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    Method For Production Of Large Numbers Of Cartilage Cells With Phenotype Retention
    3.
    发明申请
    Method For Production Of Large Numbers Of Cartilage Cells With Phenotype Retention 审中-公开
    用于生产具有表型保留性的大量软骨细胞的方法

    公开(公告)号：US20150344847A1

    公开(公告)日：2015-12-03

    申请号：US14290438

    申请日：2014-05-29

    申请人： Theodore I Malinin

    发明人： Theodore I Malinin

    IPC分类号： C12N5/077

    CPC分类号： C12N5/0655 ,  C12N2533/18 ,  C12N2533/90

    摘要： A method for production of large numbers of cartilage cells with phenotype retention intended for treatment of articular cartilage lesions or preparing viable cartilage tissue by propagation of chondrocytes from cartilage explants, human or animal, with the retention of phenotypes of the cartilage cells, in which cells are chondrocytes retaining morphologic attributes of the same or chondrocyte progenitor cells. The method includes culturing the cells in which cultured cells are organized into mature hyaline cartilage on the surface of biologic structures such as cancellous or cortical bone lamina. The method for culturing chondrocytes to produce three dimensional cellular structures consisting of cartilage cells and cell produced extracellular cartilage matrix.

    摘要翻译： 用于生产具有表型保留的大量软骨细胞的方法，其用于治疗关节软骨损伤或通过软骨移植物，人或动物的软骨细胞的增殖来制备活的软骨组织，其中保留了软骨细胞的表型，其中细胞 是软骨细胞保留相同或软骨细胞祖细胞的形态特征。 该方法包括将培养细胞组织成生物结构如松质骨或皮质骨层的表面的成熟透明软骨培养。 培养软骨细胞以产生由软骨细胞和细胞产生的细胞外软骨基质组成的三维细胞结构的方法。

    Culture substrate and culture method for undifferentiated cell and undifferentiated cultured cell
    4.
    发明授权
    Culture substrate and culture method for undifferentiated cell and undifferentiated cultured cell 有权
    未分化细胞和未分化培养细胞的培养底物和培养方法

    公开(公告)号：US09096826B2

    公开(公告)日：2015-08-04

    申请号：US11561055

    申请日：2006-11-17

    申请人： Fumihiko Kitagawa ,  Takafumi Imaizumi ,  Katsunori Sasaki

    发明人： Fumihiko Kitagawa ,  Takafumi Imaizumi ,  Katsunori Sasaki

    IPC分类号： C12N5/07 ,  C12M3/00 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/0797

    CPC分类号： C12N5/0068 ,  C12N5/0606 ,  C12N2533/14 ,  C12N2533/18

    摘要： A culture substrate made of ceramics of any one or more of titania, alumina, zirconia, yttria, and carbon is used where minute pores having a pore diameter of from 0.1 μm to 10 μm are provided at least for a place to which a cell is seeded, and an undifferentiated cell is seeded to at least one place of a surface of the culture substrate, whereby a culture substrate and a culture method for efficiently culturing the undifferentiated cell to be formed into a 3-dimensional cell block shape without causing immune rejection, and an undifferentiated cultured cell.

    摘要翻译： 使用由氧化钛，氧化铝，氧化锆，氧化钇和碳中的任何一种或多种的陶瓷制成的培养基底，其中至少在细胞为该细胞的位置处提供孔径为0.1μm至10μm的细孔 接种，并将未分化细胞接种至培养基材表面的至少一处，由此培养基质和培养方法，用于有效培养未分化细胞形成3维细胞块形状而不引起免疫排斥 ，和未分化培养细胞。

    Tooth scaffolds
    5.
    发明授权
    Tooth scaffolds 有权
    牙脚架

    公开(公告)号：US08979534B2

    公开(公告)日：2015-03-17

    申请号：US13378789

    申请日：2010-06-17

    申请人： Jeremy J. Mao

    发明人： Jeremy J. Mao

    IPC分类号： A61C13/08 ,  A61L27/48 ,  A61L27/46 ,  A61L27/54 ,  A61L27/56 ,  C12N5/077 ,  A61C8/00 ,  A61K35/12

    CPC分类号： A61L27/48 ,  A61C8/0036 ,  A61K35/12 ,  A61L27/46 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/252 ,  A61L2300/414 ,  A61L2300/426 ,  A61L2300/45 ,  A61L2300/622 ,  A61L2430/12 ,  C12N5/0654 ,  C12N2501/155 ,  C12N2501/21 ,  C12N2533/18 ,  C12N2533/40 ,  C12N2533/54 ,  C08L67/04

    摘要： Provided is an acellular mammalian tooth-shaped scaffold including a compound that is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic. Also provided is a method of replacing a tooth in the mouth of a mammal, where the tooth is absent and a tooth socket is present in the mouth at the position of the absent tooth. The method includes implanting an acellular scaffold having the shape of the missing tooth into the tooth socket. Additionally, a method of making a tooth scaffold is provided. The method includes synthesizing an acellular scaffold in the shape of a mammalian tooth and adding at least one compound that is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic.

    摘要翻译： 提供了一种无细胞哺乳动物的牙齿支架，其包括具有趋化性，成骨性，牙质发生，萌生或成骨的化合物。 还提供了一种替代牙齿不存在的口腔中的牙齿，并且在缺牙处的位置处存在牙槽的方法。 该方法包括将具有缺牙的形状的无细胞支架植入牙槽。 另外，提供了制造牙齿支架的方法。 该方法包括合成哺乳动物牙齿形状的无细胞支架，并添加至少一种化学趋化，成骨，牙质，萌发或成骨的化合物。

    Materials and methods for cryopreserved bone constructs
    8.
    发明授权
    Materials and methods for cryopreserved bone constructs 有权
    低温保存骨构建体的材料和方法

    公开(公告)号：US08367059B2

    公开(公告)日：2013-02-05

    申请号：US13184357

    申请日：2011-07-15

    申请人： Teng Ma

    发明人： Teng Ma

    IPC分类号： A01N63/00 ,  C12N11/02 ,  C12N5/00

    CPC分类号： C12N5/0068 ,  A61K2035/124 ,  A61L27/12 ,  A61L27/26 ,  A61L27/3834 ,  A61L27/3847 ,  C12N5/0663 ,  C12N2533/18 ,  C12N2533/54 ,  C12N2533/72 ,  C08L5/08 ,  C08L89/06

    摘要： Porous HCG scaffolds are provided in a perfusion bioreactor. Cells are seeded in the HCG scaffolds and cell culture media is perfused through the bioreactor to allow for cell seeding and growth. The cell culture media is removed, the HCG-cell constructs washed, and then preserved in the bioreactor with a perfusion comprising cryopreservation fluid comprising one or more of DMSO, trehalose, glycerol, ethylene glycol, or serum. The HCG-cell constructs (or the perfusion chambers containing them) are then removed from the bioreactor and placed in a cryopreservant media and maintained at about −80° C. The frozen HCG-cell constructs (or the chambers containing them) can then be stored at a suitable cryogenic temperature until needed. When needed, frozen HCG-cell constructs can be removed from cold storage and thawed using suitable means.

    摘要翻译： 在灌注生物反应器中提供多孔HCG支架。 将细胞接种在HCG支架中，并通过生物反应器灌注细胞培养基以允许细胞接种和生长。 洗涤细胞培养基，洗涤HCG细胞构建体，然后用包含DMSO，海藻糖，甘油，乙二醇或血清中的一种或多种的冷冻保存液的灌注保存在生物反应器中。 然后将HCG细胞构建体（或含有它们的灌注室）从生物反应器中移出并置于冷冻保存的培养基中并保持在约-80℃。然后可以将冷冻的HCG-细胞构建体（或含有它们的室） 储存在合适的低温温度下直至需要。 当需要时，可以使用合适的方法将冷冻的HCG细胞构建体从冷藏中移出并解冻。

    ENGINEERED OSTEOCHONDRAL CONSTRUCT FOR TREATMENT OF ARTICULAR CARTILAGE DEFECTS
    10.
    发明申请
    ENGINEERED OSTEOCHONDRAL CONSTRUCT FOR TREATMENT OF ARTICULAR CARTILAGE DEFECTS 审中-公开
    用于治疗关节缺损的工程化骨科结构

    公开(公告)号：US20100241228A1

    公开(公告)日：2010-09-23

    申请号：US12696366

    申请日：2010-01-29

    申请人： Carina Syring ,  Rudiger Walter Arthur von Versen

    发明人： Carina Syring ,  Rudiger Walter Arthur von Versen

    IPC分类号： A61F2/02 ,  C12N5/02

    CPC分类号： A61K35/32 ,  A61K35/12 ,  A61L27/3608 ,  A61L27/3654 ,  A61L27/3683 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2533/18

    摘要： An implant for articular cartilage repair includes (1) a three-dimensional body formed of cancellous bone having a demineralized section that contains bone morphogenetic proteins (BMP's) that are released by the demineralization but retained in the body, and (2) a cartilage layer formed on a surface of the demineralized section. The cartilage layer is formed by a method including the steps of (a) isolating chondrocytes from articular cartilage of a donor; (b) cultivating the isolated chondrocytes in a medium; (c) adding the cultivated chondrocytes to the demineralized section of the body, whereby the cultivated chondrocytes are stimulated by the BMP's retained in the body; and (d) incubating the cultivated chondrocytes to form a plurality of layers of chondrocytes on the demineralized section, wherein the plurality of layers of chondrocytes forms the cartilage layer.

    摘要翻译： 用于关节软骨修复的植入物包括（1）由松质骨形成的三维体，其具有通过脱矿质释放但保留在体内的骨形态发生蛋白（BMP）的软化部分，和（2）软骨层 形成在软化部分的表面上。 通过包括以下步骤的方法形成软骨层：（a）从供体的关节软骨中分离软骨细胞; （b）在培养基中培养分离的软骨细胞; （c）将培养的软骨细胞添加到身体的软化部分，由此培养的软骨细胞被保留在体内的BMP刺激; 和（d）培养培养的软骨细胞以在软化部分上形成多层软骨细胞，其中多层软骨细胞形成软骨层。