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18 条记录 (耗时 0.021 秒)

    High loading of immobilized enzymes on activated carbon supports
    1.
    发明授权
    High loading of immobilized enzymes on activated carbon supports 失效
    固定化酶在活性炭载体上的高负载量

    公开(公告)号：US4289853A

    公开(公告)日：1981-09-15

    申请号：US147353

    申请日：1980-05-07

    申请人： James E. Bailey ,  Yong K. Cho

    发明人： James E. Bailey ,  Yong K. Cho

    IPC分类号： C12N11/06 ,  C12N11/14 ,  C12N11/02

    CPC分类号： C12N11/06 ,  C12N11/14

    摘要： Enzymes are immobilized on activated carbon supports at high load levels and with high stability by reacting an activated carbon support with a carbodiimide or an isoxazolium salt to form active organic radicals on the surface of the activated carbon support and thereafter treating the carbon support with an enzyme solution to immobilize the enzyme on the carbon support. The immobilization of enzymes on activated carbon provides immobilized enzymes which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

    摘要翻译： 酶通过活性炭载体与碳二亚胺或异恶唑盐反应而在高负载水平下固定在活性炭载体上并具有高稳定性，以在活性炭载体的表面上形成活性有机基团，然后用酶处理碳载体 溶液将酶固定在碳载体上。 酶在活性炭上的固定提供了易于处理的固定化酶，并且其被稳定以防止过氧化氢变性。

    High loading of immobilized enzymes on activated carbon supports
    2.
    发明授权
    High loading of immobilized enzymes on activated carbon supports 失效
    固定化酶在活性炭载体上的高负载量

    公开(公告)号：US4204041A

    公开(公告)日：1980-05-20

    申请号：US838544

    申请日：1977-10-03

    申请人： James E. Bailey ,  Yong K. Cho

    发明人： James E. Bailey ,  Yong K. Cho

    IPC分类号： C12N11/06 ,  C12N11/14 ,  C07G7/02

    CPC分类号： C12N11/06 ,  C12N11/14

    摘要： Enzymes are immobilized on activated carbon supports at high load levels and with high stability by a procedure whereby a carbon support is first activated with a water-soluble carbodiimide derivative which forms a highly reactive intermediate with carboxyl and other active organic radicals on the surface of the carbon support; thereafter the complex of the carbon and carbodiimide is treated with an enzyme solution whereby the enzyme displaces the carbodiimide and forms a carbon-enzyme complex wherein the enzyme is immobilized and yet the carbon retains its surface activity. Preferred immobilizing agents are quaternary ammonium forms of aminocarbodiimides or hydrochloric acid salts of water-soluble carbodiimides. The immobilization of the enzymes on activated carbon provides materials which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

    摘要翻译： 酶以高负载水平固定在活性炭载体上，并具有高稳定性，其中碳载体首先用水溶性碳二亚胺衍生物活化，该衍生物在羧基和其它活性有机基团的表面上形成高反应性中间体 碳支持; 此后用酶溶液处理碳和碳二亚胺的络合物，由此酶置换碳二亚胺并形成酶固定化的碳 - 酶复合物，而碳保留其表面活性。 优选的固定剂是氨基碳二亚胺的季铵形式或水溶性碳二亚胺的盐酸盐。 将酶固定在活性炭上提供容易处理的物质，并且其被稳定以防止过氧化氢变性。

    Expression of oxygen-binding proteins in plants
    3.
    发明授权
    Expression of oxygen-binding proteins in plants 失效
    植物中氧结合蛋白的表达

    公开(公告)号：US5959187A

    公开(公告)日：1999-09-28

    申请号：US720260

    申请日：1996-09-26

    申请人： James E. Bailey ,  Leif Bulow

    发明人： James E. Bailey ,  Leif Bulow

    IPC分类号： A01H5/00 ,  C07K14/805 ,  C12N1/15 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  C12P21/02 ,  C12R1/91 ,  C12N5/14

    CPC分类号： C12N15/8243 ,  C07K14/805 ,  C12N15/8257 ,  C12N15/8261 ,  C12N15/8267

    摘要： The present invention relates to genetic-engineering of plants for enhanced oxygen assimilation and utilization. More particularly, this invention relates to producing transgenic plants engineered to express oxygen-binding proteins such as, for example, hemoglobin, myoglobin, and hemoproteins. The engineered plants of the invention achieve quicker germination, are faster growing or maturing crops, produce higher crop yields, and/or contain higher levels of desired plant metabolites, particularly alkaloids.

    摘要翻译： 本发明涉及用于提高氧同化和利用的植物的遗传工程。 更具体地，本发明涉及生产工程化以表达氧结合蛋白例如血红蛋白，肌红蛋白和血红蛋白的转基因植物。 本发明的工程植物实现更快的萌发，更快的生长或成熟的作物，产生较高的作物产量，和/或含有较高水平的所需植物代谢物，特别是生物碱。

    Lift assembly for lowrider model cars
    4.
    发明授权
    Lift assembly for lowrider model cars 失效
    升降机总成为低级车型

    公开(公告)号：US5334077A

    公开(公告)日：1994-08-02

    申请号：US980302

    申请日：1992-11-23

    申请人： James E. Bailey

    发明人： James E. Bailey

    IPC分类号： A63H17/26

    CPC分类号： A63H17/262

    摘要： In a scale model of a lowrider vehicle including a chassis and first and second spaced axles supported on the chassis, a lift assembly includes the chassis being provided with guide structure for maintaining the transverse orientation of the first axle while allowing movement of the axle relative to the chassis within a plane perpendicular to the central longitudinal axis of the chassis. A lifting assembly is provided on the chassis for lifting the chassis relative to the axle between a lowered position and a raised position so as to simulate lifting and lowering movements of the modeled lowrider vehicle. In this manner, it is possible to simulate front and back, side-to-side and hopping moves conventionally performed with actual lowrider vehicles.

    摘要翻译： 在包括底盘以及支撑在底盘上的第一和第二间隔轴的低速车辆的比例模型中，提升组件包括底盘，该升降组件具有用于保持第一轴的横向定位的引导结构，同时允许轴相对于 底盘在垂直于机箱中心纵轴的平面内。 提升组件设置在底盘上，用于在下降位置和升高位置之间相对于车轴提升底盘，以便模拟建模的低速行驶车辆的提升和降低运动。 以这种方式，可以模拟通常用实际的低车辆进行的前后，侧向和跳跃。

    Enhancement of cell growth by expression of a cloned hemoglobin gene
    5.
    发明授权
    Enhancement of cell growth by expression of a cloned hemoglobin gene 失效
    通过表达克隆的血红蛋白基因增强细胞生长

    公开(公告)号：US5049493A

    公开(公告)日：1991-09-17

    申请号：US342451

    申请日：1989-01-24

    申请人： Chaitan S. Khosla ,  James E. Bailey

    发明人： Chaitan S. Khosla ,  James E. Bailey

    IPC分类号： C07K14/805 ,  C12N1/21 ,  C12N15/31 ,  C12N15/67 ,  C12N15/70

    CPC分类号： C12N15/67 ,  C07K14/805 ,  C12N15/70 ,  Y10S435/802 ,  Y10S435/804 ,  Y10S435/813 ,  Y10S435/818

    摘要： The invention relates to nucleotide sequences, including a substantially purified gene which codes for an oxygen-binding protein, and a gene promoter/regulator which is useful in subjecting the translation/transcription of DNA sequences to selective regulation by external control, and plasmid vectors containing those nucleotide sequences, which are valuable bioprocessing catalysts for enhancing the growth characteristics of cells, and increasing production of various proteins and metabolites of those cells. Methods for the use of these nucleotide sequences and related plasmids for a range of applications including oxygen supply to cells, growth enhancement, expression of various gene products, enhancement of oxygen-requiring processes, binding and separation of oxygen from liquids and gases, and a range of oxidative reactions are also disclosed.

    摘要翻译： PCT No.PCT / US88 / 03745 Sec。 一九八九年一月二十四日 102（e）日期1989年1月24日PCT提交1988年10月21日PCT公布。 WO89 / 03883 PCT出版物 日本1989年5月5日。本发明涉及核苷酸序列，包括编码氧结合蛋白的基本上纯化的基因，以及可用于使DNA序列的翻译/转录经外部选择性调节的基因启动子/调节子 对照和含有这些核苷酸序列的质粒载体，其是用于增强细胞生长特性的有价值的生物处理催化剂，并增加这些细胞的各种蛋白质和代谢物的产生。 将这些核苷酸序列和相关质粒用于一系列应用的方法，包括向细胞供氧，生长增强，各种基因产物的表达，氧需求过程的增强，氧与液体和气体的结合和分离，以及 还公开了氧化反应的范围。

    Cross-regulation of gene expression in recombinant cells
    8.
    发明授权
    Cross-regulation of gene expression in recombinant cells 失效
    重组细胞中基因表达的交叉调控

    公开(公告)号：US5416008A

    公开(公告)日：1995-05-16

    申请号：US770100

    申请日：1991-10-01

    申请人： James E. Bailey ,  Wilfred Chen ,  Pauli Kallio

    发明人： James E. Bailey ,  Wilfred Chen ,  Pauli Kallio

    IPC分类号： C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12N1/19 ,  C12N1/21 ,  C12N15/09

    CPC分类号： C12N15/635 ,  C12N15/70 ,  C12N15/81

    摘要： Recombinant cells providing for the controlled expression of product proteins by way of cross-regulation between interacting operons. A structural gene for a product protein and a structural gene for a repressor of a second operon are included in a first operon. A protein encoded by a structural gene of the second operon is a repressor of the first operon. The second operon may reside on a plasmid or a chromosome of the host cell. The present invention provides for controlled expression of product protein over a range of copy numbers, as well as high transcription efficiency in the induced state. The invention includes methods for the controlled expression of product protein by recombinant cells.

    摘要翻译： 通过相互作用的操纵子之间的交叉调控提供产物蛋白的受控表达的重组细胞。 产物蛋白质的结构基因和第二操纵子的阻遏物的结构基因包括在第一操纵子中。 由第二操纵子的结构基因编码的蛋白质是第一操纵子的阻遏物。 第二个操纵子可以位于宿主细胞的质粒或染色体上。 本发明提供产品蛋白质在拷贝数范围内的受控表达，以及诱导状态下的高转录效率。 本发明包括通过重组细胞控制产物蛋白表达的方法。