657748

    申请日：1984-10-04

    Applicant: Agnes Kelemen ,  Eva Cserey Pechany ,  Istvan Jaksa

    Inventor： Agnes Kelemen ,  Eva Cserey Pechany ,  Istvan Jaksa

    IPC: C07D487/22 ,  C07H23/00 ,  C12P17/18 ,  C12P19/42 ,  C12R1/01 ,  C12R1/15 ,  C12R1/225

    CPC classification number: C07H23/00 ,  C12P17/182 ,  C12P19/42

    Abstract: The invention relates to a process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids by reaction with cyanides. According to the invention a fermentation broth obtained by disruption of microorganism cells in a known manner, preferably by heat treatment in the presence or absence of sulfite ions, optionally after purification steps known per se, is contacted with a suitable adsorbent or ion exchange resin, the corrinoids adsorbed on the surface of the adsorbent or ion exchange resin are treated with an aqueous solution containing cyanide ions or a compound capable of supplying cyanide ions, in an amount providing cyanide ions in a 1.1 to 2.0-fold molar excess related to the corrinoids, the adsorbent or ion exchange resin is washed with water, and the cyanocorrinoids obtained is eluted in a known manner, preferably with aqueous ethanol.

    Abstract translation: 本发明涉及通过与氰化物反应将由微生物产生的类胡萝卜素转化成氰orr烯的方法。 根据本发明，通过以已知方式破坏微生物细胞获得的发酵液，优选通过在存在或不存在亚硫酸根离子的情况下，任选地在本身已知的纯化步骤之后进行热处理，与合适的吸附剂或离子交换树脂接触， 吸附在吸附剂或离子交换树脂表面上的类胡萝卜素用含氰化物离子或能够提供氰化物离子的化合物的水溶液进行处理，其量为氰化物离子的量为1.1-2.0倍摩尔过量，与脯氨酸相关 吸附剂或离子交换树脂用水洗涤，所得的氰肟酮以已知的方式，优选用含水乙醇洗脱。

    Process for fermentatively producing vitamin B.sub.12
    9.
    发明授权
    Process for fermentatively producing vitamin B.sub.12 失效
    发酵生产维生素B的方法（HD 12

    公开(公告)号：US4119492A

    公开(公告)日：1978-10-10

    申请号：US763539

    申请日：1977-01-28

    Applicant: Ichiro Kojima ,  Hiroshi Sato ,  Yasuo Fujiwara

    Inventor： Ichiro Kojima ,  Hiroshi Sato ,  Yasuo Fujiwara

    IPC: C12N1/32 ,  C12P19/42 ,  C12R1/06 ,  C12D5/06

    CPC classification number: C12P19/42 ,  C12N1/32 ,  Y10S435/83

    Abstract: A process for producing vitamin B.sub.12, which comprises cultivating a vitamin B.sub.12 -producing micro-organism of the genus Arthrobacter under aerobic conditions in a culture medium containing at least one compound selected from the group consisting of alcohols with 2 or 3 carbon atoms and ketones, and separating vitamin B.sub.12 from the culture broth.

    Abstract translation: 一种生产维生素B12的方法，其包括在需氧条件下，在含有至少一种选自具有2或3个碳原子的醇和酮的化合物的培养基中培养节杆菌属的维生素B12生产微生物， 并从培养液中分离维生素B12。

    Process for the production of fermentation broth with increased vitamin B.sub.12 content by synchronizing the bacterium population
    10.
    发明授权
    Process for the production of fermentation broth with increased vitamin B.sub.12 content by synchronizing the bacterium population 失效
    生产具有增加维生素B的发酵肉汤的方法{HD 12 {通过同步细菌群体的B含量

    公开(公告)号：US3979259A

    公开(公告)日：1976-09-07

    申请号：US517788

    申请日：1974-10-24

    Applicant: Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

    Inventor： Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

    IPC: C12N1/32 ,  C12P5/02 ,  C12P19/42 ,  C12R1/01 ,  C12D5/06

    CPC classification number: C12P5/023 ,  C12P19/42 ,  Y02E50/343 ,  Y10S435/801 ,  Y10S435/802 ,  Y10S435/813

    Abstract: The invention relates to a process for the production of a fermentation broth with increased vitamin B.sub.12 content by a fermentation process carried out with a methane-producing mixed bacterium population under anaerobic septic conditions in the presence of known nutrient components and precursors. According to the invention, methanol, preferably in an amount of 0.5 v/v %, is added for some days, preferably for 3 days to the fermentation broth containing vitamin B.sub.12 used as the starting substance. Thereafter a minor part, preferably 10% of the fermentation broth, is removed and an equal volume of a nutrient broth containing the usual components in tenfold concentrations is added periodically in every 5th to 12th day, then the removal of fermentation broth is interrupted for 0 to 2 days, preferably for 1 day, and only 0.4 to 1.5 v/v% of methanol are added to the fermentation broth, and subsequently a minor part, preferably 10% of the fermentation broth, is removed daily and an equal volume of a nutrient broth containing the usual components in the usual concentrations is added together with 0.4 to 1.5 v/v % of methanol, depending on the biogas production of the fermentation broth. This series of operations is repeated periodically during the complete fermentation procedure taking into consideration the pH of the fermentation broth.

    Abstract translation: 本发明涉及通过在已知营养成分和前体的存在下在厌氧脓毒症条件下用产甲烷混合菌群进行的发酵方法来生产具有增加的维生素B12含量的发酵液的方法。 根据本发明，优选以0.5v / v％的量将甲醇加入含有用作起始物质的维生素B12的发酵液中几天，优选3天。 此后，除去少量的发酵肉汤，并且每5至12天定期添加等体积的含有十倍浓度的通常组分的营养肉汤，然后将发酵肉汤的去除中断0 至2天，优选1天，并且仅将0.4至1.5v / v％的甲醇加入到发酵液中，随后每天除去少部分，优选10％的发酵液，并加入等体积的 根据发酵液的生物气产量，含有通常浓度的通常组分的营养肉汤与0.4至1.5v / v％的甲醇一起加入。 考虑到发酵液的pH值，在整个发酵过程中定期重复这一系列操作。

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