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    Highly-Sensitive Genomic Assays Employing Chimeric Bacteriophage Standards
    3.
    发明申请
    Highly-Sensitive Genomic Assays Employing Chimeric Bacteriophage Standards 审中-公开
    采用嵌合噬菌体标准的高度敏感的基因组测定

    公开(公告)号：US20080318204A1

    公开(公告)日：2008-12-25

    申请号：US10497828

    申请日：2002-12-04

    申请人： Linqi Zhang ,  David D. Ho

    发明人： Linqi Zhang ,  David D. Ho

    IPC分类号： C12Q1/70 ,  C12N7/00

    CPC分类号： C12N7/00 ,  C12N2730/10122 ,  C12N2795/14111 ,  C12N2795/14143 ,  C12Q1/706 ,  C12Q2561/113 ,  C12Q2545/114 ,  C12Q2545/101

    摘要： Methods are provided for sensitively quantitating at least one pre-selected DNA sequence in a biological sample utilizing hybridization methodology, the method employing as an internal standard an infectious bacteriophage particle comprising a detectable target DNA sequence other than that present in the pre-selected DNA sequence or in DNA quantitated from the biological sample, and as an external standard, an infectious bacteriophage particle comprising at least the pre-selected DNA sequence.

    摘要翻译： 提供了使用杂交方法灵敏定量生物样品中至少一个预先选择的DNA序列的方法，该方法使用包含可检测的靶DNA序列的感染性噬菌体颗粒作为内标，所述感染性噬菌体颗粒除了存在于预先选择的DNA序列之外 或在生物样品中定量的DNA中，并且作为外部标准，是至少包含预先选择的DNA序列的感染性噬菌体颗粒。

    Method of recovering a nucleic acid encoding a proteinaceous binding domain which binds a target material
    4.
    发明授权
    Method of recovering a nucleic acid encoding a proteinaceous binding domain which binds a target material 有权
    回收编码结合目标材料的蛋白质结合结构域的核酸的方法

    公开(公告)号：US07118879B2

    公开(公告)日：2006-10-10

    申请号：US10126544

    申请日：2002-04-22

    申请人： Robert Charles Ladner ,  Sonia Kosow Guterman ,  Bruce Lindsay Roberts ,  William Markland ,  Arthur Charles Ley ,  Rachel Baribault Kent

    发明人： Robert Charles Ladner ,  Sonia Kosow Guterman ,  Bruce Lindsay Roberts ,  William Markland ,  Arthur Charles Ley ,  Rachel Baribault Kent

    IPC分类号： C12Q1/68

    CPC分类号： C40B40/02 ,  A61K38/00 ,  C07K1/047 ,  C07K1/107 ,  C07K14/43522 ,  C07K14/805 ,  C07K14/8114 ,  C07K14/8117 ,  C12N7/00 ,  C12N15/1037 ,  C12N2795/14111 ,  C12N2795/14122 ,  C12N2795/14143

    摘要： In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.

    摘要翻译： 为了获得针对所选靶标的新型结合蛋白，每个编码包含相似潜在结合结构域的家族之一的蛋白质的DNA分子和要求在选择的细菌细胞的外表面上显示蛋白质的结构信号 ，细菌孢子或噬菌体（遗传包装）被引入到遗传包装中。 蛋白质被表达，并且潜在的结合结构域显示在包装的外表面上。 分离和扩增具有识别靶分子的结合域的细胞或病毒。 然后表征成功的结合结构域。 将这些成功结合结构域中的一个或多个用作设计新的潜在结合结构域家族的模型，并重复该过程，直到获得对靶分子具有所需亲和力的新结合结构域。 在一个实施方案中，第一潜在结合域家族与牛胰蛋白酶抑制剂有关，遗传包是M13噬菌体，蛋白质包括M13基因III蛋白的外表面转运信号。

    Directed evolution of novel binding proteins
    5.
    发明申请
    Directed evolution of novel binding proteins 失效
    新型结合蛋白的定向进化

    公开(公告)号：US20030113717A1

    公开(公告)日：2003-06-19

    申请号：US09893878

    申请日：2001-06-29

    发明人： Robert Charles Ladner ,  Sonia Kosow Guterman ,  Bruce Lindsay Roberts ,  William Markland ,  Arthur Charles Ley ,  Rachel Baribault Kent

    IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  C12P019/34 ,  C12N015/87

    CPC分类号： C40B40/02 ,  A61K38/00 ,  C07K1/047 ,  C07K1/107 ,  C07K14/43522 ,  C07K14/805 ,  C07K14/8114 ,  C07K14/8117 ,  C12N7/00 ,  C12N15/1037 ,  C12N2795/14111 ,  C12N2795/14122 ,  C12N2795/14143

    摘要： In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural-signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.

    摘要翻译： 为了获得针对所选靶标的新型结合蛋白，每个编码包含相似潜在结合结构域家族之一的蛋白质的DNA分子以及呼吁在所选细菌的外表面上显示蛋白质的结构信号 细胞，细菌孢子或噬菌体（遗传包装）被引入到遗传包装中。 蛋白质被表达，并且潜在的结合结构域显示在包装的外表面上。 分离和扩增具有识别靶分子的结合域的细胞或病毒。 然后表征成功的结合结构域。 将这些成功结合结构域中的一个或多个用作设计新的潜在结合结构域家族的模型，并重复该过程，直到获得对靶分子具有所需亲和力的新结合结构域。 在一个实施方案中，第一潜在结合域家族与牛胰蛋白酶抑制剂有关，遗传包是M13噬菌体，蛋白质包括M13基因III蛋白的外表面转运信号。

    NOVEL PHAGE DISPLAY VECTOR
    9.
    发明申请
    NOVEL PHAGE DISPLAY VECTOR 有权
    新款PHAGE显示矢量图

    公开(公告)号：US20120322678A1

    公开(公告)日：2012-12-20

    申请号：US13510148

    申请日：2010-11-18

    申请人： Peter Lowe ,  Sven Berger

    发明人： Peter Lowe ,  Sven Berger

    IPC分类号： C12N15/70 ,  C12N15/71 ,  C40B30/04 ,  C12N15/72 ,  C40B40/02 ,  C40B50/06

    CPC分类号： C12N15/1037 ,  C12N15/86 ,  C12N2795/14143

    摘要： The invention relates to a minimized phage display vector comprising at least a cloning cassette, a phage display cassette and a bacterial cassette, said cloning cassette comprising a nucleic acid sequence encoding a polypeptide corresponding at least to the intra-domain loop of a constant domain of an antibody. It also relates to their uses for the production of libraries of phages, each phage expressing on its surface a binding protein to be screened for its capacity to bind a binding partner.

    摘要翻译： 本发明涉及包含至少一个克隆盒，噬菌体展示盒和细菌盒的最小化的噬菌体展示载体，所述克隆盒包含编码多肽的核酸序列，所述核酸序列至少对应于恒定结构域的恒定结构域的域内环 抗体。 它还涉及其用于生产噬菌体文库的用途，每个噬菌体在其表面上表达一种结合蛋白质，以筛选其结合配偶体的结合能力。