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    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand
    5.
    发明授权
    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand 有权
    对免疫球蛋白具有亲和力的蛋白质和免疫球蛋白结合亲和配体

    公开(公告)号：US09403883B2

    公开(公告)日：2016-08-02

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/31 ,  C07K14/47 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。

    Mutated immunoglobulin-binding protein
    8.
    发明授权
    Mutated immunoglobulin-binding protein 有权
    突变的免疫球蛋白结合蛋白

    公开(公告)号：US09156892B2

    公开(公告)日：2015-10-13

    申请号：US13488662

    申请日：2012-06-05

    申请人： Sophia Hober

    发明人： Sophia Hober

    IPC分类号： B01J20/286 ,  C07K1/22 ,  C07K14/195 ,  C07K14/31 ,  B01D15/38 ,  B01J20/32 ,  C07K16/06

    CPC分类号： B01D15/3809 ,  B01J20/24 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3244 ,  B01J2220/44 ,  B01J2220/52 ,  C07K1/22 ,  C07K14/001 ,  C07K14/195 ,  C07K14/31 ,  C07K16/065 ,  Y10S530/81 ,  Y10S530/825

    摘要： The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

    摘要翻译： 本发明涉及一种免疫球蛋白结合蛋白，其中至少一种天冬酰胺残基已被突变为除谷氨酰胺或天冬氨酸以外的氨基酸，该突变赋予pH值高达约13-14的化学稳定性增加 到亲本分子。 蛋白质可以例如衍生自能够与互补决定区（CDR）如蛋白A，优选葡萄球菌蛋白A的B结构域结合的免疫球蛋白分子的其它区域的蛋白质。本发明还涉及 用于亲和力分离的基质，其包含免疫球蛋白结合蛋白作为与固体支持物偶联的配体，其中蛋白质配体至少一种天冬酰胺残基已被突变为除谷氨酰胺以外的氨基酸。