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    Novel maltose dehydrogenase, process for its production, and analytical method using the same
    1.
    发明授权
    Novel maltose dehydrogenase, process for its production, and analytical method using the same 失效
    新型麦芽糖脱氢酶，其生产方法和使用其的分析方法

    公开(公告)号：US4683198A

    公开(公告)日：1987-07-28

    申请号：US674009

    申请日：1984-11-23

    申请人： Hidehiko Ishikawa ,  Kazuo Matsuura ,  Hideo Misaki

    发明人： Hidehiko Ishikawa ,  Kazuo Matsuura ,  Hideo Misaki

    IPC分类号： C12N9/04 ,  C12Q1/32 ,  C12R1/44 ,  C12Q1/40

    CPC分类号： C12N9/0006 ,  C12Q1/32 ,  Y10S435/882 ,  Y10S435/883 ,  Y10S435/884

    摘要： An enzyme which acts on a reducing terminal of a monosaccharide or oligosaccharide without requiring NAD or NADP and which catalyzes the reaction ##STR1## wherein R is a saccharide chain residue or hydrogen, A is a hydrogen acceptor other than NAD or NADP, AH or AHn is a reduced form acceptor and n is 1 or 2. This maltose dehydrogenase is produced by culturing a microorganism belonging to genus Staphylococcus, specifically, sp. B-0875 FERM BP-385, and isolating the thus-produced maltose dehydrogenase from the culture medium. An assay method for the determination of saccharide or the activity of a saccharide liberating enzyme, comprises reacting this enzyme with a substrate in the presence of a hydrogen acceptor, and measuring the amount of a detectable change.

    摘要翻译： 作用于单糖或寡糖的还原末端而不需要NAD或NADP并且催化反应的酶，其中R是糖链残基或氢，A是NAD以外的氢受体或 NADP，AH或AHn是还原型受体，n是1或2.这种麦芽糖脱氢酶是通过培养属于葡萄球菌属的微生物，特别是sp。 B-0875FERM BP-385，并从培养基中分离由此产生的麦芽糖脱氢酶。 用于测定糖类或糖释放酶活性的测定方法包括在氢受体存在下使该酶与底物反应，并测量可检测的变化量。

    Process for production of ascorbate oxidase
    2.
    发明授权
    Process for production of ascorbate oxidase 失效
    生产抗坏血酸氧化酶的方法

    公开(公告)号：US4331763A

    公开(公告)日：1982-05-25

    申请号：US217178

    申请日：1980-12-16

    申请人： Eiji Matsumura ,  Hidehiko Ishikawa ,  Hideo Misaki

    发明人： Eiji Matsumura ,  Hidehiko Ishikawa ,  Hideo Misaki

    IPC分类号： C12N9/02 ,  C12N9/04

    CPC分类号： C12Y110/03003 ,  C12N9/0063

    摘要： Ascorbate oxidase is produced by extraction from plants of the genus Sechium, particularly the species thereof which is Sechium edule Sw. The crushed plant tissues are extracted with an aqueous alkaline solvent, preferably at about pH 11. The extract is then subjected to centrifugation, concentration under vacuum, salting out with ammonium sulfate and solvent fractionation with acetone, to prepare crude ascorbate oxidase, which is then further purified by dialysis, ion exchange chromatography, adsorption chromatography and gel filtration. The ascorbate oxidase thus obtained has an optimum pH of about 7, a km value of 0.3 mM, an isoelectric point of around pH 6.3 and a molecular weight of about 100,000.

    摘要翻译： 抗坏血酸氧化酶通过从Sechium属植物中提取而产生，特别是Sechium edule Sw的种类。 粉碎的植物组织用碱性水溶液萃取，优选在约pH 11下。然后将提​​取物进行离心，在真空下浓缩，用硫酸铵盐析并用丙酮溶剂分级，制备粗抗坏血酸氧化酶 进一步通过透析，离子交换层析，吸附层析和凝胶过滤纯化。 由此获得的抗坏血酸氧化酶具有约7的最佳pH，0.3mM的km值，约6.3的等电点和约100,000的分子量。

    Color printing method
    3.
    发明授权
    Color printing method 失效
    彩色打印方式

    公开(公告)号：US4182560A

    公开(公告)日：1980-01-08

    申请号：US895441

    申请日：1978-04-11

    申请人： Masanobu Oguchi ,  Hidehiko Ishikawa ,  Haruyoski Okuyama ,  Yasuo Uchida ,  Kiyoshi Izawa

    发明人： Masanobu Oguchi ,  Hidehiko Ishikawa ,  Haruyoski Okuyama ,  Yasuo Uchida ,  Kiyoshi Izawa

    IPC分类号： G03B17/24 ,  G03B27/73 ,  G03C5/02 ,  G03B27/32 ,  G03B27/76

    CPC分类号： G03B27/73 ,  G03B17/24 ,  G03C5/02 ,  G03B2206/004 ,  G03B2217/243 ,  G03B2217/246

    摘要： In a color printing method, a color photosensitive material is exposed in the photographing step to object illuminating light, which is recorded thereon as an optical density by photographic treatments. In the printing step, the optical density is detected to determine blue, green and red exposures, which cause the object illuminating light to be reproduced on a color positive photosensitive material so as to have neutral gray or to be colored to a standard color. An object image on the color photosensitive material is printed on the color positive photosensitive material with the most suitable color reproduction with the same three-color component exposures as those determined in printing the object illuminating light.

    摘要翻译： 在彩色打印方法中，在拍摄步骤中曝光彩色感光材料，以照相光照射作为光密度记录在其上的照明光。 在打印步骤中，检测光密度以确定蓝色，绿色和红色曝光，这导致物体照明光在彩色正性感光材料上再现以具有中性灰色或被着色为标准颜色。 彩色感光材料上的物体图像被印刷在具有与打印物体照明光中确定的相同的三色成分曝光的最合适的颜色再现的彩色正性感光材料上。

    Assay method for amylase activity and method of producing maltose dehydrogenase for use therein
    5.
    发明授权
    Assay method for amylase activity and method of producing maltose dehydrogenase for use therein 失效
    淀粉酶活性的测定方法和生产用于其中的麦芽糖脱氢酶的方法

    公开(公告)号：US4427771A

    公开(公告)日：1984-01-24

    申请号：US311263

    申请日：1981-10-14

    申请人： Hideo Misaki ,  Eiji Muramatsu ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    发明人： Hideo Misaki ,  Eiji Muramatsu ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    IPC分类号： C12N9/04 ,  C12Q1/40 ,  C12N1/20 ,  C12Q1/32 ,  C12R1/11

    CPC分类号： C12N9/0006 ,  C12Q1/40 ,  G01N2400/18 ,  Y10S435/837

    摘要： An assay method for amylase activity in a biological specimen such as serum, saliva or urine. The enzyme amylase in the specimen is used to decompose a substrate which is a glucose polymer having a modified reducing terminal glucose residue or a cyclic glucose polymer. A component of the decomposed substrate is measured as an indication of amylase activity in the specimen. The residue may be amylose, amylopectin, starch, starch hydrolyzate, an etherified reducing terminal, an esterified reducing terminal, gluconolactone or a gluconic acid residue or its derivative. Decomposed substrate assay may be effected by contacting the same with maltose dehydrogenase and NAD or NADP, whereupon the assay is performed by measuring the amount of reduced NAD or reduced NADP, by reacting the same with reduced-form hydrogen transport colorimetric reaction reagent. This reagent may be a tetrazolium salt and diaphorase, or tetrazolium salt and phenazinemethosulfate. To remove pre-existing glucose and maltose present in the specimen, the specimen may be pretreated with alpha-glucosidase or kinase in the presence of Mg.sup.++ and ATP, the kinase being for example hexokinase. The preferred maltose dehydrogenase is produced by culturing Bacillus megaterium B-0779 FERM-P No. 5662.

    摘要翻译： 生物样品如血清，唾液或尿液中淀粉酶活性的测定方法。 试样中的酶淀粉酶用于分解具有修饰的还原性末端葡萄糖残基或环状葡萄糖聚合物的葡萄糖聚合物的底物。 测量分解的底物的组分作为样品中淀粉酶活性的指示。 残基可以是直链淀粉，支链淀粉，淀粉，淀粉水解物，醚化还原末端，酯化还原末端，葡萄糖酸内酯或葡萄糖酸残基或其衍生物。 分解的底物测定可以通过使其与麦芽糖脱氢酶和NAD或NADP接触来实现，因此通过使其与还原形式的氢转运比色反应试剂反应来测量还原的NAD或还原的NADP的量来进行测定。 该试剂可以是四唑盐和心律黄素，或四唑鎓盐和吩嗪甲硫酸盐。 为了除去样品中存在的现有葡萄糖和麦芽糖，可以在Mg ++和ATP存在下用α-葡糖苷酶或激酶预处理样品，所述激酶例如己糖激酶。 优选的麦芽糖脱氢酶是通过培养巨大芽孢杆菌B-0779 FERM-P No.5662产生的。

    Light-sensitive silver halide photographic materials
    8.
    发明授权
    Light-sensitive silver halide photographic materials 失效
    感光卤化银照相材料

    公开(公告)号：US4108668A

    公开(公告)日：1978-08-22

    申请号：US792125

    申请日：1977-04-29

    申请人： Kaiichiro Sakazume ,  Noboru Fujimori ,  Eiichi Sakamoto ,  Hidehiko Ishikawa

    发明人： Kaiichiro Sakazume ,  Noboru Fujimori ,  Eiichi Sakamoto ,  Hidehiko Ishikawa

    IPC分类号： G03C1/18

    CPC分类号： G03C1/18

    摘要： Novel light-sensitive silver halide photographic materials comprising at least one sensitizing dye having the following formula: ##STR1## wherein Z represents a non-metallic atom group necessary for completing a three membered heterocyclic ring, R represents an alkyl or a substituted alkyl radical, R.sub.1 and R.sub.2 represent hydrogen atoms or lower alkyl radicals, R.sub.3 represents a hydrogen atom, an alkyl or alkoxyl radical, m represents an integer of 0 or 1, X represents an acid anion and forms an inner salt when m is 0, and n represents an integer of 1 or 2.

    摘要翻译： 新型感光卤化银照相材料，其包含至少一种具有下式的致敏染料：其中Z表示完成三元杂环所必需的非金属原子团，R表示烷基或取代的烷基， R1和R2表示氢原子或低级烷基，R3表示氢原子，烷基或烷氧基，m表示0或1的整数，X表示酸阴离子，当m为0时形成内盐，n表示 1或2的整数。

    Assay method using NAD synthetase
    9.
    发明授权
    Assay method using NAD synthetase 失效
    使用NAD合成酶的测定方法

    公开(公告)号：US5206146A

    公开(公告)日：1993-04-27

    申请号：US481752

    申请日：1990-02-15

    申请人： Hideo Misaki ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    发明人： Hideo Misaki ,  Hidehiko Ishikawa ,  Kazuo Matsuura

    IPC分类号： C12N9/00 ,  C12Q1/00 ,  C12Q1/25 ,  C12Q1/48 ,  C12Q1/50

    CPC分类号： C12N9/93 ,  C12Q1/008 ,  C12Q1/25 ,  C12Q1/48 ,  C12Q1/50 ,  Y10S435/836

    摘要： An assay method for a component in a specimen containing any one of ATP, deamide-NAD and an amide donor which comprises performing a main reaction which comprises incubating the specimen with NAD synthetase in the presence of ATP, deamide-NAD, an amide donor and Mg.sup.++ to generate NAD; performing a coenzyme cycling reaction by combining the oxidation-reduction reaction system with coenzyme NAD and the oxidation-reduction reaction system with coenzyme reduced NAD, and measuring a consumed or generated component in the cycling reaction. The NAD synthetase can be produced by culturing the microorganism Bacillus licheniformis B-0844 FERM P-6809, in a culture medium, and isolating the thus-produced NAD synthetase therefrom.

    摘要翻译： 含有ATP，脱酰胺-NAA和酰胺供体中的任一种的样品中的组分的测定方法，其包括进行主要反应，其包括在ATP，脱酰胺-NAD，酰胺供体的存在下将样品与NAD合成酶孵育， Mg ++生成NAD; 通过将氧化还原反应体系与辅酶NAD和氧化还原反应体系与辅酶还原NAD进行组合进行辅酶循环反应，测定循环反应中消耗或产生的成分。 NAD合成酶可以通过在培养基中培养微生物地衣芽孢杆菌B-0844 FERM P-6809，并从其中分离由此产生的NAD合成酶来制备。