公开(公告)号：US20240327459A1

公开(公告)日：2024-10-03

申请号：US18744868

申请日：2024-06-17

申请人： Follicum AB

发明人： Jan Alenfall ,  Maria Ekblad ,  Pontus Dunér

IPC分类号： C07K7/06 ,  A61K38/00 ,  A61P3/08 ,  C07K7/08

CPC分类号： C07K7/06 ,  A61P3/08 ,  C07K7/08 ,  A61K38/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/705

摘要： The present disclosure concerns agents and their use in the treatment of endocrine, nutritional and/or metabolic diseases in a mammal. The disclosure furthermore concerns novel peptide fragments.

公开(公告)号：US11697819B2

公开(公告)日：2023-07-11

申请号：US16437844

申请日：2019-06-11

申请人： Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.

发明人： Oded Shoseyov ,  Helena Magrisso ,  Tzvi Zvirin ,  Amit Yaari ,  Zohar Katz

IPC分类号： C12N15/82 ,  C12N15/62 ,  C12P21/00 ,  C07K16/22 ,  C07K16/24 ,  C12N9/24

CPC分类号： C12N15/8258 ,  C07K16/22 ,  C07K16/241 ,  C12N9/2402 ,  C12N15/62 ,  C12N15/625 ,  C12N15/8205 ,  C12N15/8218 ,  C12N15/8257 ,  C12P21/005 ,  C12Y302/01037 ,  C12Y302/01051 ,  C07K2317/13 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/705

摘要： A method of modifying a glycosylation pattern of a polypeptide-of-interest in a plant or plant cell is provided. The method comprising expressing in a plant or plant cell transformed to express at least one glycosidase in a subcellular compartment, a nucleic acid sequence encoding the polypeptide-of-interest, such that the at least one glycosidase and the polypeptide-of-interest are co-localized to the subcellular compartment of the plant or plant cell, thereby modifying the glycosylation pattern of the polypeptide-of-interest in the plant or plant cell.

公开(公告)号：US11649265B2

公开(公告)日：2023-05-16

申请号：US16606601

申请日：2018-04-30

申请人： AgroSpheres, Inc.

发明人： Ameer Hamza Shakeel ,  Sepehr Zomorodi ,  Joseph Thomas Frank ,  Zachery George Davis ,  Payam Pourtaheri

IPC分类号： A01N25/26 ,  A01N25/28 ,  C07K14/195 ,  A01N63/22 ,  C07K14/32 ,  C07K14/335 ,  C07K14/34 ,  C12N1/08 ,  C12N9/54 ,  A01N63/50 ,  C12N15/62 ,  C12N9/52 ,  A61K39/108 ,  B01F101/04

CPC分类号： C07K14/195 ,  A01N25/28 ,  A01N63/22 ,  A01N63/50 ,  C07K14/32 ,  C07K14/335 ,  C07K14/34 ,  C12N1/08 ,  C12N9/52 ,  C12N9/54 ,  C12N15/62 ,  A61K39/0258 ,  B01F2101/04 ,  C07K2319/705 ,  A01N63/50 ,  A01N63/27 ,  A01N47/06 ,  A01N47/24 ,  A01N47/36 ,  A01N51/00

摘要： The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

公开(公告)号：US10005820B2

公开(公告)日：2018-06-26

申请号：US13397313

申请日：2012-02-15

申请人： Matthew J. Giacalone ,  Michael J. Newman

发明人： Matthew J. Giacalone ,  Michael J. Newman

IPC分类号： C07K14/195 ,  C12N1/02 ,  C12N1/20 ,  C12N15/00 ,  C12N15/10 ,  C07K16/00 ,  C12N15/70 ,  C12N15/74

CPC分类号： C07K14/195 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/705 ,  C12N1/02 ,  C12N1/20 ,  C12N15/00 ,  C12N15/1037 ,  C12N15/70 ,  C12N15/74

摘要： The present application relates to the use of bacterial minicells as targeted delivery agents in vivo and in vitro. Described herein are genetically engineered eubacterial minicells designed to preferentially target and deliver therapeutically relevant agents using a minicell surface coupling molecule capable of binding and displaying antibodies or other Fc-containing targeting moiety fusions and conjugates.

公开(公告)号：US20160202251A1

公开(公告)日：2016-07-14

申请号：US14897274

申请日：2014-08-01

申请人： BEACLE INC.

发明人： Yasumasa GOH ,  Yasunori ODA

IPC分类号： G01N33/543 ,  G01N33/544 ,  C07K14/195 ,  C07K14/31 ,  C07K14/315 ,  G01N33/569 ,  C07K14/005

CPC分类号： G01N33/54346 ,  C07K14/005 ,  C07K14/195 ,  C07K14/31 ,  C07K14/315 ,  C07K2319/705 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2760/18823 ,  G01N33/544 ,  G01N33/56905 ,  G01N33/56966 ,  G01N33/56983 ,  G01N2333/02 ,  G01N2333/44 ,  G01N2333/71

摘要： An object of the present invention is to provide an immunoassay that has excellent detection sensitivity and that remarkably suppresses the detection background. The means for achieving the object is to provide a virus-like particle that contains a protein having self-organization ability, the particle being modified with a biologically active molecule at at least one cysteine residue of the protein having self-organization ability via a thiol group or thiol groups thereof.

摘要翻译： 本发明的目的是提供具有优异的检测灵敏度并显着抑制检测背景的免疫测定。 实现该目的的手段是提供一种含有具有自组织能力的蛋白质的病毒样颗粒，该颗粒在具有自组织能力的蛋白质的至少一个半胱氨酸残基上经生物活性分子修饰，具有硫醇 基团或硫醇基团。

公开(公告)号：US09011870B2

公开(公告)日：2015-04-21

申请号：US11989118

申请日：2006-07-20

申请人： Cornelis Johannes Leenhouts ,  Maarten Leonardus Van Roosmalen ,  Tjibbe Bosma

发明人： Cornelis Johannes Leenhouts ,  Maarten Leonardus Van Roosmalen ,  Tjibbe Bosma

IPC分类号： A61K39/385 ,  C07K14/335 ,  A61K39/00

CPC分类号： A61K47/48369 ,  A61K39/385 ,  A61K47/68 ,  A61K2039/541 ,  A61K2039/55555 ,  A61K2039/6056 ,  A61K2039/6068 ,  A61K2039/622 ,  C07K14/335 ,  C07K16/18 ,  C07K2319/70 ,  C07K2319/705

摘要： The invention relates to the areas of immunology and vaccine delivery. More specifically, it relates to a bacterial vaccine delivery technology with built-in immunostimulatory properties which allows the immobilization of any antigen of interest, without prior antigen modification. Provided is an antigen-loaded immunogenic carrier complex comprising at least one bifunctional polypeptide attached to an immunogenic carrier, said bifunctional polypeptide comprising a peptidoglycan binding domain (PBD) through which the polypeptide is attached to said carrier, fused to an antigen binding domain (ABD) to which at least one antigen of interest is bound. Also provided is a pharmaceutical (e.g. vaccine) composition comprising an antigen-loaded immunogenic carrier complex of the invention.

摘要翻译： 本发明涉及免疫学和疫苗递送领域。 更具体地说，本发明涉及具有内在免疫刺激性质的细菌疫苗递送技术，其允许固定目的抗原，而无需先前的抗原修饰。 本发明提供了一种抗原负载的免疫原性载体复合物，其包含至少一个与免疫原性载体连接的双功能多肽，所述双功能多肽包含肽聚糖结合结构域（PBD），多肽与所述载体连接，融合至抗原结合结构域 ），至少一种目标抗原结合于该抗原。 还提供了包含本发明的抗原负载的免疫原性载体复合物的药物（例如疫苗）组合物。

公开(公告)号：US09006395B2

公开(公告)日：2015-04-14

申请号：US12900214

申请日：2010-10-07

申请人： Clifford Charles Shone ,  Keith Alan Foster ,  John Chaddock ,  Philip Marks ,  J. Mark Sutton ,  Patrick Stancombe ,  Jonathan Wayne

发明人： Clifford Charles Shone ,  Keith Alan Foster ,  John Chaddock ,  Philip Marks ,  J. Mark Sutton ,  Patrick Stancombe ,  Jonathan Wayne

IPC分类号： A61K38/00 ,  A61K39/08 ,  A61K47/48 ,  C07K14/33 ,  C07K16/12 ,  C12N9/52 ,  C12N15/62 ,  A61K39/00

CPC分类号： A61K39/08 ,  A61K38/00 ,  A61K39/00 ,  A61K47/6415 ,  A61K47/646 ,  A61K2039/505 ,  A61K2039/53 ,  A61K2039/627 ,  C07K14/33 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/23 ,  C07K2319/50 ,  C07K2319/55 ,  C07K2319/705 ,  C07K2319/75 ,  C12N9/52 ,  C12N15/62 ,  Y10S530/825

摘要： A single polypeptide is provided which comprises first and second domains. The first domain enables the polypeptide to cleave one or more vesicle or plasma-membrane associated proteins essential to exocytosis, and the second domain enables the polypeptide to be translocated into a target cell or increases the solubility of the polypeptide, or both. The polypeptide thus combines useful properties of a clostridial toxin, such as a botulinum or tetanus toxin, without the toxicity associated with the natural molecule. The polypeptide can also contain a third domain that targets it to a specific cell, rendering the polypeptide useful in inhibition of exocytosis in target cells. Fusion proteins comprising the polypeptide, nucleic acids encoding the polypeptide and methods of making the polypeptide are also provided. Controlled activation of the polypeptide, is possible and the polypeptide can be incorporated into vaccines and toxin assays.

摘要翻译： 提供了包含第一和第二结构域的单个多肽。 第一结构域使多肽能够切割一种或多种与胞吐作用相关的囊泡或质膜相关的蛋白质，而第二结构域使多肽易位于靶细胞或增加多肽或两者的溶解度。 因此，该多肽结合梭菌毒素如肉毒杆菌或破伤风毒素的有用性质，而不与天然分子相关的毒性。 多肽还可以含有将其靶向特定细胞的第三结构域，使得多肽可用于抑制靶细胞中的胞吐作用。 还提供了包含多肽的融合蛋白，编码多肽的核酸和制备多肽的方法。 多肽的受控活化是可能的，并且多肽可以并入疫苗和毒素测定中。

公开(公告)号：US09005992B2

公开(公告)日：2015-04-14

申请号：US13051219

申请日：2011-03-18

申请人： Hyung Joon Cha ,  Chang Sup Kim

发明人： Hyung Joon Cha ,  Chang Sup Kim

IPC分类号： G01N33/543 ,  G01N33/531 ,  G01N21/65 ,  C07K14/435

CPC分类号： G01N33/54353 ,  C07K14/43504 ,  C07K2319/21 ,  C07K2319/705 ,  G01N21/658 ,  G01N33/531

摘要： The present invention relates to a novel fusion protein comprising Staphylococcal protein A and mussel adhesive protein, a biochip comprising a solid substrate to which the fusion protein is attached, and a method for detecting a target antigen in a biological sample using the biochip. Furthermore, the present invention relates to a polynucleotide encoding the fusion protein, a recombinant vector comprising the polynucleotide, a transformed cell comprising the recombinant vector, and a method of preparing the fusion protein by transformed cell comprising the recombinant vector.

摘要翻译： 本发明涉及包含葡萄球菌蛋白A和贻贝粘合蛋白的新型融合蛋白，包含与融合蛋白连接的固体底物的生物芯片，以及使用生物芯片检测生物样品中的靶抗原的方法。 此外，本发明涉及编码融合蛋白的多核苷酸，包含多核苷酸的重组载体，包含重组载体的转化细胞，以及通过包含重组载体的转化细胞制备融合蛋白的方法。

公开(公告)号：US20110189159A1

公开(公告)日：2011-08-04

申请号：US13122513

申请日：2009-10-02

申请人： Deb Chatterjee ,  Stanislaw Jan Kaczmarcyk

发明人： Deb Chatterjee ,  Stanislaw Jan Kaczmarcyk

IPC分类号： A61K38/48 ,  C12N9/48 ,  C07H21/00 ,  C12P21/00 ,  C12N5/10 ,  C12N15/63 ,  C07K14/00 ,  C07K7/06 ,  A61P35/00

CPC分类号： C07K14/005 ,  A61K47/6901 ,  C07K14/00 ,  C07K14/245 ,  C07K2319/09 ,  C07K2319/10 ,  C07K2319/50 ,  C07K2319/705 ,  C07K2319/735 ,  C12N7/00 ,  C12N2740/13043 ,  C12N2760/20222 ,  C12N2760/20223 ,  C12N2810/6072 ,  C12N2810/6081

摘要： The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.

摘要翻译： 本发明提供蛋白质递送的方法和组合物。 本发明具有病毒样颗粒，制备病毒样颗粒的方法和使用病毒样颗粒将蛋白质递送至细胞的方法，以提供蛋白质治疗和治疗疾病或病症。 本发明还涉及将蛋白质靶向细胞的方法，蛋白质治疗方法以及使用从全长TUS鉴定的TUS蛋白，NLS或NES来治疗疾病或病症的方法。

公开(公告)号：USRE41277E1

公开(公告)日：2010-04-27

申请号：US12285368

申请日：2008-10-02

申请人： Arne Forsgren

发明人： Arne Forsgren

IPC分类号： A61K39/102

CPC分类号： A61K39/102 ,  A61K38/00 ,  A61K39/00 ,  C07K14/285 ,  C07K16/1242 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/705 ,  Y10T436/116664

摘要： A novel surface exposed protein of Haemophilus influenzae or related Haemophilus species is described. The protein named protein D is an Ig receptor for human IgD and has an apparent molecular weight of 42,000. Protein D can be detected in all of 116 encapsulated and non-encapsulated isolates of H. influenzae studied. The protein from all strains shows in addition to the same apparent molecular weight immunogenic similarities since protein D from all strains interacts with three different mouse monoclonal antibodies and monoclonal human IgD. A method for purification of protein D is described. Cloning of the protein D gene from H. influenzae in E. coli is described as well as the nucleotide sequence and the deduced amino acid sequence.

摘要翻译： 描述了流感嗜血杆菌或相关嗜血杆菌物种的新型表面暴露蛋白。 称为蛋白D的蛋白质是人IgD的Ig受体，其表观分子量为42,000。 蛋白D可以在所研究的流感嗜血杆菌的所有116个包封和非包囊的分离物中检测到。 来自所有菌株的蛋白质除了具有相同的表观分子量的免疫原性相似性之外还显示出，因为来自所有菌株的蛋白质D与三种不同的小鼠单克隆抗体和单克隆人IgD相互作用。 描述了蛋白D的纯化方法。 描述大肠杆菌中流感嗜血杆菌蛋白D基因的克隆以及核苷酸序列和推导的氨基酸序列。