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公开(公告)号：US5354655A
公开(公告)日：1994-10-11
申请号：US174848
申请日：1988-03-29
IPC分类号： G01N33/543 , C12M1/34 , C12Q1/00 , C12Q1/04 , C12Q1/18 , C12Q1/68 , G01N30/88 , G01N33/53 , G01N33/538
CPC分类号： C12M41/36 , C12M21/18 , C12Q1/00 , C12Q1/04 , C12Q1/18 , C12Q2334/22 , Y10S435/803 , Y10S435/962 , Y10S436/824
摘要： There is disclosed a process and a device for detecting and measuring (1) the amount of enzyme present as a detecting system following a nucleic acid hybridization reaction or immunoreaction; (2) the level and activity of free enzyme in a biological sample; (3) the level of enzyme from contaminating microorganisms present in a sample; and (4) enzymes from pure culture isolates for microbial identification and antimicrobial susceptibility testing.
摘要翻译： 公开了用于检测和测量（1）作为核酸杂交反应或免疫反应后的检测系统存在的酶的量的方法和装置; （2）生物样品中游离酶的水平和活性; （3）样品中存在的污染微生物的酶水平; 和（4）来自纯培养分离物的酶用于微生物鉴定和抗微生物药敏试验。
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2.发明授权
公开(公告)号：US5188946A
公开(公告)日：1993-02-23
申请号：US860684
申请日：1992-03-30
IPC分类号： C12M1/24
CPC分类号： C12M23/08 , C12M29/20 , Y10S435/801
摘要： There is disclosed an apparatus, a device, and a method using the inventive device and apparatus for the sequential enrichment of a particular microorganism to a threshold level to allow for its detection. The use of the apparatus for sequential microbial enrichment shortens the time period normally spent to enrich samples to permit the growth of enough microorganisms to detect the particular microorganism(s) of interest. The invention eliminates the need for multiple manual transfers of potentially biohazardous materials.
摘要翻译： 公开了一种使用本发明的装置和装置的装置，装置和方法，用于将特定微生物顺序富集至阈值水平以允许其检测。 用于连续微生物富集的装置的使用缩短了通常用于富集样品以允许生长足够的微生物以检测感兴趣的特定微生物的时间段。 本发明消除了对潜在的生物危害材料的多次手动转移的需要。
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公开(公告)号：US5100801A
公开(公告)日：1992-03-31
申请号：US302009
申请日：1989-01-26
IPC分类号： C12M1/24
CPC分类号： C12M23/08 , C12M29/20 , Y10S435/80 , Y10S435/813 , Y10S435/819
摘要： There is disclosed an apparatus, a device, and a method using the inventive device and apparatus for the sequential enrichment of a particular microorganism to a threshold level to allow for its detection. The use of the apparatus for sequential microbial enrichment shortens the time period normally spent to enrich samples to permit the growth of enough microorganisms to detect the particular microorganism(s) of interest. The invention eliminates the need for multiple manual transfers of potentially biohazardous materials.
摘要翻译： 公开了一种使用本发明的装置和装置的装置，装置和方法，用于将特定微生物顺序富集至阈值水平以允许其检测。 用于连续微生物富集的装置的使用缩短了通常用于富集样品以允许生长足够的微生物以检测感兴趣的特定微生物的时间段。 本发明消除了对潜在的生物危害材料的多次手动转移的需要。
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公开(公告)号：US4920063A
公开(公告)日：1990-04-24
申请号：US773201
申请日：1985-09-09
申请人： N. Robert Ward, Jr.
发明人： N. Robert Ward, Jr.
IPC分类号： C12M1/24 , C12M1/34 , C12N1/00 , C12Q1/02 , C12Q1/04 , C12Q1/10 , C12R1/42 , G01N33/02 , G01N33/12 , G01N33/569
CPC分类号： C12Q1/10 , C12M41/36 , C12Q1/02 , G01N33/02 , G01N33/569 , G01N33/56916 , G01N2333/255 , G01N33/12 , Y10S435/879
摘要： Methods and associated devices for detecting the presence of a particular motile organism within a sample are disclosed. The sample may be derived from dry milk, raw meat, poultry or a clinical specimen. A preferred method includes inoculating a selective enrichment medium containing a chemotactic attractant with the sample and contacting the selective enrichment medium with a nonselective motility medium containing a chemotatic attractant in a concentration less than the attractant concentration in the selective enrichment medium. Upon incubation, the motile organism metabolizes the chemotactic attractant, allowing the organism to move into the motility medium where it interacts with antibodies specific for the organism, thereby causing the formation of a persistent immobilization band. The methods are particularly useful in detecting Salmonella.
摘要翻译： 公开了用于检测样品中特定运动生物体的存在的方法和相关装置。 样品可以来自干乳，生肉，家禽或临床标本。 优选的方法包括将含有趋化性引诱剂的选择性富集培养基与样品接触，并使选择性富集培养基与含有浓度小于选择性富集培养基中引诱剂浓度的趋化性引诱剂的非选择性运动培养基接触。 在孵育后，运动生物体代谢趋化性引诱剂，允许生物进入动力培养基，其中与生物体特异的抗体相互作用，从而形成持续的固定带。 该方法特别适用于检测沙门氏菌。
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公开(公告)号：USD303711S
公开(公告)日：1989-09-26
申请号：US10314
申请日：1987-02-03
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公开(公告)号：US06585413B1
公开(公告)日：2003-07-01
申请号：US09978481
申请日：2001-10-16
IPC分类号： B65D3330
CPC分类号： B65D33/30 , B31B70/81 , B65D33/007 , B65D75/5805 , Y10S383/905
摘要： The body of a sterile collection bag has opposed sidewalls and an upper body end. The interior of the body defines a sterile collection space for an object. First and second flexible closure strips having integrated centrally located wires are attached to the bag sidewalls and extend beyond the side wall edges. The upper end of the body includes one or more small notches cut into the body sidewalls to facilitate tearing of the upper body end from the bag body, with abrupt edges of the strips acting as tear guides. Pull tabs attached to the closure strips can be drawn apart to open the mouth of the bag for insertion of a sample.
摘要翻译： 无菌收集袋的身体具有相对的侧壁和上身端。 身体的内部为物体定义了无菌收集空间。 具有集成的位于中心的导线的第一和第二柔性封闭带附接到袋侧壁并延伸超过侧壁边缘。 主体的上端包括切入主体侧壁的一个或多个小切口，以便于使上身端部从袋体撕裂，带的突出边缘用作泪液引导件。 附接到封闭条上的拉片可以被分开以打开袋的口以插入样品。
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公开(公告)号：US5403721A
公开(公告)日：1995-04-04
申请号：US51569
申请日：1993-04-21
申请人： N. Robert Ward, Jr. , John P. DesRosier , Elliott D. Marshall, III , Judith Ford , Nancy J. S. Mallinak
发明人： N. Robert Ward, Jr. , John P. DesRosier , Elliott D. Marshall, III , Judith Ford , Nancy J. S. Mallinak
CPC分类号： C12Q1/04 , C12Q1/34 , C12Q2334/52 , G01N2333/924 , Y10S435/805 , Y10S435/97
摘要： There is disclosed an improved method for testing for the presence of a particular microorganism or group of microorganisms characterized by a particular enzyme. The method uses a dye-forming substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate when cleaved by the enzyme. The precipitate is concentrated to the polymer matrix and/or solid support member to create a visible reaction product, wherein the amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming substrate throughout a polymer system or on the surface of a solid support member.
摘要翻译： 公开了一种用于测试以特定酶为特征的特定微生物或微生物组的存在的改进方法。 该方法在整个聚合物基质中或在固体支持构件的表面上使用染料形成底物，其在被酶切割时形成着色沉淀。 将沉淀物浓缩到聚合物基质和/或固体支持构件以产生可见的反应产物，其中所需的染料形成底物的量不依赖于样品的大小。 本发明还包括酶指示剂装置，其在整个聚合物系统中或在固体支持构件的表面上包含染料形成基质。
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公开(公告)号：US07238253B2
公开(公告)日：2007-07-03
申请号：US10847649
申请日：2004-05-17
IPC分类号： B65D33/30
CPC分类号： B65D33/30 , B31B70/8123 , B65D33/007 , B65D33/20 , Y10T156/1052 , Y10T156/1077 , Y10T156/108 , Y10T156/1097 , Y10T156/1343 , Y10T156/1741 , Y10T156/1768 , Y10T156/1778
摘要： A placement component rapidly moves individual bag blanks from a pack or magazine to a conveyor system in uniformly spaced relationship. Each bag blank has opposite surfaces. While the conveyor system continues to move the bags, opening tabs are automatically applied to opposite surfaces of each bag, followed by high speed application and cutting of sideways extending flexible closure strips. The bag blanks are moved continuously from the placement component to the high speed cutting section, as compared to incremental movement, for high speed manufacturing of specialized bags.
摘要翻译： 放置组件将各个袋坯料从包装或料仓快速移动到输送系统中，并以均匀间隔的关系。 每个袋坯都有相对的表面。 当输送机系统继续移动袋子时，打开的舌片被自动地施加到每个袋子的相对表面上，随后高速施加和切割侧向延伸柔性封闭条带。 与增量运动相比，袋坯料从放置部件连续地移动到高速切割部分，用于高速制造专门的袋子。
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公开(公告)号：US4563418A
公开(公告)日：1986-01-07
申请号：US621182
申请日：1984-06-15
申请人： N. Robert Ward, Jr.
发明人： N. Robert Ward, Jr.
CPC分类号： C12Q1/10 , C12Q1/02 , G01N33/569 , G01N33/56916 , G01N2333/255 , G01N33/12
摘要： A motility-immunoimmobilization method permits the detection of a particular motile organism, such as Salmonella, in a sample.
摘要翻译： 运动免疫免疫方法允许在样品中检测特定的运动生物体，例如沙门氏菌。
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公开(公告)号：US06273608B1
公开(公告)日：2001-08-14
申请号：US09608952
申请日：2000-06-30
IPC分类号： B65D3300
CPC分类号： B65D33/30
摘要： A sterile collection bag and method of opening using wire mechanisms. The bag includes a body and an opening mechanism. The body is formed of opposed side walls and an upper body end. The interior of the body defines a sterile collection space for an object. The opening mechanism includes first and second flexible metal strips each having a first end, a second end, and a mid-section. The mid-section of each metal strip is attached to the center section of a side wall. The metal strips are longer than the width of the body so as to extend beyond the side wall edges. The ends of the metal strips are unattached to one another. In one embodiment, the upper end of the body includes an opening. In another embodiment, the body includes a lateral tear line located below the body upper end, with the opening mechanism located below the tear line. During use, the ends of the metal strips are bent away from one another; the ends are pinched together and moved toward one another to cause the metal strips to bulge outward at their mid-section. This further causes the body side walls to separate so that the object may be inserted into the bag. When using a lateral tear line, the upper end of the body is first torn away along the lateral tear line.
摘要翻译： 无菌收集袋和使用线机构打开的方法。 袋子包括一个主体和一个打开机构。 身体由相对的侧壁和上身端形成。 身体的内部为物体定义了无菌收集空间。 打开机构包括第一和第二柔性金属带，每个具有第一端，第二端和中间部。 每个金属条的中间部分连接到侧壁的中心部分。 金属条比主体的宽度长，以便延伸超过侧壁边缘。 金属带的端部彼此不相连。 在一个实施例中，主体的上端包括开口。 在另一个实施例中，主体包括位于主体上端下方的侧向撕裂线，打开机构位于撕裂线下方。 在使用过程中，金属条的端部相互弯曲; 端部被夹在一起并相互移动以使金属带在其中间部分向外凸出。 这进一步导致身体侧壁分离，使得物体可以插入袋中。 当使用横向撕裂线时，主体的上端首先沿着侧撕裂线撕开。
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