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    REVERSIBLE NATURAL PRODUCT GLYCOSYLTRANSFERASE-CATALYZED REACTIONS, COMPOUNDS AND RELATED METHODS
    4.
    发明申请
    REVERSIBLE NATURAL PRODUCT GLYCOSYLTRANSFERASE-CATALYZED REACTIONS, COMPOUNDS AND RELATED METHODS 有权
    可逆天然产物糖基转移酶催化反应，化合物及相关方法

    公开(公告)号：US20150141294A1

    公开(公告)日：2015-05-21

    申请号：US14335067

    申请日：2014-07-18

    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

    Inventor： JON S. THORSON ,  CHANGSHENG ZHANG ,  BYRON R. GRIFFITH

    IPC: C12P19/62

    CPC classification number: C12P19/623 ,  C07H15/203 ,  C07H17/08 ,  C07H19/01 ,  C07K9/008 ,  C12P19/18 ,  C12P19/44 ,  C12P19/62

    Abstract: The present invention relates to methods of use of glycosyltransferases and related novel compounds. The invention exploits the reversibility of glycosyltransferases to generate new sugars, unnatural biomolecules and numerous one-pot reactions for generation of new biomolecules having varied backbones such as enediynes, vancomycins, bleomycins, anthracyclines, macrolides, pluramycins, aureolic acids, indolocarbazoles, aminglycosides, glycopeptides, polyenes, coumarins, benzoisochromanequinones, calicheamicins, erythromycin, avermectins, ivermectins, angucyclines, cardiac glycosides, steroids or flavinoids. In preferred embodiments, the invention specifically relates to biosynthesis of anticancer (the enediyne calicheamicin, CLM), anthelmintic agents (the macrolides avermectin, ivermectin and erythromycin) and antibiotic (the glycopeptide vancomycin, VCM) natural product-based drugs developed by reversible, bidirectional glycosyltransferase-catalyzed reactions.

    Abstract translation: 本发明涉及使用糖基转移酶和相关新化合物的方法。 本发明利用糖基转移酶的可逆性来产生新的糖，非天然生物分子和许多一锅反应，用于产生具有不同骨架的新的生物分子，所述新生物分子具有不同的骨架，例如恩二烯，万古霉素，博来霉素，蒽环类抗生素，大环内酯类，阿拉伯霉素，阿拉伯酸，吲哚糖苷， ，多烯，香豆素，苯并二叠氮醌，加利车霉素，红霉素，阿凡曼菌素，伊维来菌素，角叉菜苷，强心苷，类固醇或黄素类。 在优选的实施方案中，本发明特别涉及抗逆转（双胍），抗胆碱药物（大环内酯阿维菌素，伊维菌素和红霉素）和抗生素（甘氨酸万古霉素，VCM）基于天然产物的药物的生物合成 糖基转移酶催化反应。

    Soybean-Based Fermentation Media, Methods of Making and Use
    5.
    发明申请
    Soybean-Based Fermentation Media, Methods of Making and Use 有权
    大豆发酵培养基，制作和使用方法

    公开(公告)号：US20130288308A1

    公开(公告)日：2013-10-31

    申请号：US13847091

    申请日：2013-03-19

    Applicant: Fermalogic, Inc.

    Inventor： J. Mark Weber

    IPC: C12P19/62

    CPC classification number: C12P19/62 ,  C12N1/38

    Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.

    Abstract translation: 提供了含有异黄酮贫乏豆粕或异黄酮贫乏豆粕产品的发酵介质和包含微生物生长底物的至少一种外源添加成分。 还提供了制备含有异黄酮贫乏大豆粉或异黄酮贫乏豆粕产品的发酵培养基的方法和获得发酵产物的方法。 本发明还涉及通过培养基和方法获得的发酵肉汤。 本发明还涉及由通过该方法获得的发酵肉汤产生的饲料添加剂。

    ENHANCING SPINOSYN PRODUCTION WITH OXYGEN BINDING PROTEINS
    7.
    发明申请
    ENHANCING SPINOSYN PRODUCTION WITH OXYGEN BINDING PROTEINS 有权
    用氧气结合蛋白提高胶原蛋白的生产

    公开(公告)号：US20120282657A1

    公开(公告)日：2012-11-08

    申请号：US13100202

    申请日：2011-05-03

    Applicant: Lei Han ,  Nigel Mouncey

    Inventor： Lei Han ,  Nigel Mouncey

    IPC: C12P19/62 ,  C40B30/00 ,  C12N1/21 ,  C12N15/63

    CPC classification number: C12P19/62 ,  C07K14/195 ,  C07K14/805

    Abstract: The invention describes the integration of polynucleotides into chromosomal DNA of S. spinosa species, which are useful for the production of insecticides, integrants thereof, and also to the use of the integrants. The invention includes the stable integration and expression of an oxygen-binding protein, VHb, which results in increased spinosyn production.

    Abstract translation: 本发明描述了将多核苷酸整合到刺螺菌属的染色体DNA中，其可用于生产杀虫剂，其整合剂，以及对整合体的使用。 本发明包括稳定整合和表达氧结合蛋白VHb，其导致多杀菌素产生增加。

    NRPS-PKS Gene Cluster and its Manipulation and Utility
    8.
    发明申请
    NRPS-PKS Gene Cluster and its Manipulation and Utility 有权
    NRPS-PKS基因簇及其操作和实用性

    公开(公告)号：US20110117606A1

    公开(公告)日：2011-05-19

    申请号：US12922718

    申请日：2009-03-20

    Applicant: Hanne Jørgensen ,  Trond Erling Ellingsen ,  Kristin Fløgstad Degnes ,  Per Bruheim ,  Håvard Sletta ,  Espen Fjaervik ,  Geir Klinkenberg ,  Sergey Zotchev

    Inventor： Hanne Jørgensen ,  Trond Erling Ellingsen ,  Kristin Fløgstad Degnes ,  Per Bruheim ,  Håvard Sletta ,  Espen Fjaervik ,  Geir Klinkenberg ,  Sergey Zotchev

    IPC: C12P19/34 ,  C07H21/04 ,  C12N9/00 ,  C12P7/26 ,  C12P17/10 ,  C12N1/00 ,  C12N1/21 ,  C12N1/20

    CPC classification number: C12N15/52 ,  C07D225/02 ,  C07K14/36 ,  C12P17/10 ,  C12P19/62 ,  C12R1/465

    Abstract: The present invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 1; or (b) a nucleotide sequence which is the complement of SEQ ID No. 1; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 1; or (d) a nucleotide sequence having at least 85% sequence identity (preferably at least 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity) with SEQ ID No. 1; or (e) a part of any one of (a) to (d), wherein said nucleic acid molecule encodes or is a complementary to a nucleic acid molecule encoding one or more polypeptides, or comprises or is complementary to a nucleic acid molecule comprising one or more genetic elements, having functional activity in the synthesis of a polyketide-based or macrolactam molecule. Particularly the invention contemplates the modification of the nucleic acid of the invention, encoding the biosynthetic machinery for the synthesis of the polyketide macrolactam BE-14106, including expressing in a microorganism the modified nucleic acid molecule. In certain aspects the modification includes introducing, mutating, deleting, replacing or inactivating a sequence encoding one or more activities or proteins encoded by said nucleic acid molecule. Other aspects of the invention include a microorganism containing the modified and unmodified nucleic acid and recovering the polyketide-based or macrolactam molecule from said mircoorganism.

    Abstract translation: 本发明提供了一种核酸分子，其包含：（a）SEQ ID No.1所示的核苷酸序列; 或（b）作为SEQ ID No.1的互补序列的核苷酸序列; 或（c）与SEQ ID No.1退行的核苷酸序列; 或（d）具有至少85％序列同一性（优选至少87％，90％，91％，92％，93％，94％，95％，96％，97％，98％或99％ 序列同一性）; 或（e）（a）至（d）中任一项的一部分，其中所述核酸分子编码或与编码一种或多种多肽的核酸分子互补，或包含或互补于包含 一种或多种在合成聚酮化合物或大内酰胺分子中具有功能活性的遗传元件。 特别地，本发明考虑了本发明的核酸的修饰，其编码用于合成聚酮化物大内酰胺BE-14106的生物合成机理，包括在微生物中表达经修饰的核酸分子。 在某些方面，修饰包括引入，突变，缺失，替换或失活编码由所述核酸分子编码的一种或多种活性或蛋白质的序列。 本发明的其它方面包括含有经修饰和未修饰的核酸的微生物，并从所述微生物中回收聚酮化合物或大环内酰胺分子。

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