公开(公告)号：US20180291413A1

公开(公告)日：2018-10-11

申请号：US15766172

申请日：2016-10-04

Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH ,  PIERCE BIOTECHNOLOGY, INC.

Inventor： Claudia CHIOCCHINI ,  Axel TREFZER ,  Krishna VATTEM ,  Phillip KUHN

IPC: C12P21/02 ,  C12N15/67 ,  C12P13/00 ,  C12N15/11 ,  C12N15/10 ,  C12Q1/6809 ,  C12M1/36 ,  C40B50/04 ,  C12P19/34

Abstract: The present disclosure generally relates to devices, compositions and methods for designing and producing nucleic acid molecules and the production of encoded proteins using these nucleic acid molecules. In some aspect, the disclosure relates to automation for the in vitro generation of coding DNA molecules, the in vitro transcription of these DNA molecules to generate protein coding RNA molecules, and the in vitro translation of these protein coding RNA molecules to produce proteins.

公开(公告)号：US20220090161A1

公开(公告)日：2022-03-24

申请号：US17459053

申请日：2021-08-27

Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH ,  PIERCE BIOTECHNOLOGY, INC.

Inventor： Claudia CHIOCCHINI ,  Axel TREFZER ,  Krishna VATTEM ,  Phillip KUHN

IPC: C12P21/02 ,  C12M1/36 ,  C12N15/10 ,  C12N15/11 ,  C12N15/67 ,  C12P13/00 ,  C12P19/34 ,  C12Q1/6809 ,  C40B50/04

Abstract: The present disclosure generally relates to devices, compositions and methods for designing and producing nucleic acid molecules and the production of encoded proteins using these nucleic acid molecules. In some aspect, the disclosure relates to automation for the in vitro generation of coding DNA molecules, the in vitro transcription of these DNA molecules to generate protein coding RNA molecules, and the in vitro translation of these protein coding RNA molecules to produce proteins.

公开(公告)号：US20240101999A1

公开(公告)日：2024-03-28

申请号：US18235205

申请日：2023-08-17

Applicant: Thermo Fisher Scientific GENEART GmbH ,  LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas POEHMERER ,  Phillip KUHN ,  Frank NOTKA ,  Andreas ZEIDLER ,  Korbinian HEIL ,  Axel TREFZER ,  Geir FONNUM ,  Federico KATZEN ,  Kristian ANDERSSON ,  Xiquan LIANG

IPC: C12N15/10 ,  B01L3/00 ,  B03C5/02 ,  C25B3/29 ,  C25B9/70 ,  C25B11/04 ,  C25B15/02

CPC classification number: C12N15/101 ,  B01L3/502761 ,  B03C5/026 ,  C25B3/29 ,  C25B9/70 ,  C25B11/04 ,  C25B15/02 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C2201/26

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20220195417A1

公开(公告)日：2022-06-23

申请号：US17603394

申请日：2020-04-15

Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Phillip KUHN ,  Thomas HOFMEISTER ,  Nikolai NETUSCHIL ,  Joern PUETZSCHLER ,  Tatiana KONOVALOVA

IPC: C12N15/10

Abstract: Compositions and methods are provided herein for high throughput synthesis and assembly of nucleic acid molecules. Specific aspects include methods and rules for designing, grouping and pooling of nucleic acid molecules for efficient multiplex assembly, amplification, processing and analysis to obtain error-free assembly products. Provided compositions and methods allow for miniaturization, parallelization, high throughput production and scaling, and cost reduction in gene synthesis workflows.

公开(公告)号：US20180363037A1

公开(公告)日：2018-12-20

申请号：US15781899

申请日：2016-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Korbinian HEIL ,  Frank NOTKA ,  Kyle GEE ,  Jason DALLWIG ,  Phillip KUHN ,  Schuyler CORRY

IPC: C12Q1/6834

Abstract: This disclosure relates to methods, compositions, and related apparatuses for detection and quantification of nucleic acid molecules associated with a solid surface. Methods can include detecting or quantifying at least one nucleic acid molecule associated with a surface by contacting the at least one nucleic acid molecule with a fluorophore, wherein the fluorophore emits increased fluorescence at a wavelength when in contact with nucleic acid molecule, and detecting or measuring fluorescence from the fluorophore at the wavelength. Compositions can comprise a fluorophore and a nucleic acid molecule, wherein the nucleic acid molecule is associated with a surface, and the fluorophore has the property of emitting increased fluorescence at a wavelength when in contact with a nucleic acid molecule.