公开(公告)号：US4282001A

公开(公告)日：1981-08-04

申请号：US26851

申请日：1979-04-04

申请人： Sigmar Klose ,  Herbert Buschek ,  Helmut Schlumberger

发明人： Sigmar Klose ,  Herbert Buschek ,  Helmut Schlumberger

IPC分类号： B01J20/22 ,  C12Q1/54 ,  C12Q1/60 ,  C12Q1/62 ,  G01N1/34 ,  G01N31/00 ,  G01N33/04 ,  G01N33/48 ,  G01N33/96 ,  G01N33/52 ,  G01N33/66 ,  G01N33/92

CPC分类号： G01N33/96 ,  C12Q1/54 ,  C12Q1/60 ,  C12Q1/62 ,  G01N1/34 ,  G01N33/04 ,  Y10T436/25

摘要： For the removal of turbidities in reaction mixtures clouded by sample material, especially for photometric measurement, there are added to the reaction mixture:(a) at least one low molecular weight organic compound, such as an aromatic alcohol or amine, which contains an electronegative substituent, for example a phenol or naphthol, which is substituted with one or more halogen atoms and/or hydroxyl groups and(b) at least one detergent which dissolves to give without compound (a), a clear solution and which forms a sparingly soluble complex with component (a), such as a non-ionic polyethylene oxide adduct or a cationic detergent, especially an alkyl, aralkyl or alkylthio ether or an alkyl or aralkyl ester of a polyethylene oxide glycol containing 8 to 22 carbon atoms in the alkyl radical and 5 to 25 ethylene oxide units or an alkyl dimethyl benzyl ammonium chloride compound containing 8 to 22 carbon atoms in the alkyl radical,in an amount sufficient for the appearance of a precipitate of the complex, whereafter further detergent is added thereto until the precipitated complex dissolves again.

摘要翻译： 对于通过样品材料（特别是用于光度测量）混浊的反应混合物中的浊度的去除，加入到反应混合物中：（a）至少一种低分子量有机化合物，例如芳族醇或胺，其含有电负性 取代基，例如苯酚或萘酚，其被一个或多个卤素原子和/或羟基取代，和（b）至少一种在没有化合物（a）的情况下溶解而得到的洗涤剂，澄清溶液并形成微溶于 与组分（a）的复合物，例如非离子聚环氧乙烷加合物或阳离子洗涤剂，特别是在烷基中含有8-22个碳原子的聚环氧乙烷的烷基，芳烷基或烷硫基醚或烷基或芳烷基酯 和5至25个环氧乙烷单元或烷基中含有8至22个碳原子的烷基二甲基苄基氯化铵化合物，其量足以出现沉淀物 然后向其中加入另外的洗涤剂，直到沉淀的复合物再次溶解。

公开(公告)号：US4915911A

公开(公告)日：1990-04-10

申请号：US98780

申请日：1987-09-21

申请人： Sigmar Klose ,  Manfred Pasch ,  Helmut Schlumberger ,  Wolfgang Kleeman ,  Friedhelm Vieth

发明人： Sigmar Klose ,  Manfred Pasch ,  Helmut Schlumberger ,  Wolfgang Kleeman ,  Friedhelm Vieth

IPC分类号： G01N33/48 ,  B01J4/00 ,  B01L3/00 ,  G01N21/07 ,  G01N33/543 ,  G01N35/00

CPC分类号： G01N21/07 ,  B01L3/5027

摘要： The present invention provides a device for rinsing out a substance present in a zone (d) with a liquid rinsing agent in several rinsing steps and for transferring the rinsing agent emerging from the zone (d) in the last rinsing step to a cuvette (K), wherein the zone (d) is present on a rotor (R) of variable speed of rotation, radially inwardly of the zone (d) on the rotor (R) there is provided a pump chamber (PK) for the rinsing agent which is connected with the zone (d) via a valve chamber (VK2) which fills, under the action of centrifugal force, above a predetermined speed of rotation, from the pump chamber (PK) and empties below the predetermined speed of rotation into the zone (d) and the zone (d) is connected with the cuvette (K) via a pipe (4) which, in one section, the axis of which has a component directed radially outwardly, is directed into a waste chamber (AK).

摘要翻译： 本发明提供了一种用于在几个漂洗步骤中用液体漂洗剂冲洗出区域（d）中存在的物质的装置，并用于将在最后一个漂洗步骤中从（d）区域出来的漂洗剂转移到比色皿 ），其中所述区域（d）存在于所述转子（R）上的所述区域（d）的径向向内的可变速度转子（R）上，其中设置有用于所述漂洗剂的泵室（PK），所述泵室 通过阀室（VK2）与区域（d）连接，阀室（VK2）在离心力的作用下从泵室（PK）溢出超过预定转速，并且在预定转速下排空到区域 （d）和区域（d）经由管道（4）与试管（K）连接，管道（4）在其一个部分中具有径向向外指向的部件被引导到废物室（AK）中。

公开(公告)号：US4690899A

公开(公告)日：1987-09-01

申请号：US751368

申请日：1985-07-02

申请人： Sigmar Klose ,  Manfred Pasch ,  Helmut Schlumberger ,  Wolfgang Kleemann ,  Friedhelm Vieth

发明人： Sigmar Klose ,  Manfred Pasch ,  Helmut Schlumberger ,  Wolfgang Kleemann ,  Friedhelm Vieth

IPC分类号： G01N33/48 ,  G01N21/07 ,  G01N33/543 ,  G01N33/563 ,  G01N33/68 ,  G01N21/11

CPC分类号： G01N21/07 ,  G01N33/54366 ,  G01N33/563 ,  G01N33/6857 ,  Y10T436/111666

摘要： In a process for carrying out an analytical determination by mixing and incubating a sample liquid with at least one dry reagent which is soluble therein to form a reaction mixture liquid and measuring a parameter in the reaction mixture liquid, the sample liquid is first transported along a transport path to the soluble dry reagent and the resulting reaction mixture liquid then further transported along the transport path. The further transporting of the reaction mixture liquid along the transport path is under the influence of a force-change cycle in which centrifugal force and a further force alternately predominate to determine the direction of the further transporting along the transport path. At least one further liquid is transported on another path, part of which is separate from and part of which is common with the transport path, by the force-change cycle in such a manner that at least two of the liquids pass through the common part of the transport path chronologically separated.

摘要翻译： 在通过将样品液体与可溶于其中的至少一种干试剂混合并孵育以形成反应混合物液体并测量反应混合物液体中的参数的方法进行分析测定的过程中，首先将样品液体沿着 向可溶性干试剂的输送路径和所得到的反应混合物液体进一步沿输送路径输送。 反应混合物液体沿着输送路径的进一步输送受力变化循环的影响，其中离心力和进一步的力交替占主导地位，以确定沿输送路径进一步输送的方向。 至少一个另外的液体在另一个路径上被传送，其中一部分液体通过力 - 变化循环分开并且其一部分与输送路径共同，使得至少两个液体通过公共部分 的运输路径按时间顺序分隔。

公开(公告)号：US4476223A

公开(公告)日：1984-10-09

申请号：US452690

申请日：1982-12-23

申请人： Herbert Buschek ,  Helmut Schlumberger ,  Joachim Siedel

发明人： Herbert Buschek ,  Helmut Schlumberger ,  Joachim Siedel

IPC分类号： C12N20060101 ,  C12N9/16 ,  C12N9/18 ,  C12N9/96 ,  C12Q20060101 ,  C12Q1/44 ,  C12Q1/46 ,  C12Q1/60 ,  C12Q1/61 ,  C12R1/38 ,  C12R1/39

CPC分类号： C12N9/96 ,  C12Q1/60

摘要： The present invention provides a process for the stabilization of an aqueous solution of cholesterol esterase from Pseudomonas, especially in the presence of a surface-active agent, wherein the enzyme is dissolved in a phosphate-free buffer which contains 10 to 200 mMol/liter of magnesium ions.

摘要翻译： 本发明提供了一种稳定来自假单胞菌属的胆固醇酯酶水溶液的方法，特别是在表面活性剂存在下，其中酶溶解在无磷酸盐缓冲液中，该缓冲液含有10〜200mMol / 镁离子。

公开(公告)号：US5356034A

公开(公告)日：1994-10-18

申请号：US8552

申请日：1993-01-22

申请人： Helmut Schlumberger

发明人： Helmut Schlumberger

IPC分类号： B05C5/00 ,  B01J4/02 ,  B01L3/02 ,  B01L99/00 ,  G01F11/02 ,  G01N1/00 ,  G01N1/14 ,  G01N1/16 ,  G01N1/18 ,  G01N1/20 ,  G01N35/10 ,  B67D5/08

CPC分类号： G01F11/021 ,  B01J4/02 ,  B01L3/0293 ,  G01F11/02 ,  G01N2035/1041

摘要： Apparatus for proportioned feeding of an analysis fluid (7) onto a target (5), ejects fluid onto the target in small quantities pulse-wise out of a nozzle (2) through a nozzle outlet opening (3). The apparatus allows the precise proportioning of analysis fluid quanta which are substantially larger than in the case of the "drop on demand" methods commonly used to date for analysis fluids, but are smaller than the minimum doses achievable to date with diluters and dispensers. The apparatus includes a pressure chamber (1) in which the analysis fluid is held under pressure, and a valve unit (11) with a valve opening. A closing element is moved by a positioning element. The valve unit (11) is constructed so that the ejection of the fluid is supported by the movement of the closing element (13) during the closure of the valve opening (23).

摘要翻译： 用于将分析流体（7）成比例地供给到目标（5）上的装置，通过喷嘴出口（3）从喷嘴（2）中以脉冲方式将流体喷射到目标上。 该装置允许分析流体量子的精确配比，其大大比在分析流体通常使用的“按需滴下”方法的情况下更大，但是小于稀释剂和分配器迄今可达到的最小剂量。 该装置包括压力室（1），其中分析流体保持在压力下，以及具有阀开口的阀单元（11）。 闭合元件由定位元件移动。 阀单元（11）被构造成使得在阀开口（23）的关闭期间，通过关闭元件（13）的运动来支撑流体的排出。

公开(公告)号：US06431745B1

公开(公告)日：2002-08-13

申请号：US09674257

申请日：2001-03-12

申请人： Helmut Schlumberger

发明人： Helmut Schlumberger

IPC分类号： B01F1100

CPC分类号： B01F11/0002 ,  B01F9/10 ,  G01N35/0098 ,  G01N2035/00524

摘要： A holder containing a mixing vessel is rotatable about its longitudinal axis by a drive configured to rotate the holder alternately in a clockwise and anti-clockwise direction. A programmable control unit is configured to regulate an angular excursion (&agr;) of the holder in the clockwise and anti-clockwise direction. The control unit includes instructions for a mixing sequence to be carried out in which the holder carries out first angular excursions of a first angle in a first cycle and second angular excursions of a second angle in a second cycle. The process includes placing liquids and/or solids into the mixing vessel, and then inserting the mixing vessel into the holder. The holder is subsequently alternately rotated by the drive through the first angular excursions and then the second angular excursions.

摘要翻译： 包含混合容器的保持器可通过驱动器围绕其纵向轴线旋转，所述驱动器被配置成沿着顺时针和逆时针方向交替地旋转保持器。 可编程控制单元被配置成调节保持器在顺时针和逆时针方向上的角度偏移（α）。 控制单元包括要执行的混合顺序的指令，其中保持器在第一周期中执行第一角度的第一角度偏移和在第二周期中执行第二角度的第二角度偏移。 该方法包括将液体和/或固体放入混合容器中，然后将混合容器插入保持器。 随后，保持器通过第一角度偏移和第二角度偏移由驱动器交替地旋转。

公开(公告)号：US4503144A

公开(公告)日：1985-03-05

申请号：US328350

申请日：1981-12-07

申请人： Rolf Deeg ,  Helmut Schlumberger ,  Joachim Ziegenhorn

发明人： Rolf Deeg ,  Helmut Schlumberger ,  Joachim Ziegenhorn

IPC分类号： G01N33/50 ,  C12Q1/60 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12R1/465

CPC分类号： C12Q1/60 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/886

摘要： The present invention provides a process for the determination of cholesterol in free or bound form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol.The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro-organism of the genus Streptomyces.

摘要翻译： 本发明提供了一种通过胆固醇氧化酶和任选的胆固醇酯酶通过测量所形成的过氧化氢或形成的胆碱酯的氧消耗来测定游离或结合形式的胆固醇的方法，其中进行测定 动态地使用已经从链霉菌属的微生物获得的胆固醇氧化酶，并加入3,4-二氯苯酚。 本发明还提供用于胆固醇的动力学测定的试剂，其包含胆固醇氧化酶和用于测定过氧化氢或胆石素和任选的胆固醇酯酶的系统，其中还含有3,4-二氯苯酚，所用的胆固醇氧化酶源自 链霉菌属的微生物。

公开(公告)号：US4845030A

公开(公告)日：1989-07-04

申请号：US122600

申请日：1987-11-20

申请人： Hans-Georg Batz ,  Rupert Herrmann ,  Fritz Topfmeier ,  Helmut Schlumberger

发明人： Hans-Georg Batz ,  Rupert Herrmann ,  Fritz Topfmeier ,  Helmut Schlumberger

IPC分类号： G01N33/52 ,  C09B57/00 ,  C12Q1/28 ,  G01N21/77 ,  G01N21/78 ,  G01N33/50

CPC分类号： C12Q1/28 ,  C12Q2326/00 ,  Y10T436/144444 ,  Y10T436/206664

摘要： The present invention is concerned with the use of aniline derivatives of the general formula: ##STR1## wherein R.sup.1 is a hydrogen atom or a --(CH.sub.2).sub.n --X radical, in which n is a whole number of from 1 to 3, X is H, OH, NH.sub.2, CH.sub.3 CONH, CH.sub.3 SO.sub.2 NH, COOH, SO.sub.3 H or --ArSO.sub.3 H, Ar being an optionally substituted arylene radical, m is a whole number of from 2 to 4, R.sup.3 is a hydrogen atom, an alkyl radical containing up to 3 carbon atoms, OCH.sub.3, CH.sub.3 CONH, COOH or SO.sub.3 H and R.sup.4 is a hydrogen, chlorine or bromine atom or a carboxylic acid or sulphonic acid group, as coupling components in oxidative color formation reactions.The present invention also provides an agent for the analytical determination of oxidizing substances by oxidative color coupling which, as coupling component, contains at least one compound of general formula (I).

摘要翻译： 本发明涉及以下通式的苯胺衍生物的用途：其中R 1为氢原子或 - （CH 2）n X基团，其中n为1至3的整数， X是H，OH，NH 2，CH 3 CONH，CH 3 SO 2 NH，COOH，SO 3 H或-ArSO 3 H，Ar是任选取代的亚芳基，m是整数2至4，R 3是氢原子， 3个碳原子，OCH 3，CH 3 CONH，COOH或SO 3 H，R 4是氢，氯或溴原子或羧酸或磺酸基团，作为氧化颜色形成反应中的偶联组分。 本发明还提供了一种通过氧化颜色偶联分析测定氧化物质的试剂，其作为偶联组分含有至少一种通式（I）的化合物。

公开(公告)号：US4460683A

公开(公告)日：1984-07-17

申请号：US394562

申请日：1982-07-02

申请人： Manfred Gloger ,  Josef Heinle ,  Helmut Schlumberger

发明人： Manfred Gloger ,  Josef Heinle ,  Helmut Schlumberger

IPC分类号： C12N9/00 ,  C12N9/06 ,  C12N11/08 ,  C12N11/10 ,  C12Q1/26 ,  C12Q1/62 ,  C12N11/02 ,  C12N11/06

CPC分类号： C12N11/10 ,  C12N11/08 ,  C12Q1/62

摘要： The present invention provides liver uricase, wherein it is covalently bond to a water-soluble polysaccharide, polyacid anhydride, polyvinylpyrrolidone or acid anhydride as carrier substance and is still soluble at pH values below 6.The present invention also provides a process for the preparation of this liver uricase.

摘要翻译： 本发明提供肝尿酸酶，其中其与水溶性多糖，多酸酐，聚乙烯吡咯烷酮或酸酐共价键合作为载体物质，并且在pH值低于6时仍然是可溶的。本发明还提供了制备 这种肝尿酸酶。