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公开(公告)号：US20240025939A1
公开(公告)日：2024-01-25
申请号：US17909091
申请日：2021-03-05
Inventor： Robert POTTER , Nikolai NETUSCHIL
Abstract: The present disclosure generally relates to compositions and methods for the synthesis of nucleic acid molecules with low error rates. Provided, as examples, are compositions and methods for high throughput synthesis and assembly of nucleic acid molecules, in many instances, with high sequence fidelity. In many instances, thermostable mismatch recognition proteins (e.g., thermostable mismatch binding protein, thermostable mismatch endonucleases) will be present in compositions and use methods provided.
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公开(公告)号：US20220090161A1
公开(公告)日：2022-03-24
申请号：US17459053
申请日：2021-08-27
Inventor： Claudia CHIOCCHINI , Axel TREFZER , Krishna VATTEM , Phillip KUHN
IPC: C12P21/02 , C12M1/36 , C12N15/10 , C12N15/11 , C12N15/67 , C12P13/00 , C12P19/34 , C12Q1/6809 , C40B50/04
Abstract: The present disclosure generally relates to devices, compositions and methods for designing and producing nucleic acid molecules and the production of encoded proteins using these nucleic acid molecules. In some aspect, the disclosure relates to automation for the in vitro generation of coding DNA molecules, the in vitro transcription of these DNA molecules to generate protein coding RNA molecules, and the in vitro translation of these protein coding RNA molecules to produce proteins.
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公开(公告)号：US11041068B2
公开(公告)日：2021-06-22
申请号：US16305372
申请日：2017-06-07
Inventor： Kristian Andersson , Geir Fonnum , Korbinian Heil
IPC: C08L25/14 , C08L25/08 , B01J19/00 , C08F212/08 , C08F220/58 , C08F293/00
Abstract: This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide-oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.
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公开(公告)号：US20230278039A1
公开(公告)日：2023-09-07
申请号：US18040500
申请日：2021-08-06
Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH
Inventor： Ansgar ROMIG , Alexander LOCHNER , Mark KERZHNER
CPC classification number: B01L9/527 , B01L3/502738 , B01L3/502715 , B01L2200/027 , B01L2200/10 , B01L2300/047 , B01L2400/0487 , B01L2400/0644 , B01L2300/0609 , B01L2300/042 , B01L2200/0689
Abstract: The present invention relates to devices, system and methods for synthesizing and post-processing nucleic acids. The system comprising a fluid supply unit, a synthesis unit comprising a microfluidic chip, configured for the synthesis of nucleic acids, a valve assembly, comprising at least one multiport valve, and a collection unit configured to collect nucleic acids. The method for synthesising nucleic acids utilizes the system and comprises synthesising nucleic acids on the microfluidic chip, selectively releasing synthesised nucleic acids from the microfluidic chip, guiding released nucleic acids to the collection unit, and collecting released nucleic acids in a well plate comprised by the collection unit. The method for post-processing synthesized nucleic acids comprises providing one or more nucleic acids attached to a solid support in a first compartment, adding a first solution to cleave the nucleic acids off the support and adding a second solution to elute the nucleic acids into a second compartment.
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公开(公告)号：US20220033858A1
公开(公告)日：2022-02-03
申请号：US17327002
申请日：2021-05-21
Inventor： Namritha RAVINDER , Korbinian HEIL , Yizhu GUO , Xiquan LIANG , Robert POTTER , Sanjay KUMAR
Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.
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Patent Agency Ranking
公开(公告)号：US11046953B2
公开(公告)日：2021-06-29
申请号：US16678576
申请日：2019-11-08
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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公开(公告)号：US20180291413A1
公开(公告)日：2018-10-11
申请号：US15766172
申请日：2016-10-04
Inventor： Claudia CHIOCCHINI , Axel TREFZER , Krishna VATTEM , Phillip KUHN
IPC: C12P21/02 , C12N15/67 , C12P13/00 , C12N15/11 , C12N15/10 , C12Q1/6809 , C12M1/36 , C40B50/04 , C12P19/34
Abstract: The present disclosure generally relates to devices, compositions and methods for designing and producing nucleic acid molecules and the production of encoded proteins using these nucleic acid molecules. In some aspect, the disclosure relates to automation for the in vitro generation of coding DNA molecules, the in vitro transcription of these DNA molecules to generate protein coding RNA molecules, and the in vitro translation of these protein coding RNA molecules to produce proteins.
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公开(公告)号：US20240101999A1
公开(公告)日：2024-03-28
申请号：US18235205
申请日：2023-08-17
Applicant: Thermo Fisher Scientific GENEART GmbH , LIFE TECHNOLOGIES AS , LIFE TECHNOLOGIES CORPORATION
Inventor： Thomas POEHMERER , Phillip KUHN , Frank NOTKA , Andreas ZEIDLER , Korbinian HEIL , Axel TREFZER , Geir FONNUM , Federico KATZEN , Kristian ANDERSSON , Xiquan LIANG
CPC classification number: C12N15/101 , B01L3/502761 , B03C5/026 , C25B3/29 , C25B9/70 , C25B11/04 , C25B15/02 , B01J2219/00468 , B01J2219/005 , B01J2219/00653 , B01J2219/00695 , B01J2219/00722 , B01L3/502715 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0893 , B01L2400/046 , B01L2400/0487 , B03C2201/26
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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公开(公告)号：US10563240B2
公开(公告)日：2020-02-18
申请号：US14775648
申请日：2014-03-14
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer , Federico Katzen , Xiquan Liang
IPC: C12P19/34
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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公开(公告)号：US10519439B2
公开(公告)日：2019-12-31
申请号：US15463363
申请日：2017-03-20
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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