公开(公告)号：US3964971A

公开(公告)日：1976-06-22

申请号：US517797

申请日：1974-10-24

Applicant: Bela Johan ,  Laszlo Szemler ,  Janos Fulop ,  Tamas Szontagh ,  Emilia Simonovits nee Czink ,  Judit Bekes nee Erdos ,  Laszlo Kuti ,  Robert Toros ,  Denes Szekely ,  Laszlo Szabo ,  Karoly Seitz ,  Tade Vajda ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargital nee Franyo ,  Karoly Polinszky

Inventor： Bela Johan ,  Laszlo Szemler ,  Janos Fulop ,  Tamas Szontagh ,  Emilia Simonovits nee Czink ,  Judit Bekes nee Erdos ,  Laszlo Kuti ,  Robert Toros ,  Denes Szekely ,  Laszlo Szabo ,  Karoly Seitz ,  Tade Vajda ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargital nee Franyo ,  Karoly Polinszky

IPC: C07H23/00 ,  C12P5/02 ,  C12P19/42 ,  C12D5/06

CPC classification number: C12P19/42 ,  C12P5/023 ,  Y02E50/343 ,  Y10S435/801 ,  Y10S435/813

Abstract: The invention relates to a method for increasing the vitamin B.sub.12 production of fermentation processes performed in a known way with a mixed population of mesophilic methane-producing bacteria under anaerobic conditions. According to the invention in an enrichment period of preferably 4 to 7 days a nutrient concentrate containing mainly inorganic ammonium compounds as nitrogen source and mainly methanol as carbon source and having a N:C weight ratio of 1:10 to 1:20, preferably 1:11 to 1:15 is added in daily portions to a fermentation broth obtained from a usual vitamin B.sub.12 fermentation process and containing living bacterium populations so as to increase the total concentration of assimilable nitrogen by a factor of maximum 4 until the end of the enrichment period, and thereafter the fermentation is terminated and the obtained fermentation broth with an increased vitamin B.sub.12 content is processed, or the fermentation is continued by periodically removing a portion of the fermentation broth and supplementing it with a same volume of fresh nutrient medium containing mainly inorganic ammonium compounds as nitrogen source and mainly methanol as carbon source and having a N:C weight ratio of 1:10 to 1:20, preferably 1:11 to 1:15.By this process fermentation broths with as high vitamin B.sub.12 concentration as 40.000 mcg./l. can be obtained.

Abstract translation: 本发明涉及一种增加在厌氧条件下用嗜温甲烷生产菌混合种群以已知方法进行的发酵过程的维生素B12产生的方法。 根据本发明，优选4至7天的富集期间，主要以无机铵化合物为氮源，主要为甲醇为碳源，且N:C重量比为1:10至1:20，优选1 ：将11〜1:15加入到通常的维生素B12发酵方法得到的发酵液中，并含有活细菌种群，以将可吸收氮的总浓度提高到4倍直至浓缩结束为止 期间，然后终止发酵，并且处理具有增加的维生素B12含量的所得发酵液，或者通过周期性地除去一部分发酵液并补充相同体积的主要含有无机物的新鲜营养培养基来继续发酵 铵化合物作为氮源，主要是甲醇作为碳源，N:C重量比为1:10至1:20， 优选1:11至1:15。

公开(公告)号：US3979259A

公开(公告)日：1976-09-07

申请号：US517788

申请日：1974-10-24

Applicant: Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

Inventor： Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

IPC: C12N1/32 ,  C12P5/02 ,  C12P19/42 ,  C12R1/01 ,  C12D5/06

CPC classification number: C12P5/023 ,  C12P19/42 ,  Y02E50/343 ,  Y10S435/801 ,  Y10S435/802 ,  Y10S435/813

Abstract: The invention relates to a process for the production of a fermentation broth with increased vitamin B.sub.12 content by a fermentation process carried out with a methane-producing mixed bacterium population under anaerobic septic conditions in the presence of known nutrient components and precursors. According to the invention, methanol, preferably in an amount of 0.5 v/v %, is added for some days, preferably for 3 days to the fermentation broth containing vitamin B.sub.12 used as the starting substance. Thereafter a minor part, preferably 10% of the fermentation broth, is removed and an equal volume of a nutrient broth containing the usual components in tenfold concentrations is added periodically in every 5th to 12th day, then the removal of fermentation broth is interrupted for 0 to 2 days, preferably for 1 day, and only 0.4 to 1.5 v/v% of methanol are added to the fermentation broth, and subsequently a minor part, preferably 10% of the fermentation broth, is removed daily and an equal volume of a nutrient broth containing the usual components in the usual concentrations is added together with 0.4 to 1.5 v/v % of methanol, depending on the biogas production of the fermentation broth. This series of operations is repeated periodically during the complete fermentation procedure taking into consideration the pH of the fermentation broth.

Abstract translation: 本发明涉及通过在已知营养成分和前体的存在下在厌氧脓毒症条件下用产甲烷混合菌群进行的发酵方法来生产具有增加的维生素B12含量的发酵液的方法。 根据本发明，优选以0.5v / v％的量将甲醇加入含有用作起始物质的维生素B12的发酵液中几天，优选3天。 此后，除去少量的发酵肉汤，并且每5至12天定期添加等体积的含有十倍浓度的通常组分的营养肉汤，然后将发酵肉汤的去除中断0 至2天，优选1天，并且仅将0.4至1.5v / v％的甲醇加入到发酵液中，随后每天除去少部分，优选10％的发酵液，并加入等体积的 根据发酵液的生物气产量，含有通常浓度的通常组分的营养肉汤与0.4至1.5v / v％的甲醇一起加入。 考虑到发酵液的pH值，在整个发酵过程中定期重复这一系列操作。