公开(公告)号：US08815499B2

公开(公告)日：2014-08-26

申请号：US13517250

申请日：2010-12-17

申请人： Björn Alriksson ,  Leif Jönsson ,  Sune Wännström

发明人： Björn Alriksson ,  Leif Jönsson ,  Sune Wännström

IPC分类号： C12Q3/00 ,  C12P7/16 ,  C12N1/38 ,  C12P7/46 ,  C12P7/10

CPC分类号： C12P7/10 ,  C12N1/38 ,  C12P7/16 ,  C12P7/46 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y10S435/813

摘要： The present invention provides a method for decreasing the fermentation inhibition in a fermentation of cellulose-derived material in a fermentor, wherein fermentation inhibitory properties of the material subjected to fermentation is decreased by an addition of at least one reducing agent to the fermentor. Further, there is provided a method of increasing the fermentability of a fermentation process comprising the steps of measuring the fermentability of the fermentation process and if the fermentability is below a reference value, then adding at least one reducing agent to the fermentation process.

摘要翻译： 本发明提供一种用于降低发酵罐中纤维素衍生物质的发酵发酵抑制的方法，其中通过向发酵罐中加入至少一种还原剂来降低发酵物质的发酵抑制性能。 此外，提供了一种增加发酵方法的发酵能力的方法，包括以下步骤：测量发酵过程的发酵能力，如果发酵能力低于参考值，则在发酵过程中加入至少一种还原剂。

公开(公告)号：US08501460B2

公开(公告)日：2013-08-06

申请号：US13008553

申请日：2011-01-18

申请人： James Francis Furey

发明人： James Francis Furey

IPC分类号： C12M1/02

CPC分类号： C12M29/06 ,  C12M23/26 ,  C12M23/28 ,  C12M29/00 ,  C12M29/16 ,  C12M29/18 ,  C12M47/10 ,  Y10S435/813

摘要： The present invention is in the field of cell bioreactors, and specifically in the field of disposable bioreactors.

摘要翻译： 本发明涉及细胞生物反应器领域，特别是在一次性生物反应器领域。

公开(公告)号：US20110195055A1

公开(公告)日：2011-08-11

申请号：US12930471

申请日：2011-01-07

申请人： Joel S. Greenberger ,  Paul A. DiMilla ,  Michael M. Domach ,  Raymond K. Houck

发明人： Joel S. Greenberger ,  Paul A. DiMilla ,  Michael M. Domach ,  Raymond K. Houck

IPC分类号： A61K35/12 ,  C12M1/34 ,  C12M1/36 ,  C12N5/071 ,  C12Q1/02 ,  C12N5/09 ,  C12N5/0789 ,  A61P37/00 ,  A61P7/00

CPC分类号： G01N33/5008 ,  C12M23/12 ,  C12M41/36 ,  C12M41/46 ,  C12M41/48 ,  G02B21/16 ,  G02B21/34 ,  Y10S435/802 ,  Y10S435/808 ,  Y10S435/809 ,  Y10S435/813

摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.

摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且其中细胞可以在环境被动态控制并保持在所需条件下被检查。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。

公开(公告)号：US07521203B2

公开(公告)日：2009-04-21

申请号：US11123715

申请日：2005-05-06

申请人： WengLong Roy Lin ,  Firoz Rustom Mistry ,  Arun Narayanaswamy Tholudur ,  Edward Todd Sorensen ,  Wan-Seop Kim ,  Dana Perrin

发明人： WengLong Roy Lin ,  Firoz Rustom Mistry ,  Arun Narayanaswamy Tholudur ,  Edward Todd Sorensen ,  Wan-Seop Kim ,  Dana Perrin

IPC分类号： G06F19/00 ,  G01N33/48 ,  G01N33/50 ,  C12P1/00

CPC分类号： C12M41/48 ,  C12M41/26 ,  C12P1/00 ,  Y10S435/813 ,  Y10S435/818

摘要： Embodiments of the present invention generally relate to novel fed-batch fermentations wherein processes of DO-stat and pH-stat are combined for nutrient feeding control.

摘要翻译： 本发明的实施方案通常涉及新型补料分批发酵，其中将DO-stat和pH-stat的方法组合用于营养喂养控制。

公开(公告)号：US20050282257A1

公开(公告)日：2005-12-22

申请号：US11191310

申请日：2005-07-28

申请人： Valle Fernando ,  Noemi Mejia ,  Alan Berry

发明人： Valle Fernando ,  Noemi Mejia ,  Alan Berry

IPC分类号： C12N1/20 ,  C12N1/21 ,  C12N15/52 ,  C12P1/00 ,  C12P13/22 ,  C12P19/04

CPC分类号： C12P13/22 ,  C12N1/20 ,  C12N15/52 ,  C12P1/00 ,  Y10S435/813

摘要： This invention describes methods for enhancing carbon flow into a pathway of a host cell to enhance the biosynthetic production of compounds therefrom, the host cells being selected based on being phenotypically Pts−/glucose+. Such host cells are capable of transporting glucose without consuming PEP, resulting in conservation of PEP which can be re-directed into the pathway in order to enhance the production of desired compounds along the pathway. Pts−/glucose+ mutants have been shown to be advantageous for the enhanced production of the aromatic amino acids.

公开(公告)号：US06686194B1

公开(公告)日：2004-02-03

申请号：US09857226

申请日：2001-06-01

申请人： Rupert Mutzel ,  Philippe Marliere

发明人： Rupert Mutzel ,  Philippe Marliere

IPC分类号： C12M136

CPC分类号： C12M23/58 ,  C12M37/00 ,  Y10S435/813

摘要： The present invention relates to a method and a device for selecting accelerated proliferation of living cells in suspension. The culture apparatus (2) of the present invention enables cells to proliferate in suspension over unlimited periods of time. Natural selection results in the accumulation of genetic variants which are increasingly better adapted to the chosen culture conditions. The organisms used can be prokaryotic or eukaryotic. The organisms used can be naturally occurring organisms or genetically modified organisms. The culture apparatus of the present invention is also suitable for using continuous, periodical or conditional culture conditions. The physical and chemical characteristics of the culture media used can be chosen by the user. The requirement that a population of cells proliferates exclusively in suspension in continuous culture conditions is satisfied by the periodical transfer of the organism suspension from a first culture vessel into a second culture vessel. After the transfer, the first culture vessel is subjected to a sterilizing treatment and the sterilizing agent is optionally neutralized, so that the first culture vessel is ready for the culture to be transferred back from the second culture vessel. The second culture vessel is then sterilized and neutralized.

摘要翻译： 本发明涉及用于选择悬浮液中活细胞加速增殖的方法和装置。 本发明的培养装置（2）能够使细胞在无限期内悬浮增殖。 自然选择导致遗传变异体的积累，其越来越适应于所选择的培养条件。 所用的生物体可以是原核生物或真核生物。 所使用的生物体可以是天然存在的生物体或转基因生物。 本发明的培养装置也适用于连续，周期或条件培养条件。 使用的培养基的物理和化学特性可由用户选择。 通过将生物体悬浮液从第一培养容器定期转移到第二培养容器中，满足在连续培养条件下悬浮的细胞群体增殖的要求。 转移后，对第一培养容器进行灭菌处理，任选地中和杀菌剂，使第一培养容器准备从第二培养容器中转移回培养物。 然后将第二培养容器消毒并中和。

公开(公告)号：US06620614B1

公开(公告)日：2003-09-16

申请号：US09674372

申请日：2000-12-04

申请人： Peter Lüth ,  Ute Eiben

发明人： Peter Lüth ,  Ute Eiben

IPC分类号： C12C115

CPC分类号： C12M41/24 ,  C12M21/16 ,  C12M23/24 ,  C12M23/34 ,  Y10S435/813 ,  Y10S435/819

摘要： The invention concerns a solid state fermenter in particular for large volumes as well as a procedure for solid state fermentation. The task of the present invention was the development of a solid state fermenter for large volumes and of a procedure for solid state fermentation, that allows an economic application of the solid state fermentation for little competitive micro-organisms in large fermenters. The solid state fermenter according to the invention is characterized by representing a module fermenter, where at least two module bases, that are permeable for air and water, are arranged on top of each other, which are connected with the wall of the vessel in such a manner, that neither air nor water can pass laterally, the existence of a cultivation substrate for the micro-organisms, which have to be cultivated, on the module bases, a cooling device below every module base and the fermenter being closed with a lid.

摘要翻译： 本发明涉及特别是大体积的固态发酵罐以及固态发酵方法。本发明的任务是开发大容量的固态发酵罐和用于固态发酵的方法，其允许 固体发酵对大型发酵罐中的少量竞争性微生物的经济应用。根据本发明的固态发酵罐的特征在于代表模块发酵罐，其中至少两个可渗透空气和水的模块基底是 排列在彼此之上，以这样的方式与容器的壁连接，使得空气和水不能横向通过，必须培养用于微生物的栽培基质的存在 模块底座，每个模块底部下方的冷却装置和用盖子关闭的发酵罐。

公开(公告)号：US20020155487A1

公开(公告)日：2002-10-24

申请号：US10114892

申请日：2002-04-02

发明人： Joel S. Greenberger ,  Paul A. DiMilla ,  Michael M. Domach ,  Raymond K. Houck

IPC分类号： C12Q001/68 ,  C12M001/34

CPC分类号： G01N33/5008 ,  C12M23/12 ,  C12M41/36 ,  C12M41/46 ,  C12M41/48 ,  G02B21/16 ,  G02B21/34 ,  Y10S435/802 ,  Y10S435/808 ,  Y10S435/809 ,  Y10S435/813

摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.

摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且其中细胞可以在环境被动态控制并保持在所需条件下被检查。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。

公开(公告)号：US20010051371A1

公开(公告)日：2001-12-13

申请号：US09227792

申请日：1999-01-08

发明人： DALE VILMER KIPLINGER ,  JUDITH GAYLE PRUITT ,  JOSE EDURADO EVARO ,  ROBERT CLARENCE PEARCE III

IPC分类号： C07F001/00 ,  C07K001/00 ,  C07C001/00 ,  C07H001/00 ,  C07J001/00

CPC分类号： C12M27/22 ,  C12M23/02 ,  C12M29/02 ,  C12M29/14 ,  C12M29/18 ,  C12M41/02 ,  C12M41/40 ,  Y10S435/813 ,  Y10S435/818

摘要： An automated system and method for cultivating bacteria in a fluid medium and thereafter selectively discharging the fluid medium, wherein an initial supply of the selected strain or strains of bacteria is combined with nutrients and water in a biogenerator in the presence of air to promote mixing and bacterial cultivation. The disclosed system and method utilize a vortex created by recirculation of the fluid medium to achieve aeration and mixing without substantial foaming. The disclosed system and method are particularly useful for supplying bacteria to control grease accumulation in restaurant grease traps.

摘要翻译： 一种用于在流体培养基中培养细菌并随后选择性排出流体培养基的自动化系统和方法，其中所选择的菌株或菌株的初始供应在生物发生器中与营养物质和水组合以促进混合， 细菌培养。 所公开的系统和方法利用由流体介质的再循环产生的涡流以实现通气和混合而没有实质的发泡。 所公开的系统和方法特别适用于供应细菌以控制餐厅油脂捕集器中的油脂积聚。

公开(公告)号：US06225089B1

公开(公告)日：2001-05-01

申请号：US09333208

申请日：1999-06-15

申请人： Kevin Keqin Chen

发明人： Kevin Keqin Chen

IPC分类号： C12P2106

CPC分类号： C07K14/21 ,  C12P7/42 ,  Y10S435/803 ,  Y10S435/813 ,  Y10S435/827 ,  Y10S435/877 ,  Y10S435/911 ,  Y10T436/143333

摘要： This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.

摘要翻译： 本发明涉及从门氏假单胞菌（Pseudomonas mendocina）中分离出一种新的推定流出基因。 推定的流出基因可用于探测生物体的外排系统，以了解溶剂耐受机制。 本发明还提供了缺乏该基因的假单胞菌门氏菌属菌株。 该菌株在氯霉素存在下不能生长，并且与野生型菌株相比，在高浓度PHBA存在下生长缓慢。