公开(公告)号：US10900008B2

公开(公告)日：2021-01-26

申请号：US16571552

申请日：2019-09-16

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Heiko Jan van der Linden ,  Adrianus Theodoras Joore ,  Thomas Hankemeier

IPC: C12M1/00 ,  B01F13/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/42

Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.

公开(公告)号：US09429249B2

公开(公告)日：2016-08-30

申请号：US14465345

申请日：2014-08-21

Applicant: Universiteit Leiden

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Ender Yildirim

IPC: B01L99/00 ,  F16K99/00 ,  B01L3/00

CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2400/0406 ,  B01L2400/0688 ,  F16K2099/0084 ,  Y10T137/7859

Abstract: The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus.

Abstract translation: 本发明涉及一种用于控制一种或多种流体流动的流体可触发的被动阀，包括：一个体积，包括至少两个用于分别固定第一和第二流体 - 液体弯液面的毛细管压力屏障，两个毛细管压力屏障将体积分成 至少三个子体积，其中两个子体积位于上游，一个子体积相对于每个相应的流体 - 液体弯液面的前进方向位于下游; 其中所述毛细管压力屏障间隔开，使得当在第一毛细管压力屏障上锁定第一流体 - 液体弯液面并且在第二毛细管压力屏障上或在第二毛细管压力屏障上到达或钉住第二流体 - 液体弯月面时，两个半月板触摸和 从而聚结成一个流体 - 液体弯液面。

公开(公告)号：US11344877B2

公开(公告)日：2022-05-31

申请号：US14426920

申请日：2013-09-10

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Ender Yildirim

IPC: B01L3/00

Abstract: The present invention relates to an apparatus for controlling the shape and/or position of a moveable fluid-fluid meniscus, and methods of use, in particular a method to control the shape of a moveable fluid-fluid meniscus in an apparatus in which the meniscus is caused to align along a stable capillary barrier or phaseguide.

公开(公告)号：US20200071650A1

公开(公告)日：2020-03-05

申请号：US16571552

申请日：2019-09-16

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Heiko Jan van der Linden ,  Adrianus Theodorus Joore ,  Thomas Hankemeier

IPC: C12M1/00 ,  B01L3/00 ,  C12M1/42 ,  C12M3/06 ,  B01F13/00

Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.

公开(公告)号：US20150238952A1

公开(公告)日：2015-08-27

申请号：US14426920

申请日：2013-09-10

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Ender Yildirim

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L2400/088

Abstract: The present invention relates to an apparatus for controlling the shape and/or position of a moveable fluid-fluid meniscus, the apparatus comprising a volume for containing and directing fluid, the filling direction being a downstream direction, including the meniscus and the volume having at least a first structure defining a capillary pressure barrier along which the meniscus tends to align, the capillary pressure barrier and the meniscus defining a boundary in the volume between at least two sub-volumes,wherein (a) the capillary pressure barrier is stabilized by subtending at both ends an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90°, while not providing a deliberate fluid alignment weakness along the capillary pressure barrier that reduces the stability of the capillary pressure barrier and/or (b) wherein the capillary pressure is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fluid-fluid meniscus upon alignment along the capillary pressure barrier in the absence of the stretching barrier, (c) the capillary pressure barrier is stabilized by subtending at one end an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90°, and at the other end is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fluid-fluid meniscus upon alignment along the capillary pressure barrier in the absence of the stretching barrier; wherein the stretching barrier is shaped such that at least one directional component is orthogonal to the capillary pressure barrier.

Abstract translation: 本发明涉及一种用于控制可移动流体 - 液体弯液面的形状和/或位置的装置，该装置包括用于容纳和引导流体的容积，填充方向是下游方向，包括弯月面和体积具有 至少一个限定毛细管压力屏障的第一结构，所述弯月面倾向于沿其对准，所述毛细管压力屏障和所述弯液面在至少两个子体积之间限定所述体积中的边界，其中（a）毛细管压力屏障通过对 在两端处，与毛细管压力屏障的下游侧的体积壁成一角度大于90°，​​而不会沿着毛细管压力屏障提供有意的流体排列弱点，这降低了毛细管压力屏障的稳定性， /或（b）其中通过在小于最大str的距离处提供拉伸屏障来稳定毛细管压力 在不存在拉伸屏障的情况下沿着毛细管压力屏障对准流体 - 液体弯液面的蚀刻距离，（c）通过在一端与一个下游侧的体积的壁成一角度来稳定毛细管压力屏障 的毛细管压力屏障大于90°，​​并且在另一端通过在不存在毛细管压力屏障的情况下沿着毛细管压力屏障对准时在比液体液体弯液面的最大拉伸距离小的距离处提供拉伸屏障来稳定 拉伸屏障; 其中所述拉伸屏障被成形为使得至少一个方向分量正交于所述毛细管压力屏障。

公开(公告)号：US20150040999A1

公开(公告)日：2015-02-12

申请号：US14465345

申请日：2014-08-21

Applicant: Universiteit Leiden

Inventor： Paul VULTO ,  Sebastiaan Johannes Trietsch ,  Ender Yildirim

IPC: F16K99/00

CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2400/0406 ,  B01L2400/0688 ,  F16K2099/0084 ,  Y10T137/7859

Abstract: The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus

Abstract translation: 本发明涉及一种用于控制一种或多种流体流动的流体可触发的被动阀，包括：一个体积，包括至少两个用于分别固定第一和第二流体 - 液体弯液面的毛细管压力屏障，两个毛细管压力屏障将体积分成 至少三个子体积，其中两个子体积位于上游，一个子体积相对于每个相应的流体 - 液体弯液面的前进方向位于下游; 其中所述毛细管压力屏障间隔开，使得当在第一毛细管压力屏障上锁定第一流体 - 液体弯液面并且在第二毛细管压力屏障上或在第二毛细管压力屏障上到达或钉住第二流体 - 液体弯月面时，两个半月板触摸和 从而聚结成一个流体 - 液体弯液面