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428 条记录 (耗时 0.016 秒)

    Determination of enzyme substrate concentration
    1.
    发明授权
    Determination of enzyme substrate concentration 失效
    酶底物浓度的测定

    公开(公告)号：US4218535A

    公开(公告)日：1980-08-19

    申请号：US967324

    申请日：1978-12-07

    申请人： Robert A. Ray

    发明人： Robert A. Ray

    IPC分类号： C12Q1/54 ,  C12Q1/58 ,  C12Q1/62 ,  G01N31/14 ,  C07G7/02

    CPC分类号： C12Q1/58 ,  C12Q1/54 ,  C12Q1/62

    摘要： An enzymatic reagent is provided for use in the kinetic measurement of the concentration of an enzyme substrate present in a sample and possessing the Michaelis-Menten relationship between reaction rate and substrate concentration. The reagent includes an enzyme such as glucose oxidase, uricase or urease for reacting with corresponding enzyme substrates such as glucose, uric acid or urea. In addition, the reagent includes a nonreacting competitive inhibitor of the enzyme. The inhibitor is present in an amount sufficient to improve the linearity of the reaction rate-concentration relationship associated with the enzyme substrate reaction at concentrations which are low relative to the Michaelis-Menten constant, Km, for the particular enzyme substrate reaction. The improved linearity, in turn, improves the accuracy of the kinetic measurements of substrate concentration made by linear output analyzers especially in an upper portion of the predetermined range where the relationship is particularly nonlinear.

    摘要翻译： 提供酶试剂用于动态测量样品中存在的酶底物的浓度，并具有反应速率和底物浓度之间的Michaelis-Menten关系。 试剂包括葡萄糖氧化酶，尿酸酶或脲酶等酶与葡萄糖，尿酸或尿素等相应的酶底物反应。 此外，试剂包括酶的非反应竞争性抑制剂。 抑制剂的存在量足以提高与酶底物反应相关的反应速率 - 浓度关系的线性关系，其浓度相对于特定酶底物反应的Michaelis-Menten常数Km为低。 改进的线性又提高了线性输出分析仪特别是在关系特别非线性的预定范围的上部的基底浓度的动力学测量的精度。

    Immobilization of an enzyme substrate
    2.
    发明授权
    Immobilization of an enzyme substrate 失效
    固定酶底物

    公开(公告)号：US4217415A

    公开(公告)日：1980-08-12

    申请号：US886504

    申请日：1978-03-14

    申请人： Raymond C. Barabino ,  Melvin H. Keyes

    发明人： Raymond C. Barabino ,  Melvin H. Keyes

    IPC分类号： C12N11/14 ,  B01D15/08 ,  C12Q1/34 ,  C12Q1/40 ,  C07G7/02 ,  C12D13/04 ,  G01N31/08 ,  G01N31/14

    CPC分类号： C12Q1/40 ,  B01D15/3814

    摘要： A carbohydrate substrate such as starch for a carbohydrate hydrolyzing enzyme is immobilized on a solid inorganic porous support to form a stable substrate-support composite useful in affinity chromatography and in methods where a precise amount of substrate is needed to perform an enzyme-substrate reaction to quantify the enzyme. The substrate may be activated with an agent such as cyanogen bromide or imidazole prior to deposition on the support so that it may be effectively modified while on the support. After deposition, the substrate is modified by reaction with an epoxyhalogen, aliphatic dihalide or aliphatic diamine to aid in holding it on the support. In an alternative embodiment, the carbohydrate, prior to deposition and modification on the support, is hydrolyzed with an enzyme, preferably dextranase.

    摘要翻译： 将碳水化合物底物如用于碳水化合物水解酶的淀粉固定在固体无机多孔载体上以形成可用于亲和层析的稳定的底物载体复合物，以及需要精确量的底物以进行酶 - 底物反应 量化酶。 在沉积到载体上之前，可以用诸如溴化氰或咪唑的试剂来活化底物，使得其可以在载体上被有效地改性。 沉积后，通过与环氧卤素，脂族二卤化物或脂族二胺的反应来改性底物以帮助将其保持在载体上。 在替代实施方案中，在载体上沉积和修饰之前，碳水化合物用酶优选葡聚糖酶水解。

    High loading of immobilized enzymes on activated carbon supports
    4.
    发明授权
    High loading of immobilized enzymes on activated carbon supports 失效
    固定化酶在活性炭载体上的高负载量

    公开(公告)号：US4204041A

    公开(公告)日：1980-05-20

    申请号：US838544

    申请日：1977-10-03

    申请人： James E. Bailey ,  Yong K. Cho

    发明人： James E. Bailey ,  Yong K. Cho

    IPC分类号： C12N11/06 ,  C12N11/14 ,  C07G7/02

    CPC分类号： C12N11/06 ,  C12N11/14

    摘要： Enzymes are immobilized on activated carbon supports at high load levels and with high stability by a procedure whereby a carbon support is first activated with a water-soluble carbodiimide derivative which forms a highly reactive intermediate with carboxyl and other active organic radicals on the surface of the carbon support; thereafter the complex of the carbon and carbodiimide is treated with an enzyme solution whereby the enzyme displaces the carbodiimide and forms a carbon-enzyme complex wherein the enzyme is immobilized and yet the carbon retains its surface activity. Preferred immobilizing agents are quaternary ammonium forms of aminocarbodiimides or hydrochloric acid salts of water-soluble carbodiimides. The immobilization of the enzymes on activated carbon provides materials which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

    摘要翻译： 酶以高负载水平固定在活性炭载体上，并具有高稳定性，其中碳载体首先用水溶性碳二亚胺衍生物活化，该衍生物在羧基和其它活性有机基团的表面上形成高反应性中间体 碳支持; 此后用酶溶液处理碳和碳二亚胺的络合物，由此酶置换碳二亚胺并形成酶固定化的碳 - 酶复合物，而碳保留其表面活性。 优选的固定剂是氨基碳二亚胺的季铵形式或水溶性碳二亚胺的盐酸盐。 将酶固定在活性炭上提供容易处理的物质，并且其被稳定以防止过氧化氢变性。

    Method of gelling microbial mycelia
    5.
    发明授权
    Method of gelling microbial mycelia 失效
    凝胶微生物菌丝体的方法

    公开(公告)号：US4184919A

    公开(公告)日：1980-01-22

    申请号：US891217

    申请日：1978-03-29

    申请人： Taisuke Iwasaki ,  Toshihiko Kikuchi

    发明人： Taisuke Iwasaki ,  Toshihiko Kikuchi

    IPC分类号： C12N11/04 ,  C13K11/00 ,  C07G7/02

    CPC分类号： C12N11/04 ,  C13K11/00

    摘要： The disclosed method involves adding a crosslinking reagent to mycelia which contain enzymes, freezing the mixture and then thawing the frozen mycelia. The resulting gelation immobilizes the intracellular enzyme in the mycelia. The gel may be used in the enzyme industry as a highly active enzyme preparation.

    摘要翻译： 所公开的方法包括向含有酶的菌丝体中加入交联剂，冷冻混合物，然后解冻冷冻的菌丝体。 所得凝胶化将细胞内酶固定在菌丝体中。 该凝胶可用作酶工业中的高活性酶制剂。

    Enzyme immobilization with a disulphide bridge
    6.
    发明授权
    Enzyme immobilization with a disulphide bridge 失效
    用二硫键固定酶

    公开(公告)号：US4176006A

    公开(公告)日：1979-11-27

    申请号：US869239

    申请日：1978-01-13

    申请人： Richard A. Cormier ,  Claude F. Gagnon

    发明人： Richard A. Cormier ,  Claude F. Gagnon

    IPC分类号： C12N11/06 ,  C12N11/08 ,  C12N11/14 ,  C12D13/00 ,  C07G7/02

    CPC分类号： C12N11/08 ,  C12N11/06 ,  C12N11/14

    摘要： Immobilized enzymes are provided in which the enzyme is covalently linked by a disulphide group-containing bridge to on inorganic or organic support carrier. Immobilizing by this method enables spent immobilized enzyme to be readily regenerated by reduction under mild conditions of the disulphide bridge to provide the carrier with mercaptan groups, and adding fresh enzyme to the carrier. The immobilization method is applicable to a wide variety of enzymes using the same chemical coupling reagents. The method is especially useful for immobilizing expensive enzymes such as glucose isomerase.

    摘要翻译： 提供了固定的酶，其中酶通过含二硫基团的桥共价连接到无机或有机载体载体上。 通过这种方法固定，可以通过在二硫键的温和条件下还原而使消耗的固定化酶容易地再生，从而为载体提供硫醇基团，并向载体中加入新鲜的酶。 固定化方法适用于使用相同化学偶联剂的各种酶。 该方法特别适用于固定昂贵的酶如葡萄糖异构酶。

    Analysis of lactic acid or lactate using lactate oxidase
    8.
    发明授权
    Analysis of lactic acid or lactate using lactate oxidase 失效
    使用乳酸氧化酶分析乳酸或乳酸

    公开(公告)号：US4166763A

    公开(公告)日：1979-09-04

    申请号：US749546

    申请日：1976-12-10

    申请人： Theordore W. Esders ,  Charles T. Goodhue ,  Richard M. Schubert

    发明人： Theordore W. Esders ,  Charles T. Goodhue ,  Richard M. Schubert

    IPC分类号： C12N9/02 ,  C12Q1/26 ,  G01N33/487 ,  G01N33/543 ,  C07G7/02 ,  G01N31/14

    CPC分类号： C12Q1/26 ,  Y10S435/805 ,  Y10S435/885

    摘要： Compositons and multilayer analytical elements comprising lactate oxidase which is substantially free of catalase and preferably produced by Streptococcus faecalis ATCC 12755 are provided for the quantitative analysis of lactic acid or lactate, especially in serum. The lactate oxidase catalyzes the reduction of lactic acid or lactate to pyruvate and hydrogen peroxide and the quantity of lactic acid or lactate is determined by detecting the amount of hydrogen peroxide produced. Preferably, the hydrogen peroxide is detected colorimetrically using a peroxidase-catalyzed detection system.

    摘要翻译： 提供了包含基本上不含过氧化氢酶并优选由粪肠球菌ATCC 12755产生的乳酸氧化酶的复合物和多层分析元件用于乳酸或乳酸盐，特别是在血清中的定量分析。 乳酸氧化酶催化乳酸或乳酸盐还原为丙酮酸盐和过氧化氢，乳酸或乳酸盐的量通过检测产生的过氧化氢的量来确定。 优选地，使用过氧化物酶催化的检测系统使用比色法检测过氧化氢。