公开(公告)号：US20200071650A1

公开(公告)日：2020-03-05

申请号：US16571552

申请日：2019-09-16

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Heiko Jan van der Linden ,  Adrianus Theodorus Joore ,  Thomas Hankemeier

IPC: C12M1/00 ,  B01L3/00 ,  C12M1/42 ,  C12M3/06 ,  B01F13/00

Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.

公开(公告)号：US20150219594A1

公开(公告)日：2015-08-06

申请号：US14422736

申请日：2013-08-21

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Thomas Hankemeier ,  Jos Quist ,  Kjeld Janssen

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N27/44773

Abstract: The present invention relates to amethod and an apparatus for concentrating, detecting and/or isolating a plurality of charged analytes contained in a sampleby depletion zone isotachophoresis.

Abstract translation: 本发明涉及用于通过耗尽区等速电泳浓缩，检测和/或分离样品中包含的多个带电分析物的方法和装置。

公开(公告)号：US20140274956A1

公开(公告)日：2014-09-18

申请号：US14210147

申请日：2014-03-13

Applicant: Universiteit Leiden ,  Academisch Ziekenhuis Leiden h.o.d.n. LUMC

Inventor： Pieternella Slagboom ,  Thomas Hankemeier

IPC: G01N33/92 ,  A61K31/685 ,  H01J49/00 ,  A61K31/688

CPC classification number: G01N33/92 ,  A61K31/685 ,  A61K31/688 ,  G01N2405/00 ,  G01N2570/00 ,  G01N2800/7042 ,  H01J49/0027

Abstract: The disclosure relates to method of typing a sample from an individual as a sample from an individual with an increased life expectancy, an average life expectancy or a reduced life expectancy. The typing is based on a comparison of a level of lipid species in a sample of the individual to a level of lipid species in a reference. The disclosure further relates to a composition comprising lipid species for use in increasing life expectancy of an individual.

Abstract translation: 本公开涉及从具有预期寿命增加，平均预期寿命或降低的预期寿命的个体的样本中将来自个体的样品分型的方法。 分型是基于个体样品中脂质物质与参考文献中脂质物质水平的比较。 本公开还涉及包含用于增加个体的预期寿命的脂质的组合物。

公开(公告)号：US10900008B2

公开(公告)日：2021-01-26

申请号：US16571552

申请日：2019-09-16

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Heiko Jan van der Linden ,  Adrianus Theodoras Joore ,  Thomas Hankemeier

IPC: C12M1/00 ,  B01F13/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/42

Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.

公开(公告)号：US09575031B2

公开(公告)日：2017-02-21

申请号：US14422736

申请日：2013-08-21

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Thomas Hankemeier ,  Jos Quist ,  Kjeld Janssen

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N27/44773

Abstract: The present invention relates to a method and an apparatus for concentrating, detecting and/or isolating a plurality of charged analytes contained in a sample by depletion zone isotachophoresis.

Abstract translation: 本发明涉及用于通过耗尽区等速电泳浓缩，检测和/或分离样品中包含的多个带电分析物的方法和装置。

公开(公告)号：US20150075300A1

公开(公告)日：2015-03-19

申请号：US14384943

申请日：2013-03-12

Applicant: UNIVERSITEIT LEIDEN

Inventor： Thomas Hankemeier ,  Jan-Willem Schoonen ,  Heiko Jan Van Der Linden ,  Paul Vulto

IPC: G01N30/12 ,  G01N30/62 ,  G01N30/46

CPC classification number: G01N30/12 ,  B01D1/00 ,  G01N1/2214 ,  G01N1/2273 ,  G01N1/24 ,  G01N1/4022 ,  G01N1/405 ,  G01N30/463 ,  G01N30/62 ,  G01N2001/2223 ,  G01N2001/4027 ,  G01N2030/027 ,  G01N2030/126

Abstract: The present invention relates to a device and an arrangement for selective solvent evaporation from a liquid feed, the feed comprising one or more components diluted in at least a first solvent or a solvent blend, comprising: a) a first tubular vessel having a distal end or a channel suitable for the formation of a droplet of a first volume, at an inflow rate n, at the tip or in the lumen of the tubular vessel, and b) means for subjecting the droplet to a solvent removal step at an evaporation rate r2 to evaporate at least part of the first solvent or solvent blend, to accumulate the components in the feed in the droplet during the evaporation process at an accumulation rate r3, to obtain a concentrated feed volume in a concentrated droplet, wherein the evaporation and/or inflow rates are continuously adjusted to achieve a desired accumulation rate. The invention further relates to a method for selective solvent removal from an analyte mixture obtained in a chromatographic separation process.

Abstract translation: 本发明涉及一种用于从液体进料选择性溶剂蒸发的装置和装置，所述进料包含在至少第一溶剂或溶剂共混物中稀释的一种或多种组分，所述组分包括：a）第一管状容器，其具有远端 或适于在管状容器的尖端或内腔中以流入速率n形成第一体积的液滴的通道，以及b）用于使液滴以蒸发速率进行溶剂去除步骤的装置 r2蒸发至少部分第一溶剂或溶剂共混物，以在堆积速率r3的蒸发过程中将液体中的组分积聚在液滴中，以获得浓缩液滴中的浓缩进料体积，其中蒸发和/ 或流入速率连续调整以达到所需的累积率。 本发明还涉及从色谱分离过程中获得的分析物混合物中选择性溶剂去除的方法。

公开(公告)号：US12072322B2

公开(公告)日：2024-08-27

申请号：US17293978

申请日：2019-11-18

Applicant: UNIVERSITEIT LEIDEN

Inventor： Thomas Hankemeier ,  Anne Charlotte Dubbelman ,  Amy C. Harms ,  Oskar González

IPC: G01N30/72 ,  G01N30/04 ,  G01N30/86 ,  G01N30/02

CPC classification number: G01N30/7266 ,  G01N30/04 ,  G01N30/8631 ,  G01N30/8658 ,  G01N2030/025 ,  G01N2030/045

Abstract: The present invention relates to a method for quantifying one or more analytes in a sample by an analysis system comprising a separation unit (LC column), a means of adding a solution post-column (Connector), and a detection unit comprising a mass spectrometer coupled through an ionization source, the method comprising: ⋅ (i) inducing matrix effect on the analytes in the sample and on the post-column infused internal standards (PCI-ISs); ⋅ (ii) matching one or more post-column infused internal standard (PCI-IS) to each analyte that best matches the analyte's response to the matrix effect, and ⋅ (iii) storing the analyte-matched PCI-IS identification and, optionally, associated response data in a library; and ⋅ (iv) applying the analyte-matched PCI-IS to the analyte in other samples to correct the analyte peak responses for the matrix effect during ionization and to obtain (absolute) quantitation of the analyte using the response data.

公开(公告)号：US11504645B2

公开(公告)日：2022-11-22

申请号：US14898813

申请日：2014-06-19

Applicant: UNIVERSITEIT LEIDEN

Inventor： Jan-Willem Schoonen ,  Peter Lindenburg ,  Vincent Van Duinen ,  Amar Oedit ,  Paul Vulto ,  Thomas Hankemeier

IPC: B01D11/04 ,  G01N1/40 ,  B01L3/00

Abstract: The present invention relates to a process for the electro extraction of molecules from a moving fluid donor phase into an acceptor phase, comprising the steps of: providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode, providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode; and providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and (d) applying an electrical field between the first and the second electrode.

公开(公告)号：US10006889B2

公开(公告)日：2018-06-26

申请号：US14898833

申请日：2014-06-19

Applicant: UNIVERSITEIT LEIDEN

Inventor： Jan-Willem Schoonen ,  Peter Lindenburg ,  Paul Vulto ,  Thomas Hankemeier

IPC: G01N30/12 ,  G01N1/28 ,  G01N1/34 ,  G01N1/40 ,  G01N30/06 ,  G01N30/64 ,  G01N30/72 ,  G01N30/46

CPC classification number: G01N30/12 ,  G01N1/28 ,  G01N1/34 ,  G01N1/4022 ,  G01N30/06 ,  G01N30/463 ,  G01N30/64 ,  G01N30/7233

Abstract: The present invention relates to a droplet receiver, comprising a receptacle for receiving a droplet, a fluid conduit connected at a first end thereof to the droplet receptacle thereby providing a course within which the droplet received in the droplet receptacle moves, the conduit at its distal end in communication with a reservoir, and a fluid layer wetting the internal walls of the receptacle and conduit in such a way as permit reception of the droplet without loss of the received droplet due to wetting of the internal receptacle and conduit walls.

公开(公告)号：US09919317B2

公开(公告)日：2018-03-20

申请号：US14394895

申请日：2013-04-19

Applicant: Universiteit Leiden

Inventor： Robert-Jan Raterink ,  Peter Lindenburg ,  Thomas Hankemeier

IPC: B03C5/02 ,  B01D11/04 ,  B01D61/38 ,  G01N1/40 ,  B01D57/02 ,  B01D61/24 ,  B01D61/42 ,  G01N27/447 ,  G01N35/10 ,  G01N30/00 ,  G01N30/06

CPC classification number: B03C5/02 ,  B01D11/0419 ,  B01D57/02 ,  B01D61/246 ,  B01D61/38 ,  B01D61/42 ,  G01N1/4005 ,  G01N27/44756 ,  G01N2001/4011 ,  G01N2001/4038 ,  G01N2030/009 ,  G01N2030/062 ,  G01N2035/1053

Abstract: The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode.