公开(公告)号：US12072322B2

公开(公告)日：2024-08-27

申请号：US17293978

申请日：2019-11-18

Applicant: UNIVERSITEIT LEIDEN

Inventor： Thomas Hankemeier ,  Anne Charlotte Dubbelman ,  Amy C. Harms ,  Oskar González

IPC: G01N30/72 ,  G01N30/04 ,  G01N30/86 ,  G01N30/02

CPC classification number: G01N30/7266 ,  G01N30/04 ,  G01N30/8631 ,  G01N30/8658 ,  G01N2030/025 ,  G01N2030/045

Abstract: The present invention relates to a method for quantifying one or more analytes in a sample by an analysis system comprising a separation unit (LC column), a means of adding a solution post-column (Connector), and a detection unit comprising a mass spectrometer coupled through an ionization source, the method comprising: ⋅ (i) inducing matrix effect on the analytes in the sample and on the post-column infused internal standards (PCI-ISs); ⋅ (ii) matching one or more post-column infused internal standard (PCI-IS) to each analyte that best matches the analyte's response to the matrix effect, and ⋅ (iii) storing the analyte-matched PCI-IS identification and, optionally, associated response data in a library; and ⋅ (iv) applying the analyte-matched PCI-IS to the analyte in other samples to correct the analyte peak responses for the matrix effect during ionization and to obtain (absolute) quantitation of the analyte using the response data.