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    Cellular analysis using Raman surface scanning
    2.
    发明授权
    Cellular analysis using Raman surface scanning 有权
    使用拉曼表面扫描的细胞分析

    公开(公告)号：US07776547B2

    公开(公告)日：2010-08-17

    申请号：US11027470

    申请日：2004-12-30

    申请人： Mark Roth ,  Andrew Berlin ,  Selena Chan ,  Tae-Woong Koo ,  Xing Su ,  Lei Sun

    发明人： Mark Roth ,  Andrew Berlin ,  Selena Chan ,  Tae-Woong Koo ,  Xing Su ,  Lei Sun

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54366 ,  G01N33/54373 ,  G01N33/58 ,  Y10T436/10

    摘要： Methods and apparatus are provided for assaying cell samples, which may be living cells, using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs intrinsically produce SERS signals upon laser irradiation, making COIN-labeled probes particularly suitable in a variety of methods for assaying cells, including biological molecules that may be contained on or within cells, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Systems and chips useful for practicing the invention assays are also provided.

    摘要翻译： 提供了用于使用用复合有机 - 无机纳米颗粒（COIN）标记的探针和嵌入探针部分所连接的聚合物基质内的COIN的微球来测定可能为活细胞的细胞样品的方法和装置。 COIN在激光照射下固有地产生SERS信号，使得COIN标记的探针特别适用于多种测定细胞的方法，包括可能包含在细胞内或细胞内的生物分子，其中大部分不是固有的拉曼活性的。 本发明提供使用特异性和简并结合的夹心免疫测定的变体，组织样品和细胞微结构的反相测定方法，溶液置换和竞争测定等。 还提供了用于实施本发明测定的系统和芯片。

    ENCAPSULATION OF RAMAN ACTIVE NANOPARTICLES
    4.
    发明申请
    ENCAPSULATION OF RAMAN ACTIVE NANOPARTICLES 审中-公开
    拉曼活性纳米颗粒的包封

    公开(公告)号：US20080241569A1

    公开(公告)日：2008-10-02

    申请号：US11694293

    申请日：2007-03-30

    申请人： Haoyu Qin ,  Jingwu Zhang ,  Claire E. Dentinger ,  Lei Sun ,  Xing Su

    发明人： Haoyu Qin ,  Jingwu Zhang ,  Claire E. Dentinger ,  Lei Sun ,  Xing Su

    IPC分类号： B32B5/16

    CPC分类号： G01N21/658 ,  B82Y15/00 ,  B82Y30/00 ,  Y10T428/12028 ,  Y10T428/2991

    摘要： Embodiments of the present invention provide methods for coating nanoparticles with polymeric coatings and nanoparticles that are coated with polymeric coatings. The polymeric coatings typically comprise two or more layers wherein the first layer has a charge that is opposite to that of the second layer. In further embodiments, the nanoparticles that can act as labels or reporters are coated with polymeric coatings. Optionally, these reporter or label nanoparticles may be Raman-active, such that they provide a distinctive Raman signature upon excitation with electromagnetic radiation.

    摘要翻译： 本发明的实施方案提供了用聚合物涂层和纳米颗粒涂覆聚合物涂层的方法。 聚合物涂层通常包含两层或更多层，其中第一层具有与第二层相反的电荷。 在另外的实施方案中，可以用作标记或报告物的纳米颗粒涂覆有聚合物涂层。 任选地，这些报告物或标记纳米颗粒可以是拉曼活性的，使得它们在用电磁辐射激发时提供独特的拉曼特征。

    Programmable molecular barcodes
    5.
    发明申请
    Programmable molecular barcodes 审中-公开
    可编程分子条形码

    公开(公告)号：US20070054288A1

    公开(公告)日：2007-03-08

    申请号：US11430612

    申请日：2006-05-08

    申请人： Xing Su ,  Tae-Woong Koo ,  Andrew Berlin ,  Lei Sun ,  Narayanan Sundararajan ,  Mineo Yamakawa

    发明人： Xing Su ,  Tae-Woong Koo ,  Andrew Berlin ,  Lei Sun ,  Narayanan Sundararajan ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  G06K7/10

    CPC分类号： B82Y10/00 ,  B82Y5/00 ,  C12Q1/6816 ,  G06K19/06028 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/1025

    摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

    摘要翻译： 本公开涉及用于生产和/或使用分子条形码的方法。 在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。 标签可以附加到骨干和/或分支结构。 条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。 可以通过标签的类型和位置区分不同的条形码。 在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。 标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。 在替代实施例中，条形码可以通过单体单元的聚合来制备。 可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

    Multiplex data collection and analysis in bioanalyte detection
    6.
    发明申请
    Multiplex data collection and analysis in bioanalyte detection 有权
    生物分析仪检测中的多重数据收集和分析

    公开(公告)号：US20070048746A1

    公开(公告)日：2007-03-01

    申请号：US11216112

    申请日：2005-09-01

    申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

    发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

    摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a green spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

    摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（各自由唯一报道分子标记）与样品接触，并采用绿色光谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

    Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning
    9.
    发明申请
    Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning 审中-公开
    使用肽或核酸微图案生产碳纳米管的方法

    公开(公告)号：US20050151126A1

    公开(公告)日：2005-07-14

    申请号：US10750141

    申请日：2003-12-31

    申请人： Mineo Yamakawa ,  Yeugang Zhang ,  Xing Su ,  Lei Sun ,  Andrew Berlin ,  Narayanan Sundararajan

    发明人： Mineo Yamakawa ,  Yeugang Zhang ,  Xing Su ,  Lei Sun ,  Andrew Berlin ,  Narayanan Sundararajan

    IPC分类号： C01B31/02 ,  D01F9/127 ,  H01L51/00 ,  H01L51/30 ,  D01F9/12 ,  H01L29/06

    CPC分类号： B82Y40/00 ,  B82Y10/00 ,  B82Y30/00 ,  C01B32/162 ,  C01B2202/08 ,  C01B2202/36 ,  D01F9/127 ,  H01L51/0048 ,  H01L51/0052

    摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles 140, 230 to polymer 120, 210 molecules, attaching the polymer 120, 210 molecules to a substrate, removing the polymer 120, 210 molecules and producing carbon nanotubes on the catalyst nanoparticles 140, 230. The polymer 120, 210 molecules can be attached to the substrate in ordered patterns, using self-assembly or molecular alignment techniques. The nanotube arrays can be attached to selected areas 110, 310 of the substrate. Within the selected areas 110, 310, the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

    摘要翻译： 本文公开的方法，装置和系统涉及碳纳米管的有序阵列。 在本发明的具体实施方案中，纳米管阵列通过包括将催化剂纳米颗粒140,230连接到聚合物120,210分子，将聚合物120,210分子连接到基底上的方法形成，除去聚合物120,210分子并产生碳 催化剂纳米颗粒140,230上的纳米管。聚合物120,210分子可以使用自组装或分子对准技术以有序图案附着到基底上。 纳米管阵列可以附着到基板的选定区域110,310。 在所选择的区域110,310内，纳米管是非随机分布的。 本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列和包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统的装置。 在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

    External modification of composite organic inorganic nanoclusters
    10.
    发明授权
    External modification of composite organic inorganic nanoclusters 有权
    复合有机无机纳米团簇的外部改性

    公开(公告)号：US07790286B2

    公开(公告)日：2010-09-07

    申请号：US12036869

    申请日：2008-02-25

    申请人： Jingwu Zhang ,  Xing Su ,  Lei Sun

    发明人： Jingwu Zhang ,  Xing Su ,  Lei Sun

    IPC分类号： B32B5/16

    CPC分类号： G01N21/658 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656 ,  Y10S977/773 ,  Y10S977/777 ,  Y10S977/783 ,  Y10T428/12049 ,  Y10T428/2991 ,  Y10T428/2998

    摘要： Modified and functionalized metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. For example, modifications include coatings and layers, such as adsorption layers, metal coatings, silica coatings, and organic layers. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. A metal that enhances the Raman signal from the organic Raman-active compound is inherent in the nanocluster. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

    摘要翻译： 提供了能够提供来自结合在其中的有机拉曼活性分子的增强拉曼信号的改性和官能化的金属纳米团簇。 例如，修饰包括涂层和层，例如吸附层，金属涂层，二氧化硅涂层和有机层。 纳米团簇通常被称为COIN（复合有机无机纳米粒子），并且能够作为敏感的报告物进行分析物检测。 从有机拉曼活性化合物增强拉曼信号的金属在纳米簇中是固有的。 可以将多种有机拉曼活性化合物和化合物的混合物掺入纳米团簇中。