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    Means for biologically controlling cryptogamic plant diseases
    1.
    发明申请
    Means for biologically controlling cryptogamic plant diseases 失效
    用于生物控制密码植物病害的手段

    公开(公告)号：US20050271629A1

    公开(公告)日：2005-12-08

    申请号：US10520294

    申请日：2003-07-01

    申请人： Bernard Paul

    发明人： Bernard Paul

    IPC分类号： A01N63/04 ,  A01N63/00

    CPC分类号： A01N63/04 ,  Y10S435/837 ,  Y10S435/838 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/88 ,  Y10S435/938 ,  Y10S435/945 ,  A01N63/00 ,  A01N2300/00

    摘要： The invention concerns the application of compositions of micro-organisms in biological control of vin crptogamic diseases. Said composition comprises a mixture of at least one bacterium and at least one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant. The invention also concerns bacterial and yeast strains, as well as biofungicide formulations containing an efficient amount of at least one composition of micro-organisms including in mixture at least one bacterium and one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant, and a composition of filamentous fungi, in particular of the genus Pichia, Pythium, Trichoderma, Gliocladium, Ampelomyces, Talaromyces, Epicococcum, combined with an inert carrier. The invention is useful for treating cryptogamic plant diseases, in particular crop plants and vine.

    摘要翻译： 本发明涉及微生物组合物在葡萄球菌病的生物学控制中的应用。 所述组合物包含至少一种细菌和至少一种酵母，所述细菌或细菌和对植物无毒的酵母的混合物。 本发明还涉及细菌和酵母菌株，以及含有有效量的至少一种微生物组合物的生物杀真菌剂，包括至少一种细菌和一种酵母混合物，细菌或细菌和酵母不是 对于植物是有毒的，以及丝状真菌的组合物，特别是毕赤酵母属，腐霉属，木霉属，胶霉属，Ampelomyces，Talaromyces，Epicococcum，与惰性载体结合的组合物。 本发明可用于治疗隐窝性植物病害，特别是作物植物和葡萄藤。

    Antimicrobial agent against acid-resistant and heat-resistant bacteria
    2.
    发明授权
    Antimicrobial agent against acid-resistant and heat-resistant bacteria 失效
    耐酸，耐热细菌的抗微生物剂

    公开(公告)号：US06329011B1

    公开(公告)日：2001-12-11

    申请号：US09680403

    申请日：2000-10-05

    申请人： Shigeru Oita

    发明人： Shigeru Oita

    IPC分类号： A61K3800

    CPC分类号： C07K14/415 ,  A23L2/02 ,  A23L2/44 ,  A61K38/168 ,  Y10S435/832 ,  Y10S435/833 ,  Y10S435/834 ,  Y10S435/835 ,  Y10S435/836 ,  Y10S435/837 ,  Y10S435/838 ,  Y10S435/839

    摘要： An antimicrobial agent with a high degree of safety is provided, which is derived from a natural product and can exhibit growth-inhibitory activity against acid-resistant and heat-resistant bacteria such as Alicyclobacillus acidoterrestris, which is resistant against pasteurization and causes spoilage of fruit juice. The antimicrobial agent against acid-resistant and heat-resistant bacteria contains as an effective ingredient alpha-type thionin and/or beta-type thionin. A preservative for fruit juice is also provided, which contains as an effective ingredient the alpha-type thionin and/or beta-type thionin.

    摘要翻译： 提供了具有高度安全性的抗微生物剂，其来自天然产物，并且可以对抗酸碱环境酸杆菌的抗酸耐热细菌表现出抑制生物的活性，其耐巴氏灭菌并引起水果的腐败 果汁。 针对耐酸耐热细菌的抗微生物剂含有α型硫素和/或β型硫素的有效成分。 还提供了果汁防腐剂，其含有α型硫素和/或β型硫素作为有效成分。

    .beta.-fructofuranosidase, its preparation and uses
    3.
    发明授权
    .beta.-fructofuranosidase, its preparation and uses 失效
    β-呋喃果糖苷酶，其制备和用途

    公开(公告)号：US5952204A

    公开(公告)日：1999-09-14

    申请号：US941551

    申请日：1997-09-30

    申请人： Tetsuya Nakada ,  Hiroto Chaen ,  Toshiyuki Sugimoto

    发明人： Tetsuya Nakada ,  Hiroto Chaen ,  Toshiyuki Sugimoto

    IPC分类号： C12N9/24 ,  C12P19/14 ,  C07H3/04 ,  C12P19/20

    CPC分类号： C12Y302/01026 ,  C12N9/2431 ,  Y10S435/832 ,  Y10S435/837

    摘要： A .beta.-fructofuranosidase with a molecular weight of 49,000.+-.5,000 daltons on SDS-PAGE, an isoelectric point of 4.6.+-.0.5, an optimum pH of about 5.5-6.0, and an optimum temperature of about 50.degree. C. in the presence of calcium ion. The enzyme acts on saccharides with a .beta.-fructofuranosidic linkage and other substances including other saccharides, sugar alcohols, and alcohols to produce fructosyl-transferred saccharides in a relatively high yield.

    摘要翻译： 在SDS-PAGE上的分子量为49,000 +/- 5,000道尔顿的β-呋喃果糖苷酶，等电点为4.6 +/- 0.5，最适pH为约5.5-6.0，最适温度为约50℃ 钙离子的存在。 该酶作用于具有β-呋喃果糖苷键的糖类和其它物质，包括其它糖类，糖醇和醇类，以相当高的产率产生果糖基转移的糖类。

    Fluorescent gram stain
    4.
    发明授权
    Fluorescent gram stain 失效
    荧光革染色

    公开(公告)号：US4665024A

    公开(公告)日：1987-05-12

    申请号：US656627

    申请日：1984-10-01

    申请人： James D. Mansour

    发明人： James D. Mansour

    IPC分类号： C12Q1/04 ,  C12Q1/14 ,  C12Q1/02 ,  G01N21/64

    CPC分类号： C12Q1/14 ,  C12Q1/04 ,  Y10S435/802 ,  Y10S435/808 ,  Y10S435/837 ,  Y10S435/849 ,  Y10S435/852 ,  Y10S435/857 ,  Y10S435/882 ,  Y10S435/883 ,  Y10S435/885 ,  Y10S436/80

    摘要： A method to determine the Gram sign of microorganisms includes staining the microorganisms with a plurality of fluorescent dyes, applying excitation energy to the stained microorganisms, and observing the color of the fluorescence emission of the stained microorganisms. Gram-positive and Gram-negative microorganisms stain different colors, and assignment of the Gram sign may be made on the basis of the color of the stained microorganisms.

    摘要翻译： 确定微生物的革兰氏号的方法包括用多种荧光染料染色微生物，向染色的微生物施加激发能，并观察染色的微生物的荧光发射的颜色。 革兰氏阳性和革兰氏阴性微生物染色不同的颜色，并且可以基于染色的微生物的颜色进行赋形。

    Assay method for amylase activity and method of producing maltose dehydrogenase for use therein
    5.
    发明授权
    Assay method for amylase activity and method of producing maltose dehydrogenase for use therein 失效
    淀粉酶活性的测定方法和生产用于其中的麦芽糖脱氢酶的方法

    公开(公告)号：US4427771A

    公开(公告)日：1984-01-24

    申请号：US311263

    申请日：1981-10-14

    申请人： Hideo Misaki ,  Eiji Muramatsu ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    发明人： Hideo Misaki ,  Eiji Muramatsu ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    IPC分类号： C12N9/04 ,  C12Q1/40 ,  C12N1/20 ,  C12Q1/32 ,  C12R1/11

    CPC分类号： C12N9/0006 ,  C12Q1/40 ,  G01N2400/18 ,  Y10S435/837

    摘要： An assay method for amylase activity in a biological specimen such as serum, saliva or urine. The enzyme amylase in the specimen is used to decompose a substrate which is a glucose polymer having a modified reducing terminal glucose residue or a cyclic glucose polymer. A component of the decomposed substrate is measured as an indication of amylase activity in the specimen. The residue may be amylose, amylopectin, starch, starch hydrolyzate, an etherified reducing terminal, an esterified reducing terminal, gluconolactone or a gluconic acid residue or its derivative. Decomposed substrate assay may be effected by contacting the same with maltose dehydrogenase and NAD or NADP, whereupon the assay is performed by measuring the amount of reduced NAD or reduced NADP, by reacting the same with reduced-form hydrogen transport colorimetric reaction reagent. This reagent may be a tetrazolium salt and diaphorase, or tetrazolium salt and phenazinemethosulfate. To remove pre-existing glucose and maltose present in the specimen, the specimen may be pretreated with alpha-glucosidase or kinase in the presence of Mg.sup.++ and ATP, the kinase being for example hexokinase. The preferred maltose dehydrogenase is produced by culturing Bacillus megaterium B-0779 FERM-P No. 5662.

    摘要翻译： 生物样品如血清，唾液或尿液中淀粉酶活性的测定方法。 试样中的酶淀粉酶用于分解具有修饰的还原性末端葡萄糖残基或环状葡萄糖聚合物的葡萄糖聚合物的底物。 测量分解的底物的组分作为样品中淀粉酶活性的指示。 残基可以是直链淀粉，支链淀粉，淀粉，淀粉水解物，醚化还原末端，酯化还原末端，葡萄糖酸内酯或葡萄糖酸残基或其衍生物。 分解的底物测定可以通过使其与麦芽糖脱氢酶和NAD或NADP接触来实现，因此通过使其与还原形式的氢转运比色反应试剂反应来测量还原的NAD或还原的NADP的量来进行测定。 该试剂可以是四唑盐和心律黄素，或四唑鎓盐和吩嗪甲硫酸盐。 为了除去样品中存在的现有葡萄糖和麦芽糖，可以在Mg ++和ATP存在下用α-葡糖苷酶或激酶预处理样品，所述激酶例如己糖激酶。 优选的麦芽糖脱氢酶是通过培养巨大芽孢杆菌B-0779 FERM-P No.5662产生的。

    Process for producing 7-amino desacetoxy cephalosporanic acid
    9.
    发明授权
    Process for producing 7-amino desacetoxy cephalosporanic acid 失效
    生产7-氨基甲酸羟丙酸的方法

    公开(公告)号：US3821081A

    公开(公告)日：1974-06-28

    申请号：US23508572

    申请日：1972-03-15

    申请人： TOYO JOZO KK

    发明人： ABE J ,  MATSUMOTO K ,  FUJII T ,  WATANABE T ,  YAMAGUCHI T

    IPC分类号： A61K31/545 ,  C07D501/18 ,  C12N9/80 ,  C12P35/02 ,  C12R1/11 ,  C12B1/00

    CPC分类号： C12N9/80 ,  C12P35/02 ,  Y10S435/815 ,  Y10S435/837

    摘要： D R A W I N G
    7-Amino desacetoxy cephalosporanic acid represented by the general formula,

    IS PRODUCED ACCORDING TO A PROCESS IN WHICH AN ALKALI METAL SALT OF A 7-ACYLAMINO DESACETOXY CEPHALOSPORANIC ACID REPRESENTED BY THE GENERAL FORMULA,
    WHEREIN R is a benzyl or phenoxymethyl group, and M is an alkali metal atom capable of forming a water-soluble salt, is treated with a carrier containing culture filtrate of an enzyme-producing strain belonging to the genus Bacillus which can decompose the amide bond of the said compound, said strain being Bacillus megaterium B-400 FERM-P No. 748, or is treated in an aqueous medium with an enzyme preparation containing said enzymeproducing strain.