公开(公告)号：US12226561B2

公开(公告)日：2025-02-18

申请号：US17671353

申请日：2022-02-14

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： Lars Norderhaug ,  Angel Varela-Rohena ,  Nephi D. Jones

IPC: A61M1/36 ,  B01L3/00 ,  B03C1/01 ,  B03C1/28 ,  B03C1/30 ,  C07K16/28 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M3/00

Abstract: A magnetic particle separation system includes a magnetic field generating device having an upper surface with a receiving area formed thereon; and a magnetic field generating element disposed beneath the upper surface, the magnetic field generating element being configured to produce a magnetic field above the upper surface. A container assembly is disposed on the upper surface and includes: a flexible container having an outer wall with an interior surface that at least partially bounds an internal compartment, the outer wall having a front side and an opposing back side with the internal compartment disposed therebetween; a fluid inlet extending through the outer wall at the front side; a fluid outlet extending through the outer wall at the front side; and a first partition projecting into the internal compartment from the front side between the fluid inlet and the fluid outlet.

公开(公告)号：US12209239B2

公开(公告)日：2025-01-28

申请号：US17320572

申请日：2021-05-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Todd Peterson ,  Axel Trefzer ,  Thomas Poehmerer

IPC: C12N15/10 ,  B01J19/00 ,  C12N15/64 ,  C12N15/66 ,  C12P19/34

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20250019864A1

公开(公告)日：2025-01-16

申请号：US18606205

申请日：2024-03-15

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C07K14/725 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20250019752A1

公开(公告)日：2025-01-16

申请号：US18706621

申请日：2022-11-02

Applicant: Life Technologies Corporation

Inventor： Daniel Philippe Blanchard ,  Maria Cinta Gomez Silvan

IPC: C12Q1/6848 ,  C12Q1/686

Abstract: This disclosure describes master mix compositions, nucleic acid amplification kits, methods of manufacturing master mix compositions, and methods of using master mix compositions that minimize or eliminate the negative effects of contaminant nucleic acids. Conventional master mix compositions often include contaminant nucleic acids.

公开(公告)号：US20250011863A1

公开(公告)日：2025-01-09

申请号：US18769897

申请日：2024-07-11

Applicant: Life Technologies Corporation

Inventor： Dumitru Brinza ,  Zheng Zhang ,  Fiona Hyland ,  Rajesh Gottimukkala

IPC: C12Q1/6874 ,  G16B5/00 ,  G16B5/20 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. A list of probable variants can be provided.

公开(公告)号：US12188000B2

公开(公告)日：2025-01-07

申请号：US18317036

申请日：2023-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nephi D. Jones ,  Christopher D. Brau

IPC: B01D46/00 ,  B01D5/00 ,  B01D19/00 ,  B01D46/44 ,  B01D46/58 ,  B01D53/26 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36

Abstract: A method for filtering a gas includes delivering a gas into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; and regulating the application of the partial vacuum based on a pressure reading of the gas upstream or downstream of the gas filter assembly.

公开(公告)号：US20240426775A1

公开(公告)日：2024-12-26

申请号：US18732345

申请日：2024-06-03

Applicant: Life Technologies Corporation

Inventor： James BUSTILLO ,  Wolfgang HINZ ,  Kim JOHNSON ,  Jonathan ROTHBERG

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/423 ,  H01L29/78

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：USD1053799S1

公开(公告)日：2024-12-10

申请号：US29738833

申请日：2020-06-19

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC (SHANGHAI) INSTRUMENTS CO. LTD.

Designer： Xin Mathers ,  Guomin (Jimmy) Zheng

公开(公告)号：US20240392226A1

公开(公告)日：2024-11-28

申请号：US18248428

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Inventor： Morten LUHR ,  Michael GORDON ,  Ming Tiong SIA ,  Kok Shyong CHONG ,  Woon Liang Terence SOH ,  Seng Leong LIM ,  Ida Caroline SCHROEDER ,  Katrine ALFSNES ,  Terry JianHui LIM ,  Marie BOSNES ,  Axl Alois NEURAUTER ,  Ketil Winther PEDERSEN ,  Autumn Day WILBUR ,  Sean ZIMMERMAN

IPC: C12M1/12 ,  C12M1/00 ,  G01N35/00

Abstract: A bead processing assembly for use in attaching magnetic beads to biological cells and other biological materials and/or separating magnetic beads from biological cells and other biological materials includes a base assembly having a housing, a support panel disposed on the housing and having a front face, a first pinch valve at least partially outwardly projecting from the front face of the support panel, and a first pump at least partially outwardly projecting from the front face of the support panel. A rocker assembly is supported on the base assembly and includes a mount assembly supported on the base assembly, a platform assembly pivotably secured to the mount assembly, and a rocker drive for repeatedly rocking the platform assembly relative to the mount assembly.

公开(公告)号：US12146189B2

公开(公告)日：2024-11-19

申请号：US16433380

申请日：2019-06-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Hubbell

IPC: C40B50/16 ,  C12Q1/6869 ,  C12Q1/6874 ,  C40B20/04 ,  C40B70/00 ,  G16B30/00 ,  G16C20/64 ,  G16C99/00

Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.