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    Mesylate based master mix
    1.
    发明授权
    Mesylate based master mix 有权

    公开(公告)号：US11293059B2

    公开(公告)日：2022-04-05

    申请号：US16623975

    申请日：2018-06-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chang Zhong ,  Julio Mulero ,  Steven Menchen

    IPC: C12Q1/686 ,  C12Q1/6848

    Abstract: Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.

    Thiolated nucleotide analogues for nucleic acid synthesis
    3.
    发明授权
    Thiolated nucleotide analogues for nucleic acid synthesis 有权

    公开(公告)号：US10344325B2

    公开(公告)日：2019-07-09

    申请号：US14937211

    申请日：2015-11-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Steven Menchen ,  Theo Nikiforov ,  Barnett Rosenblum

    IPC: C12Q1/68 ,  C12Q1/6869 ,  G01N27/414

    Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

    Thiolated nucleotide analogues for nucleic acid synthesis
    7.
    发明授权
    Thiolated nucleotide analogues for nucleic acid synthesis 有权

    公开(公告)号：US11047004B2

    公开(公告)日：2021-06-29

    申请号：US16421284

    申请日：2019-05-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Steven Menchen ,  Theo Nikiforov ,  Barnett Rosenblum

    IPC: C12Q1/6869 ,  G01N27/414

    Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

    Solid Phases Optimized for Chemiluminescent Detection
    8.
    发明申请
    Solid Phases Optimized for Chemiluminescent Detection 审中-公开
    固化相用于化学发光检测

    公开(公告)号：US20140186831A1

    公开(公告)日：2014-07-03

    申请号：US14070138

    申请日：2013-11-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brooks Edwards ,  Timothy Geiser ,  Steven Menchen ,  Alison Sparks ,  John Voyta

    IPC: C12Q1/68

    CPC classification number: C12Q1/6823 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  G01N33/54393 ,  Y10S436/805 ,  Y10S436/823

    Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.

    Abstract translation: 提供了化学发光测定的固体支持物。 固体支持物包括共价或物理连接到支持物表面上的多个探针和结合到表面或载体的大部分中的化学发光增强部分。 固体支持物可以是包含邻近阳离子微凝胶层的上探针结合层（例如，吖内酯聚合物层或多孔功能性聚酰胺层）的多层载体。 吖内酯官能聚合物可以是与乙二胺交联的二甲基丙烯酰胺和乙烯基内酯的共聚物。 阳离子微凝胶层可以是交联的含季铵盐的聚合物。 还提供了使用固体支持物进行化学发光测定的方法和试剂盒。 试剂盒包括二氧杂环丁烷底物，生物聚合物探针 - 酶复合物和固体支持物。 固体支持物可以是与阳离子微凝胶层相邻的吖内酯官能聚合物层; 与阳离子微凝胶层相邻的多孔聚酰胺官能层; 或季铵化的吖内酯官能聚合物层。

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