公开(公告)号：US09598708B2

公开(公告)日：2017-03-21

申请号：US14525549

申请日：2014-10-28

申请人： Robert Hickey ,  Richard E. Tobey ,  Shih-Perng Tsai

发明人： Robert Hickey ,  Richard E. Tobey ,  Shih-Perng Tsai

IPC分类号： C12P7/54 ,  C12P7/06 ,  C12M1/04 ,  C12M1/36 ,  C12P7/16 ,  C12P7/02 ,  C12P7/52 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12P7/54 ,  C12M29/06 ,  C12M41/32 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/065 ,  C12P7/16 ,  C12P7/52 ,  Y02E50/10 ,  Y02E50/17 ,  Y02P20/582 ,  Y10S435/801

摘要： Processes for starting up and operating anaerobic, deep tank fermentation systems to anaerobically bioconvert hydrogen and carbon monoxide in a gaseous substrate stream to oxygenated organic compounds and for steady operation of such fermentation systems are shown. Injectors use a motive liquid to introduce gas substrate as a stable gas-in liquid dispersion into the deep tank fermentation reactor where at least one of:(i) adjusting the gas to liquid flow ratio through an injector,(ii) changing the rate of liquid flow through an injector, and(iii) adjusting the carbon monoxide mole fraction in the gas feed by admixture with at least one other gas,wherein the mass transfer of carbon monoxide to an aqueous menstruum in the reactor is controlled to obtain the robust growth of the microorganism and/or continued conversion of gas substrate while maintaining the carbon monoxide concentration below that amount which is unduly adverse to the microorganism.

公开(公告)号：US20070221572A1

公开(公告)日：2007-09-27

申请号：US11587029

申请日：2005-04-18

申请人： Giuseppe Natta

发明人： Giuseppe Natta

IPC分类号： C02F3/30

CPC分类号： B09B3/00 ,  B03B9/06 ,  C10L3/08 ,  C10L5/46 ,  F23G5/02 ,  F23G2201/601 ,  F23G2201/602 ,  F23G2201/80 ,  F23G2206/202 ,  F23G2206/203 ,  F23G2900/50208 ,  F23G2900/50209 ,  Y02E20/12 ,  Y02E50/10 ,  Y02E50/30 ,  Y10S435/801 ,  Y10S435/819

摘要： The method aims at obtaining from waste and more particularly from municipal solid waste (MSW) the energy contained therein at the highest level for industrial use by means of natural technologies and with low environmental impact. The method, denoted by the acronym NEW (Natural Energy from Waste) operates through the following process phases: a) aerobic digestion of the putrescible biological part to produce stabilised waste which is easy to handle, b) separation of a fraction rich in materials with a high heat value, c) storage of the residue, rich in biodegradable and inert substances, compacted into appropriate geometrical shapes in bioreactors which can be activated and sealed, d) activation of the bioreactors with water and their service in time during anaerobic digestion to supply biogas to be used for the production of energy, e) bio-stabilisation and dehydration of the residual material of the anaerobic treatment with air, f) possible recovery of the materials produced in this way. In this way the energy contained in the waste, is extracted at the most refined level in the form of plastic, plastic/paper and methane for energy uses with maximum yield and reduced production of ash, and the end material leaving the bioreactors is fully exhausted of its energy content and inertised.

摘要翻译： 该方法旨在从废物，特别是城市固体废物（MSW）获得最高水平的能源，通过自然技术和环境影响较小的工业用途。 由简称NEW（废物自然能源）表示的方法通过以下过程阶段进行操作：a）对不可逆生物部分进行有氧消化以产生易于处理的稳定化废物，b）将富含材料的馏分分离成 高热值，c）残留物的储存，富含可生物降解和惰性物质，在可以活化和密封的生物反应器中压实成适当的几何形状，d）在厌氧消化期间及时使用生物反应器的活化及其使用 供应用于生产能源的沼气，e）用空气对厌氧处理的残余物质进行生物稳定和脱水，f）以这种方式生产的材料的可能回收。 以这种方式，包含在废物中的能量以塑料，塑料/纸和甲烷的形式在最精炼的水平下被提取，用于能量使用，具有最大的产量和减少灰分的产生，并且离开生物反应器的最终材料被完全耗尽 的能量含量和惰性。

公开(公告)号：US06265210B1

公开(公告)日：2001-07-24

申请号：US09254754

申请日：1999-03-08

申请人： Peter Silley ,  Michael John Annable

发明人： Peter Silley ,  Michael John Annable

IPC分类号： C12M100

CPC分类号： C12M41/34 ,  B25J21/02 ,  Y10S435/801 ,  Y10S435/809

摘要： Controlled atmosphere cabinet has enclosure (110;210) with means providing visibility (110F; 210F) of its interior from outside, gas supply (113-116, 212-216) for desired or target composition of internal atmosphere, controlled lock-type access (112) for manual introduction and removal of items, and hand/arm access port provision (111A, B; 211A, B) for manipulation of said samples in the cabinet. Gas supply associated control means (230-240) serves during use of the cabinet (other than normal atmosphere augmenting in use of the lock-type access) for additional gas supply further to correct or compensate for effects actually or potentially adverse to maintaining the desired or target internal atmosphere composition, via operator-actuated input means (121t, 123; 222) and/or means (122, 124, 139; 225) for sensing prescribed conditions to which the control means is responsive for said additional gas supply.

摘要翻译： 受控气氛柜具有外壳（110; 210），其具有从外部提供其内部的可视性（110F; 210F）的装置，用于期望的或目标组成的内部气氛的气体供应（113-116,212-216），受控的锁式接入 （112），用于手动引入和移除物品，以及用于操纵柜中的所述样品的手/臂进出口提供（111A，B; 211A，B）。 气体供应相关的控制装置（230-240）在柜​​的使用期间（除了使用锁式通道的正常大气增加之外）用于额外的气体供应，以进一步校正或补偿实际上或潜在地不利于维持期望的 或目标内部气氛组成，经由操作者致动的输入装置（121t，123; 222）和/或用于感测控制装置对所述附加气体供应响应的规定条件的装置（122,124,139; 225）。

公开(公告)号：US06255080B1

公开(公告)日：2001-07-03

申请号：US08945625

申请日：1998-01-05

申请人： Ronald M. Teather ,  Robert J. Forster ,  Martin Kalmokoff

发明人： Ronald M. Teather ,  Robert J. Forster ,  Martin Kalmokoff

IPC分类号： C12P2102

CPC分类号： C12R1/01 ,  A61K38/00 ,  C07K14/195 ,  Y10S435/801 ,  Y10S530/82 ,  Y10S530/825

摘要： Proteinaceous antibiotics produced by ruminal bacteria are provided. The diverse group of ruminal bacteria known as Butyrivibrio spp. is a preferred source of such proteinaceous antibiotics. The proteinaceous antibiotics are generally resistant to gastric proteases, exhibit a high level of hydrophobicity, are effective to inhibit growth of target organisms under anaerobic conditions, are ineffective in aerobic conditions, and have a molecular weight of less than about 5 kDa. Also provided are methods for identifying ruminal bacteria which produce such proteinaceous antibiotics, and methods for producing the proteinaceous antibiotics.

摘要翻译： 提供了由瘤胃细菌产生的蛋白质抗生素。 被称为Butyrivibrio spp。的不同组的瘤胃细菌 是这种蛋白质抗生素的优选来源。 蛋白质抗生素通常对胃蛋白酶具有抗性，表现出高水平的疏水性，在厌氧条件下有效抑制靶生物的生长，在需氧条件下无效，分子量小于约5kDa。 还提供了用于鉴定产生这种蛋白质抗生素的瘤胃细菌的方法，以及用于生产蛋白质抗生素的方法。

公开(公告)号：US5763170A

公开(公告)日：1998-06-09

申请号：US463890

申请日：1995-06-05

申请人： Margaret Raybuck

发明人： Margaret Raybuck

IPC分类号： C12M1/34 ,  G01N15/14 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  C07K13/00

CPC分类号： G01N15/14 ,  G01N2015/149 ,  Y10S435/801

摘要： A method for forming and using an array of, e.g., bacteria, yeast or bacteriophage for the purpose of identifying particular constituents thereof. The array is formed by directing a stream of droplets, each containing on average about 1 or a few biological particles, at spaced locations in an array on a surface, e.g., a nylon membrane or agar gel.

摘要翻译： 用于形成和使用例如细菌，酵母或噬菌体的阵列的方法，用于鉴定其特定成分。 该阵列通过在表面上例如尼龙膜或琼脂凝胶上的阵列中的间隔位置处引导每个平均含有约1个或几个生物颗粒的液滴流而形成。

公开(公告)号：US5188946A

公开(公告)日：1993-02-23

申请号：US860684

申请日：1992-03-30

申请人： N. Robert Ward, Jr. ,  Philip J. Lozier

发明人： N. Robert Ward, Jr. ,  Philip J. Lozier

IPC分类号： C12M1/24

CPC分类号： C12M23/08 ,  C12M29/20 ,  Y10S435/801

摘要： There is disclosed an apparatus, a device, and a method using the inventive device and apparatus for the sequential enrichment of a particular microorganism to a threshold level to allow for its detection. The use of the apparatus for sequential microbial enrichment shortens the time period normally spent to enrich samples to permit the growth of enough microorganisms to detect the particular microorganism(s) of interest. The invention eliminates the need for multiple manual transfers of potentially biohazardous materials.

摘要翻译： 公开了一种使用本发明的装置和装置的装置，装置和方法，用于将特定微生物顺序富集至阈值水平以允许其检测。 用于连续微生物富集的装置的使用缩短了通常用于富集样品以允许生长足够的微生物以检测感兴趣的特定微生物的时间段。 本发明消除了对潜在的生物危害材料的多次手动转移的需要。

公开(公告)号：US5147801A

公开(公告)日：1992-09-15

申请号：US452767

申请日：1989-12-19

申请人： Akira Suzuki ,  Yuji Sakamoto ,  Yuko Masuda

发明人： Akira Suzuki ,  Yuji Sakamoto ,  Yuko Masuda

IPC分类号： C12N1/00 ,  C12M1/16

CPC分类号： C12M25/14 ,  C12M23/04 ,  Y10S435/801 ,  Y10S435/805 ,  Y10T428/1307 ,  Y10T428/31902 ,  Y10T428/31993

摘要： A culture medium device in the form of a sheet comprises a base sheet member composed of an upper sheet having a hydrophilic property such as filter paper, a lower sheet having a water repellent property covering a lower surface of the upper hydrophilic sheet and a sheet member having a water repellent property preferably in the form of a film having a character suitable for bacteria to be treated. A gel agent or gelatinizer containing bacteria culturing nutrient is dispersed on an upper surface of the upper hydrophilic sheet of the base sheet member and the gel agent or gelatinizer is absorbed in the upper hydrophilic sheet and then solidified. The upper water repellent sheet member is applied so as to cover the upper surface of the upper hydrophilic sheet of the base sheet member.

公开(公告)号：US5034331A

公开(公告)日：1991-07-23

申请号：US763530

申请日：1985-08-08

申请人： John H. Brewer

发明人： John H. Brewer

IPC分类号： C12M1/04

CPC分类号： C12M23/10 ,  C12M37/04 ,  Y10S435/801

摘要： Metal hydrosulfites are found to be advantageous reducing agents for removing oxygen from contained atmospheres. When dissolved in alkaline aqueous gels, the hydrosulfites are effective to initiate and maintain anaerobiosis for culturing oxygen-sensitive microorganisms and animal tissues.

摘要翻译： 发现金属连二亚硫酸盐是用于从含有气氛中除去氧气的有利的还原剂。 当溶解在碱性水凝胶中时，连二亚硫酸盐有效引发和维持厌氧菌，用于培养氧敏感微生物和动物组织。

公开(公告)号：US4929557A

公开(公告)日：1990-05-29

申请号：US176344

申请日：1988-03-31

申请人： Garabed Antranikian ,  Gerhard Gottschalk

发明人： Garabed Antranikian ,  Gerhard Gottschalk

IPC分类号： C12N9/26 ,  C12N9/28 ,  C12N9/44 ,  C12R1/01

CPC分类号： C12N9/2457 ,  C12N9/2411 ,  C12N9/2417 ,  C12Y302/01041 ,  Y10S435/801 ,  Y10S435/822

摘要： At least one type of excretable, thermostable amylolytic enzyme, such as amylase and pullulanase, can be recovered from a culture medium in which thermophilic anaerobic bacteria, such as Thermoanaerobacter finii or Thermobacteroides acetoethylicus, are propagated anaerobically in continuous culture, in the presence of a higher saccharide as carbon source, such an enzyme having a high stability at temperatures between 60.degree. and 70.degree. C. in absence of metal ions and substrate and under aerobic conditions.

摘要翻译： 可以从培养基中回收至少一种可排除的热稳定淀粉分解酶，例如嗜热厌氧细菌，例如热嗜热杆菌（Thermanaerobacter finii）或嗜热醋杆菌（Thermobacteroides acetoethylicus），在连续培养物中厌氧地繁殖，在 较高的糖类作为碳源，这种酶在不存在金属离子和底物的条件下，在有氧条件下，在60〜70℃的温度下具有高稳定性。

公开(公告)号：US4800157A

公开(公告)日：1989-01-24

申请号：US773916

申请日：1985-09-09

申请人： Tom E. Eaton ,  Lynda M. Ford ,  Otis W. Godfrey, Jr. ,  Mary L. B. Huber ,  Milton J. Zmijewski, Jr.

发明人： Tom E. Eaton ,  Lynda M. Ford ,  Otis W. Godfrey, Jr. ,  Mary L. B. Huber ,  Milton J. Zmijewski, Jr.

IPC分类号： C07K7/08 ,  C12P21/04 ,  C12N1/20 ,  C12R1/465

CPC分类号： C12R1/465 ,  C07K7/08 ,  Y10S435/801 ,  Y10S435/886

摘要： Improved fermentation process for producing the gram-positive A-21978C antibiotics which comprises cultivating a new strain of Streptomyces roseosporus, NRRL 15998, and the biologically purified culture of this microorganism are provided.

摘要翻译： 提供了用于生产革兰氏阳性A-21978C抗生素的改进的发酵方法，其包括培养新的玫瑰孢链霉菌菌株NRRL 15998和该微生物的生物纯化培养物。